1. INTRODUCTION
Triticale (X Triticosecale Wittmack) is the first artificial species developed by crossing wheat and rye. This crop was to combine the high yield and nutritional value of wheat with the hardiness and resistance of rye. However, triticale is characterized by some unfavorable traits. One of them is preharvest sprouting (PHS) which is typical for mild and humid climate. PHS results in considerable yield and grade losses [1]. To develop resistant cultivars is one of the most appropriate solutions of this serious problem. However, the selection for PHS resistance is complicated, because this trait is influenced by the genotype, environmental factors and their interaction [2]. Hence, the development of the reliable methods to select for PHS resistance is of great relevance for triticale breeders.
PHS is caused by the termination of dormancy in immature seeds in the ear which leads to the initiation of embryo growth. Dormancy is supposed to have evolved as a mechanism to escape adverse environmental conditions and intravarietal competition. In contrast, dormancy period was reduced by the domestication of species which was accompanied by the selection for uniform and rapid germination to achieve good stands [3]. Seed dormancy in wheat was shown to be associated with the level of abscisic and gibberellic acids, α-amylase activity, structure of spike and awns and presence of water-soluble inhibitors of germination in the bracts of spikes and seed [1,4-6]. Since red seed color in wheat is associated with the higher level of dormancy [7], it has been used as a marker for PHS resistance. It might be due either to the tight genetic linkage or the pleiotropic effect of the genes responsible for grain color to PHS [8]. Another genetic factor with a major impact on seed dormancy is the gene VIVIPAROUS (Vp) which homologs Vp-1A, Vp-1B and Vp-1D were isolated and cloned in wheat [9-11]. The product of the gene was shown to be an incorrectly spliced transcriptional factor determining seed desiccation, dormancy and embryo sensitivity to abscisic acid. According to the recently obtained results, the level of the Vp-1B transcription in the genotypes with 193 bp insertion (the Vp-1Bb allele) and 83 bp deletion (the Vp-1Bb allele) is higher then in the wheat genotype without them (the Vp-1Bb allele) [11]. The analysis of the European wheat cultivars revealed that besides the mentioned alleles a new allele Vp-1Bd with 25 bp deletion also occurs [12]. The recent analyses of Chinese wheats revealed the new alleles, Vp-1Bf and Vp-1Be, which are associated with high seed dormancy [13,14]. The correlation found between the allelic state of Vp-1B and the PHS in white kernel wheat can be applied in the marker-assisted selection for PHS resistance [11].
For efficient selection of triticale for PHS resistance the robust molecular markers are required. Since the Vp1 gene is present and expressed in triticale [15], the wheat DNA markers of this gene can be transferred to triticale. Recently, the influence of the allelic state of Vp-1B to PHS has been shown in triticale [16].
The aim of this study is to analyze the allelic structure of the core collection of winter hexaploid triticale using STS molecular marker for gene Vp-1B and the potential of its application in MAS-breeding of triticale to PHS resistance.
2. MATERIALS AND METHODS
2.1. Plant Material
The core collection of forty-eight winter triticale lines and cultivars susceptible and resistant to PHS were analyzed (Table 1, resistant accessions are highlighted with grey).
2.2. Seed Color
The intensity of red seed color was estimated according to the standard procedure with modifications by steeping seeds in 5% solution of sodium hydroxide [17].
2.3. DNA Extraction and PCR
DNA was extracted from young leaves according to Bernatzky and Tanksley (1986) [18]. The site tagged sequence (STS) marker for the Viviparous-1B localized to chromosome 3B was used [11]. The PCR was carried out as described in Yang et al. (2007). Amplified PCR fragments were separated on a 1.5% agarose gel at 6 V/cm in 0.5 × TBE buffer, stained with ethidium bromide, and visualized using UV light. “100 bp ladder” (Fermentas) was applied as a size marker.
2.4. Statistical Analysis
To determine the strength of association between two qualitative traits the association coefficient of Pearson and contingency coefficient of Yule were calculated. The association is considered to be significant provided φ ≥ 0.5 or Q ≥ 0.3 [19].
3. RESULTS
3.1. Seed Color and PHS
The color of seed is known to be a polymeric trait because its intensity depends on the number of the dominant
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Table 1. The allelic state of Vp-1B gene of the winter triticale collection.
alleles and the expression of the transcriptional factors [20,21]. The studied core collection is represented by the red kernel triticale lines. Some research studies showed that the PHS resistance of red-seeded wheat is due not only to its linkage to the gene of seed color but also to the influence of the flavonoids in the seed coat on seed dormancy. In our case we assumed that the level of them may also influence the PHS resistance in winter triticale. To determine the level of the flavonoids the seed color has been assessed. As a result, it has been revealed that the studied collection differs in tint of red color of the seed and consists of twenty-five light and twenty-three dark red seeded triticale (Table 1). Thereafter, the strength of the association between the PHS resistance and seed color was estimated using contingency coefficient of Pearson and association coefficient of Yule [19]. As a result, the values of the coefficients did not exceed 0.3 and 0.5, respectively, therefore, the association is not significant.
3.2. The Allelic State of Vp-1B
The STS marker [11] was applied to find out the Vp-1B allelic structure of the core collection. The Vp-1Ba allele has been detected in twenty-four triticale lines (50.0%), the Vp-1Bc allele has been revealed in twenty lines (41.7%), both alleles has been found in four lines (8.3%) (Table 1). No Vp-1Bb, Vp-1Bd, Vp-1Be or Vp-1Bf alleles has been found in the collection.
The influence of the allelic state of Vp-1B on PHS resistance was shown only in white-seeded wheat [11]. By analogy, we estimated the significance of the association between the PHS resistance and allelic state of Vp-1B in the triticale lines with dark red and light red seeds. No statistically significant association has been found in the dark red-seeded lines. At the same time, the association has appeared to be significant in the light red-seeded lines (the contingency coefficient is 0.37 and the association coefficient is 0.67).
4. DISCUSSION
In our study for the first time the association between the PHS resistance, allelic state of Vp-1B and intensity of seed color in winter triticale was analyzed.
It has been revealed that 50.0% of the analyzed triticale lines have Vp-1Bа allele, 41.7% carry the Vp-1Bc allele, 8.3% of the collection possesses both of them (Table 1). The results are in accordance with that obtained in wheat. The alleles Vp-1Bа and Vp-1Bc were shown to be the most widespread in Chinese and European wheat as well [12,14,22,23]. In our collection of winter triticale no Vp-1Bb, Vp-1Bd, Vp-1Be or Vp-1Bf alleles have been found. These alleles appear relatively rare in wheat with the exception for Vp-1Bd in the European collection of wheat [12]. Therefore, in the whole, the allelic structure of the studied triticale collection and different wheat collections are rather similar.
The color of seed in wheat and rye is determined by the accumulation of the phenolic products of the flavonoid pathway. The polyphenolic compounds are shown to be the inhibitors of germination [24,25]. The product of the Vp gene responsible for the embryo sensitivity to ABA, embryo maturation and its transition to dormancy [26] is also known to be involved in the flavonoid pathway [27]. Moreover, flavonoid synthesis genes controlling seed color, Vp-1 and other transcriptional factors of the flavonoid pathway are localized to the distal region of the third homoeological group chromosome in wheat [21,28]. So, the factors determining PHS resistance and seed color in wheat and rye demonstrate tight interaction at biochemical pathways and genetic linkage. The role of the phenolic compounds in caryopsis as PHS resistance regulator in triticale was shown by Weidner et al. (1999) [29]. At the same time, the first studies did not reveal any association between Vp-1 expression and PHS resistance in triticale [30]. The tint of red color of the seed and PHS resistance, shown at our work, in triticale are likely to be a result of multiple interactions between the products of the flavonoid pathways and various transcriptional factors including Vp-1 analogs of wheat and rye genomes. Nevertheless, the association between PHS resistance and Vp-1B allelic state in light red-seeded triticale resembles the same as in white-seeded wheat. This allows suggesting that the similar biochemical and genetic mechanisms conferring PHS resistance are involved in both cases. The result of this mechanism is likely to be the greater contribution of Vp-1B to PHS resistance at the lower level of the compounds determining seed color or/ and inhibition of germination.
In conclusion, the allelic state of Vp-1B has been found to be associated with the PHS resistance in the light red-seeded lines of winter triticale: Vp-1Bc and Vp-1Ba alleles are linked with PHS resistance and susceptibility, respectively. This statement is in agreement with the results obtained on the light red-seeded sister lines F5 (I3) obtained from cross of the resistant and non resistant to PHS triticale lines [16]. At the same time, this association has not been found in the dark redseeded triticale lines. The STS molecular marker for Vp-1B alleles can be applied in marker assisted selection of winter triticale. However, its application can be limited to the lines with light red seeds only.
5. ACKNOWLEDGEMENTS
The work was supported by the Federal Target Program “Researches and developments on priority directions in the development of scientific-technological complex of Russia in 2007-2013”, state contract no. 16.518.11.7089. “The conduction of scientific research with the use of unique devices “The complex for conducting interdisciplinary studies in the sphere of comparative and functional plant genomics”.