Down Regulation of Survivin Gene and Up Regulation of p53 Gene expression by siRNA Induces Apoptosis in human Hepatocellular Carcinoma cell Line HepG2

Yun-Hua Lu, Cong Tang, Wei Wang, Tao Xi

Research Centre of Biotechnology, China Pharmaceutical University, NanJing.
Research Centre of Biotechnology, China Pharmaceutical University, NanJing, 210009, China.

DOI: 10.4236/jbise.2009.21010                

Abstract

Survivin gene may be a good target for cancer gene therapy because it is over expressed in a variety of human tumors including human hepatocellular carcinoma but not in differen- tiated adult tissues. To explore the effects of the siRNA of survivin gene inducing apoptosis in human hepatocellular cancer cells, three siRNAs cpusiRNA1, cpusiRNA2 and cpusiRNA3 were designed and transferred into human hepatocellular carcinoma cell line HepG2 (HepG2) by lipofection. MTT test showed that the growth of HepG2 decreased when it was transfected with 25nM, 50nM, 100nM, 150nM, 200nM, 400nM siRNA respectively after 48 hours. And the change of mRNA and protein of survivin gene and p53 gene had been detected by RT-PCR and Western blot. Cells presented an increase in apoptosis index was assayed by flow cytometry. Small interfering RNA can exert a knockdown of survivin gene expression and up regulation of p53 gene to induce apoptosis and to inhibit the growth of HepG2.

Keywords

RNAi, Survivin gene, p53 gene, Apoptosis, HepG2

Conflicts of Interest

The authors declare no conflicts of interest.

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[17]	Corresponding author; Prof. Xi Tao, Research Centre of Biotech- nology, China Pharmaceutical University, NanJing, 210009, China