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Seroprevalence of Clostridium tetani in Donkeys in Kaduna State, Nigeria

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DOI: 10.4236/ojas.2020.101003    35 Downloads   80 Views  

ABSTRACT

Tetanus is an acute non-contagious and infectious disease caused by Clostridium tetani exotoxins that affect many animal species and humans. It is associated with high mortality rate, ranging from 58% to 80% in Equidae. This study investigated the seroprevalence of C. tetani antibodies in donkeys in Kaduna State. A total of 384 donkeys were sampled from the study area, 5 ml of blood was collected aseptically from the jugular vein and sera was harvested and tested for tetanus using ELISA kits. A seroprevalence of C. tetani of 295/384 (76.8%) was recorded. Male donkeys had a higher sero-prevalence (89.9%) than female (64.1%), young donkeys had 78.5% compared to 75.7% for adults; donkeys with wounds had a seroprevalence of 92.1% while those without wounds (42.4%). Donkeys from free range had a higher seroprevalence of 88.0%. Donkeys with BCS of 1 and 2 had 87.8% being the highest value, based on breeds, the Fari and Idabari had the higher seroprevalence (85.7% and 87.2% respectively), It was concluded that the donkeys in the Northern Kaduna had a high seroprevalence to C. tetani and also sex, age, breeds and presence of wounds were the main risk factors to C. tetani infection in donkeys and it was recommended that the use of donkeys in production of tetanus antitoxins and toxoid should be investigated.

1. Introduction

Equidae is a family of donkeys, horses and zebras. They have various uses ranging from sports to recreation, however, donkeys are mostly used as draught and pack animals because they are intelligent, easy to train and also cheap to maintain [1].

Even as donkeys continue to be a source of livelihood to human lives in arid areas, their physical needs are always ignored due to their hardiness [2].

Tetanus is an acute non contagious infectious disease caused by C. tetani exotoxins that affect many animal species and human beings [3]. The causative agent of tetanus is Clostridium tetani, [4]. Tetanus continues to be associated with a high mortality rate, ranging from 58% to 80% in Equidae [5] [6].

Among animal species, the horse and its family are considered most susceptible to tetanus toxin [7]. The clinical signs of tetanus in animals include spasm of the head muscles, lockjaw, prolapse of the third eyelid [8]. Ears stand erect and the horses can adopt a sawhorse stance in severe case and sudoresis, then recumbency before death [9].

Diagnosis is usually through clinical signs [10]. Treatment is usually through administration of tetanus antitoxin with small amount of toxoid. Penicillin is also administered to help fight the bacterial spores [11]. Prevention is usually through vaccination and thorough wound treatment [12].

Donkeys are money generating power of the equine, a great population of the arid regions depends on it for their source of livelihood, but their health needs have been greatly ignored, and wounds have been known to be of great concern in donkeys even with this, little recorded cases of tetanus in donkeys exist so this study is designed to investigate immune response to C. tetani in donkeys in Northern Kaduna State, Nigeria.

2. Methodology

2.1. Study Area

The study was carried out in Northern part of Kaduna State, because that is the part of the state with high population of donkeys and these donkeys are mostly used for work and transportation. The State lies between longitude 7˚ and 44' East of the Greenwich Meridian and latitude 6˚ and 11' North of the Equator [13]. Kaduna State has distinct wet and dry seasons and it is within the Guinea Savannah vegetation zone and part of the Sudan Savanna of Nigeria. It has an annual rainfall of between 1000 mm and 1500 mm and a rainy season, which is from 120 to 150 days long [13]. It is essentially an agrarian society with about 75% of the population engaging in farming. It also has potentials for the livestock industry [14].

2.2. Physical Examination

Physical examination was conducted, the ages, sex and breed were recorded. Body condition of each donkey was scored according to Pearson scale; as thin (1 and 2), moderate (3 and 4) and fat (5) [15]. The body was examined for the presence or absence of wounds.

Names of breeds of donkeys in Hausa which were according to their colours commonly found in Nigeria are; Auraki (Rust or red colour) Dune Dark brown to black colour) Fari (Pale cream to white colour) and Idabari (Grey to light-medium brown colour) [16].

2.3. Blood Sampling and Serological Investigation

Five milliliters of blood was aseptically collected from the jugular vein of each donkeys. Serum was harvested after centrifuging (at ~375 g for 10 minutes) and serological analysis was performed using i-ELISA kit based on the manufacturer’s instruction.

2.4. Data Analyses

The data obtained were reduced to tables (descriptive statistics) and analyzed using Statistical Package for Social Sciences (SPSS) version 20.0 (SPSS Inc. Chicago IL, USA).

3. Results

Out of 384 donkeys sampled, a seroprevalence of C. tetani of 76.8% was recorded. The seroprevalence of C. tetani was higher in male (89.9%) than female (64.1%). The difference was statistically significant. Young donkeys had a slightly higher seroprevalence of C. tetani of 78.5% while adult donkeys had 75.7% (Table 1). For wound, the seroprevalence of C. tetani was significantly higher in donkeys with wounds (92.1%) than without wounds (42.4%). Function of donkeys, those not used for work had low seroprevalence of C. tetani (45.5%) while those found in the free range and mostly used for work; as pack animals for transportation of goods and farming, had a higher seroprevalence of C. tetani of 88.0% (Table 2). Donkeys with 1 and 2 BCS had the highest seroprevalence of C. tetani (87.8%), while donkeys with BCS of 5 had the lowest seroprevalence (44.4%). For Breed, the Idabari and Fari breeds of donkeys had the highest seroprevalence of C. tetani (87.2% and 85.7%) respectively, while Auraki breed had the lowest seroprevalence of C. tetani (45.9%) (Table 3).

Table 1. Seroprevalence of Clostridium tetani by the sex and age of Donkeys in Northern Kaduna State Nigeria.

P ≤ 0.05 is significant.

Table 2. Seroprevalence of Clostridium tetani based on wounds and purpose of Donkeys in Northern Kaduna State Nigeria.

P ≤ 0.05 is significant.

Table 3. Seroprevalence of Clostridium tetani based on Body Condition Score (BCS) and Breeds of Donkeys in Northern Kaduna State, Nigeria.

P ≤ 0.05 is significant.

4. Discussion

A high seroprevalence was recorded this could be due to the fact that, wounds which are the portal of entry for the causative agent of tetanus was found on donkeys in the study area, the wounds are usually superficial which doesn’t give the organism enough hypoxic environment for toxin production, hence this could be the cause of high seroprevalence, yet little recorded cases of tetanus in donkeys and also immunity could be gotten from low grade infection of the intestine after ingestion of C. tetani contaminated faeces by donkey [17].

Young donkeys had a higher seroprevalence than Adult donkeys this could be due to transmission of antibodies from the dam through suckling, and also the eating habit of young donkeys could involve eating grasses with C tetani infected feaces.

Seroprevalence of C. tetani is higher in male donkeys this could be due to the fact that Male donkeys are more active and thus exposed to more injuries and these injuries serve as a portal for entry of C. tetani which in turn elicit immune response.

Donkeys with wounds had a higher seroprevalence of C. tetani than those without wounds. This could be because wounds are a major risk factor in tetanus; it serves as the main portal of infection of C. tetani and are foci for proliferation and toxin release.

Similarly donkeys sampled from villages i.e. free range had a higher prevalence this is because they are mostly kept for work and most of the villagers don’t usually seek Veterinary care for their donkeys, thus they are exposed to injuries which is a risk factor for C. tetani proliferation whereas the donkeys not use for work; those kept as pet and also for research purposes and are rarely exposed to injuries and diseases.

Donkeys with Body Condition Score of 1 and 2 had the highest Seroprevalence of C. tetani. This could be because BCS of 1 and 2 is characterized by emaciation, presence of sharp keel bones and also wounds at pressure points this could have aided infection of the organism and which then elicit immune response.

The seroprevalence of C. tetani varies across donkey breeds. Fari and Idabari had the highest values because they are stronger among the breeds and as such are exposed to more work and less health care hence prone to injuries [16].

5. Conclusion

A high seroprevalence of C. tetani in donkey was recorded (76.8%) in northern Kaduna State, Sex, age, Wound, Purpose, Body Condition Score and breeds of donkeys all affected the seroprevalence of C. tetani in donkeys in the study area.

Recommendations

The roles of donkeys in the epidemiology of tetanus in animals should be investigated. The use of donkeys as a source of Tetanus Anti Toxin should be studied.

Sources of Funding

No external funding was gotten for the study.

Conflicts of Interest

The authors declare no conflicts of interest regarding the publication of this paper.

Cite this paper

Emmanuel, O. , Mohammed, S. , Mordecai, A. , Abdulrasheed, A. and Deborah, U. (2020) Seroprevalence of Clostridium tetani in Donkeys in Kaduna State, Nigeria. Open Journal of Animal Sciences, 10, 33-39. doi: 10.4236/ojas.2020.101003.

References

[1] Inns, F.M. (1979) Animal Power in Agricultural Production Systems. Agricultural Mechanization Group of Agriculture Services Division, FAO, Iringa, 143 p.
[2] Kataria, N. and Kataria, A.K. (2010) Assessment of Stress due to Hot Ambience in Donkeys from Arid Tracts in India. Journal of Stress Physiology and Biochemistry, 6, 12-17.
[3] Cvetnic, S. (2002) Bacterial and Fungal Diseases of Animal (in Croatia) Medicinska naklada. Zagreb, 71-75.
[4] Slonczewski, J.L. and Foster, J.W. (2010) Microbiology: An Evolving Science. W. W. Norton and Company, Incorporated, Pennsylvania, PA, 1096 p.
[5] Van Galen, G., Delguste, C., Sandersen, C., Verwilghen, D., Grulke, S. and Amory, H. (2008) Tetanus in the Equine Species: A Retrospective Study of 31 Cases. Tijdschrift voor Diergeneeskunde, 133, 512-517.
[6] Reichmann, P., Lisboa, J.A.N. and Araujo, R.G. (2008) Tetanus in Equids: A Review of 76 Cases. Journal of Equine Veterinary Science, 9, 518-523.
https://doi.org/10.1016/j.jevs.2008.07.019
[7] MacKay, R.J. (2007) Tetanus. In: Sellon, D.C. and Long, M.T., Eds., Equine Infectious Diseases, Saunders, St. Louis, 376-380.
https://doi.org/10.1016/B978-1-4160-2406-4.50052-1
[8] Radostits, O.M., Gay, C.C., Blood, D.C. and Hinchcliff, K.W. (2007) Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 10th Edition, Saunders, London, 822-824.
[9] Johnston, J. (1987) Tetanus. In: Robinson, N.E., Ed., Current Therapy in Equine Practice, Saunders, Philadelphia, PA, 370-373.
[10] Kahn, C.M. (2010) The Merck Veterinary Manual. 10th Edition, Merck and Co., Inc., Rahway, N.J., 23-26.
[11] Rosskopf, U., Noeske E. and Werner, E. (2005) Efficacy Demonstration of Tetanus Vaccines by Double Antigen ELISA. Pharmeuropa Biology, 1, 31-52.
[12] Heldens, J., Pouwels, H., Derks, C., Van de Zande, S. and Hoeijmakers, M. (2010) Duration of Immunity Induced by an Equine Influenze and Tetanus Combination Vaccine Formulation Adjuvanted with Iscom-Matrix. Vaccine, 28, 6989-6996.
https://doi.org/10.1016/j.vaccine.2010.08.043
[13] KDSG (Kaduna State Government) (2008) Kaduna State Achievements, In: Data of Estimated Annual Animal Population and Fish Investment Opportunities in Kaduna State, 16-18.
[14] RIM (1992) Nigerian National Livestock Resource Survey. Report by Resource Inventory and Management Limited (RIM) to Federal Department of Livestock and Pest Control Services (FDL&PCS), Abuja, Nigeria, 432 p.
[15] Pearson, R.A. (2005) Body Condition Scores for Donkeys. Produced for Center for Tropical Veterinary Medicine, Division of Animal Health and Welfare, University of Edinburgh, Easter Bush Veterinary Center, Roslin, UK, 27-29.
[16] Blench, R., De Jode, A. and Gherzi, E. (2015) Donkeys in Nigeria: History, Distribution and Productivity. A Resource Book of the Animal Traction Network for Eastern and Souhtern Africa (ATNESA) ACP-EU Technical Centre for Agricultural and Rual Corporation (CTA), Wageningen, The Netherlands, 244 p.
[17] CDC (Centers for Disease Control and Prevention) (2006) “Tetanus” (PDF). In: Atkinson, W., Hamborsky, J., McIntyre, L., et al., Ed., Epidemiology and Prevention of Vaccine-Preventable Diseases (The Pink Book), 10th Edition, Public Health Foundation.

  
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