Rapid and Sensitive Analysis of Eight Polyphenols in Tobacco by Rapid Resolution Liquid Chromatogarphy

Fuwei Xie, Ajuan Yu, Dengke Hou, Huimin Liu, Li Ding, Shusheng Zhang

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DOI: 10.4236/ajac.2011.28107                

Abstract

A rapid resolution liquid chromatographic (RRLC) method for the determination of eight polyphenols in to-bacco was developed. Polyphenols were extracted from tobacco samples by methanol/ water in an ultrasonic bath, then subjected to clean up by solid phase extraction. The separation was performed on a 50 × 4.6 mm, 1.8 μm ZORBAX Eclipse XDB-C18 column. Compared with conventional HPLC method, the analysis time for eight polyphenols by RRLC method was reduced from 20 to 5 min without sacrificing resolution, and the sensitivity was improved. This method appears simple, accurate and precious. The relative standard devia-tions (RSD) of overall analysis procedure for eight tobacco polyphenols were less than 2% with the recover-ies ranging from 94% to 107%. This method could be applied to the rapid determination of major polyphe-nols in tobacco with satisfactory results.

Keywords

Solid Phase Extraction, RRLC, Tobacco, Polyphenols

Conflicts of Interest

The authors declare no conflicts of interest.

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