of chondrocytes that may be affected by the age and stage of maturation of an animal. Further investigations are required to ascertain the stage of musculoskeletal maturation in different species and compare the [Ca2+]i of chondrocytes in mature and immature animals, and also [Ca2+]i from diseased or damaged tissue to those of normal, healthy tissue. The behaviour of Fura-2 could also account for these differences and further studies are required to elucidate this before ovids are used as a large animal model for investigating the physiology or pathophysiology of chondrocytes when access to human tissue is limited.


RW helped plan the experiments, carried them out, analysed the data and prepared the manuscript. JSG planned the experiments and helped write the manuscript. All authors have read and approved the final manuscript. This work was supported by a BBSRC Studentship held by RW. RW received financial support from University Centre Myerscough for the publication of this article.

Declaration of Conflicting Interests

The Author(s) declare that there are no conflicts of interests.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Yellowley, C.E., Jacobs, C.R., Li, Z.H., Zhou, Z. and Donahue, H.J. (1997) Effects of Fluid Flow on Intracellular Calcium in Bovine Articular Chondrocytes. American Journal of Physiology, 273, C30-C36.
[2] Browning, J., Walker, R., Hall, A. and Wilkins, R. (1999) Modulation of Na+ x H+ Exchange by Hydrostatic Pressure in Isolated Bovine Articular Chondrocytes. Acta Physiologica Scandinavica, 166, 39-45.
[3] Guilak, F., Zell, R.A., Erickson, G.R., Grande, D.A., Rubin, C.T., McLeod, K.J. and Donahue, H.J. (1999) Mechanically Induced Calcium Waves in Articular Chondrocytes Are Inhibited by Gadolinium and Amiloride. Journal of Orthopaedic Research, 17, 421-429.
[4] Yellowley, C.E., Hancox, J.C. and Donahue, H.J. (2002) Effects of Cell Swelling on Intracellular Calcium and Membrane Currents in Bovine Articular Chondrocytes. Journal of Cell Biochemistry, 86, 290-301.
[5] Endres, M., Neumann, K., Zhou, B., Freymann, U., Pretzel, D., Stoffel, M., Kinne, R.W. and Kaps, C. (2012) An Ovine in Vitro Model for Chondrocyte-Based Scaffold-Assisted Cartilage Grafts. Journal of Orthopaedic Surgery and Research, 7, 1.
[6] Garcia, D., Giuseppe Longo, U., Vaquero, J., Forriol, F., Loppini, M.S., Khan, W. and Denaro, V. (2015) Amniotic Membrane Transplant for Articular Cartilage Repair: An Experimental Study in sHeep. Current Stem Cell Research Therapy, 10, 77-83.
[7] Power, J., Hernandez, P., Guehring, H., Getgood, A. and Henson, F. (2014) Intra-Articular Injection of rhFGF-18 Improves the Healing in Microfracture Treated Chondral Defects in an Ovine Model. Journal of Orthopaedic Research, 32, 669-676.
[8] Raven, E.T. (1989) Cattle Footcare and Claw Trimming. Diamond Farm Book Pubns, Brighton.
[9] Bokko, B.P. and Chaudhari, S.U.R. (2001) Prevalence of Lameness in Sheep in the North East Region of Nigeria. International Journal of Agricultural Biology, 3, 519-521.
[10] Neveux, S., Weary, D.M., Rushen, J., Von Keyserlingk, M.A. and De Passillé, A.M. (2006) Hoof Discomfort Changes How Dairy Cattle Distribute Their Body Weight. Journal of Dairy Science, 89, 2503-2509.
[11] van der Tol, R., Somers, J., Weijs, W. and Stassen, E. (2006) Lameness in Cattle: Are We on the Wrong Track? Veterinary Sciences Tomorrow, 2006, 1-8.
[12] Martel-Pelletier, J., Boileau, C., Pelletier, J.P. and Roughly, P.J. (2008) Cartilage in Normal and Osteoarthritis Conditions. Best Practice & Research: Clinical Rheumatology, 22, 351-384.
[13] Wilkins, R.J. and Hall, A.C. (1995) Control of Matrix Synthesis in Isolated Bovine Chondrocytes by Extracellular and Intracellular pH. Journal of Cell Physiology, 164, 474-481. https://doi.org/10.1002/jcp.1041640305
[14] Sanchez, J.C. and Lopez-Zapata, D.F. (2015) Effects of Adipokines and Insulin on Intracellular pH, Calcium Concentration, and Responses to Hypo-Osmolarity in Human Articular Chondrocytes from Healthy and Osteoarthritic Cartilage. Cartilage, 6, 45-54.
[15] Browning, J.A. and Wilkins, R.J. (1998) The Characterisation of Mechanisms Regulating Intracellular pH in a Transformed Human Articular Chondrocyte Cell Line C-20/A4. Journal of Physiology P, 513, 54.
[16] O’Neill, W.C. (1999) Physiological Significance of Volume-Regulatory Transporters. American Journal of Physiology, 276, C995-C1011.
[17] Sanchez, J. and Wilkins, C.R.J. (2003) Effects of Hypotonic Shock on Intracellular pH in Bovine Articular Chondrocytes. Comparative Biochemistry and Physiology A, 135, 575-583.
[18] Sanchez, J.C. and Wilkins, C.R.J. (2003) Mechanisms Involved in the Increase in Intracellular Calcium Following Hypotonic Shock in Bovine Articular Chondrocytes. General Physiology and Biophysics, 22, 487-450.
[19] Wilkins, R.J., Fairfax, T., Davies, M.E., Muzyamba, M.C. and Gibson, J.S. (2003) Homeostasis of Intracellular Ca2+ in Equine Chondrocytes: Response to Hypotonic Shock. Equine Veterinary Journal, 35, 439-443.
[20] Hall, A.C., Horwitz, E.R. and Wilkins, R.J. (1996) The Cellular Physiology of Articular Cartilage. Exercise Physiology, 81, 435-545.
[21] White, R. and Gibson, J.S. (2010) The Effect of Oxygen Tension on Calcium Homeostasis in Bovine Articular Chondrocytes. Journal of Orthopaedic Surgery and Research, 5, 1-7.
[22] Simon, W.H. (1970) Scale Effects in Animal Joints. 1. Articular Cartilage Thickness and Compressive Strain. Arthritis and Rheumatism, 13, 244-255.
[23] Schmidt-Nielsen, K. (1990) Animal Physiology. Cambridge University Press, Cambridge.
[24] White, R. and Gibson, J.S. (2017) Calcium Homeostasis in Articular Chondrocytes of Two Different Animal Species. Osteoarthritis and Cartilage, 25, S154.
[25] Grynkiewicz, G., Poenie, M. and Tsien, R.Y. (1985) A New Generation of Ca2+ Indicators with Greatly Improved Fluorescence Properties. Journal of Biological Chemistry, 260, 3440-3450.
[26] Williams, D.A. and Fay, F.S. (1990) Intracellular Calibration of the Fluorescent Calcium Indicator Fura-2. Cell Calcium, 11, 75-83.
[27] Kilborn, S.H., Trudel, G. and Uhthoff, H. (2002) Review of Growth Plate Closure Compared with Age at Sexual Maturity and Lifespan in Laboratory Animals. Contempary Topics in Laboratory Animal Science, 41, 21-26.
[28] Brommer, H., Brama, P.A., Laasanen, M.S., Helminen, H.J., Weeren, P.V. and Jurvelin, J.S. (2005) Functional Adaptation of Articular Cartilage from Birth to Maturity under the Influence of Loading: A Biomechanical Analysis. Equine Veterinary Journal, 37, 148-154.
[29] O’Conor, C.J., Leddy, H.A., Benefield, H.C., Liedtke, W.B. and Guilak, F. (2014) TRPV4-Mediated Mechanotransduction Regulates the Metabolic Response of Chondrocytes to Dynamic Load-ing. Proceedings of the National Academy of Sciences, 111, 1316-1321.
[30] Browning, J.A. and Wilkins, R.J. (2002) The Effect of Intracellular Alkalinisation on Intracellular Ca2+ Homeostasis in a Human Chondrocyte Cell Line. European Journal of Physiology, 444, 744-751.
[31] Webber, R.J., Malemud, C.J. and Sokoloff, L. (1977) Species Differences in Cell Culture of Mammalian Articular Chondrocytes. Calcified Tissue Research, 23, 61-66.
[32] Bayliss, M.T., Howat, S., Davidson, C. and Dudhia, J. (2000) The Organization of Aggrecan in Human Articular Cartilage. Journal of Biological Chemistry, 275, 6321-6327. https://doi.org/10.1074/jbc.275.9.6321
[33] Giannoni, P., Crovace, A., Malpeli, M., Maggi, E., Arbico, R., Cancedda, R. and Dozin, B. (2005) Species Variability in the Differentiation Potential of in Vitro-Expanded Articular Chondrocytes Restricts Predictive Studies on Cartilage Repair Using Animal Models. Journal of Tissue Engineering, 11, 237-248.
[34] Konishi, M. (1998) Cytoplasmic Free Concentrations of Ca2+ and Mg2+ in Skeletal Muscle Fibers at Rest and during Contraction. Japanese Journal of Physiology, 48, 421-438.
[35] Oliver, A.E., Baker, G.A., Fugate, R.D., Tablin, F. and Crowe, J.H. (2000) Effects of Temperature on Calcium-Sensitive Fluorescent Probes. Biophysical Journal, 78, 2116-2126.
[36] Uto, A., Arai, H. and Ogawa, Y. (1991) Reassessment of Fura-2 and the Ratio Method for Determination of Intracellular Ca2+ Concentrations. Cell Calcium, 12, 29-37.
[37] Henke, W., Cetinsoy, C., Jung, K. and Loening, S. (1996) Nonmhyperbolic Calcium Calibration Curve of Fura-2: Implications for the Reliability of Quantitative Ca2+ Measurements. Cell Calcium, 20, 287-292.
[38] Jiang, Y. and Julian, F.J. (1997) Pacing Rate, Halothane, and BDM Affect Fura 2 Reporting of [Ca2+]i in Intact Rat Trabeculae. American Journal of Physiology, 273, C2046-C2056.
[39] Brenowitz, S.D. and Regeh, W.G. (2002) Calcium Dependence of Retrograde Inhibition by Endocannabinoids at Synapses onto Purkinje Cells. Journal of Neuroscience, 23, 6373-6384.
[40] Palmer, B.M. and Moore, R.L. (2000) Excitation Wavelengths for Fura 2 Provide a Linear Relationship between [Ca(2+)] and Fluorescence Ratio. American Journal of Physiology—Cell Physiology, 279, C1278-C1284.
[41] Gomes, P.A., Bassani, R.A. and Bassani, J.M. (1998) Measuring [Ca2+] with Fluorescent Indicators: Theoretical Approach to the Ratio Method. Cell Calcium, 24, 17-26.
[42] Thomas, D., Tovey, S.C., Collins, T.J., Bootman, M.D., Berridge, M.J. and Lipp, P. (2000) A Comparison of Fluorescent Ca2+ Indicator Properties and Their Use in Measuring Elementary and Global Ca2+ Signals. Cell Calcium, 28, 213-223.

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