Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B


Context: The in-house techniques or experimental methods are increasingly recommended for their low-cost reagents for the determination of the Viral Load (VL) in resource-limited settings. The objective of this study was to compare the determination of VL from HIV-1 non-B samples by an in-house technique with the COBAS AmpliPrep/TaqMan version 2.0. Method: In this cross-sectional study, 39 plasma samples from patients infected with HIV type 1 non-B from N’Djamena and Kinshasa were used to determine the VL using the two techniques. Results: The mean values of VL are respectively 4.68 ± 1.26 and 4.58 ± 1.33 log10 RNA copies/ml for the COBAS AmpliPrep/TaqMan assays and the in-house assays. A good correlation (Spearman Correlation) was obtained, with a coefficient (R2) of 0.9452. Conclusion: This study demonstrates that there is no significant difference between the results of VL determined by the COBAS AmpliPrep/TaqMan assays and the in-house assays used.

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Kamangu, E. , Chatte, A. , Boreux, R. , Susin, F. , Kalala, R. , Mvumbi, G. , Mol, P. , Hayette, M. and Vaira, D. (2015) Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B. Open Access Library Journal, 2, 1-7. doi: 10.4236/oalib.1101402.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Department of Health and Human Services (DHHS) (2011) Guidelines for the Use of Antiretroviral Agents in HIV-Infected Adults and Adolescents. US Government Printing Office, Washington DC, 174.
[2] World Health Organization (WHO) (2006) Towards Universal Access by 2010. How WHO Is Working with Countries to Scale-Up HIV Prevention, Treatment, Care and Support. World Health Organization, Geneva.
[3] World Health Organization (WHO) (2010) Antiretroviral Therapy for HIV Infection in Adults and Adolescents: Recommendations for a Public Health Approach (2010 Revision). World Health Organization, Geneva.
[4] Thompson, M.A., Aberg, J.A., Cahn, P., Montaner, J.S., Rizzardini, G., Telenti, A., Gatell, J.M., Gunthard, H.F., Hammer, S.M., Hirsch, M.S., Jacobsen, D.M., Reiss, P., Richman, D.D., Volberding, P.A., Yeni, P. and Schooley, R.T. (2010) Antiretroviral Treatment of Adult HIV Infection: 2010 Recommendations of the International AIDS Society-USA Panel. JAMA, 304, 321-333.
[5] Steegen, K., Luchters, S.S., De Cabooter, N., Reynaerts, J., Mandaliya, K., Plum, J., Jaoko, W., Verhofstede, C. and Temmerman, M. (2007) Evaluation of Two Commercially Available Alternatives for HIV-1 Viral Load Testing in Resource-Limited Setting. Journal of Virological Methods, 146, 178-187.
[6] Rouet, F. and Rouzioux, C. (2007) The Measurement of HIV-1 Viral Load in Resource-Limited Setting: How and Where? Clinical Laboratory, 53, 135-148.
[7] Rouet, F., Chaix, M.L., Nerrienet, E., Ngo-Giang-Huong, N., Plantier, J.C., Burgard, M., Peeters, M., Damond, F., Koumavi, E.D., Msellati, P., Ferradini, L., Rukobo, S., Maréchal, V., Schvachsa, N., Wakrim, L., Rafalimanana, C., Rakotoambibiba, B., Viard, J.P., Seigneurin, J.M., Rouzioux, C. and for the Agence Nationale de Recherchessur le SIDA AC11/AC12 Working Groups (2007) Impact of HIV-1 Genetic Diversity on Plasma HIV-1 RNA Quantification. Journal of Acquired Immune Deficiency Syndromes, 45, 380-388.
[8] Rouet, F., Foulongne, V., Viljoen, J., Steegen, K., Becquart, P., Valéa, D., Danaviah, S., Segondy, M., Verhofstede, C., Van de Perre, P. and for the WHO/ANRS 1289 Kesho Bora Study Group (2010) Comparison of the Generic HIV Viral Load® Assay with AmplicorTM HIV-1 Monitoring v1.5 and Nuclisens HIV-1 EasyQ® v1.2 Techniques for Plasma HIV-1 RNA Quantification of Non-B Subtypes: The Kesho Bora Preparatory Study. Journal of Virological Methods, 163, 253-257.
[9] Drosten, C., Panning, M., Drexler, J.F., Hänsel, F., Pedrosoc, C., Yeats, J., de Souza Luna, L.K., Samuel, M., Liedigk, B., Lippert, U., Stürmer, M., Doerr, H.W., Brites, C. and Preiser, W. (2006) Ultrasensitive Monitoring of HIV-1 Viral Load by a Low-Cost Real-Time Reverse Transcription-PCR Assay with Internal Control for 5’ Long Terminal Repeat Domain. Clinical Chemistry, 52, 1258-1266.
[10] QIAGEN (2010) QIAamp® RNA Mini and Blood Mini Handbook. 3rd Edition, 27-29.
[11] Steegen, K., Demecheleer, E., De Cabooter, N., Nges, D., Temmerman, M., Ndumbe, P., Mandaliya, K., Plum, J. and Verhofstede, C. (2006) A Sensitive In-House RT-PCR Genotyping System for Combined Detection of Plasma HIV-1 and Assessment of Drug Resistance. Journal of Virological Methods, 133, 137-145.
[12] Adawaye, C., Kamangu, N.E., Moussa, A.M., Tchombou, H.Z.B., Vaira, D. and Moutshen, M. (2015) Prévalence de la résistance acquise et diversité génétique des souches du VIH-1 circulant à Ndjamena au Tchad. In Press.
[13] Kamangu, N.E., Adawaye, C., Susin, F., Boreux, R., Kalala, L.R., Mvumbi, L.G., De Mol, P., Vaira, D. and Hayette, M.P. (2015) Genetic Diversity and Antiretroviral Drug Resistance among Drug-Naïve HIV Type 1 Infected Patients Attending Clinics in Kinshasa, Democratic Republic of Congo. In Press.
[14] Tang, M.W., Liu, T.F. and Shafer, R.W. (2012) The HIVdb System for HIV-1 Genotypic Resistance Interpretation. Intervirology, 55, 98-101.
[15] Viard, J.P. (2008) Surveillance biologique du patient sous traitement antiretroviral. Revue Francophone des Laboratoires, 399, 22-24.
[16] Thomson, M.M. and Najera, R. (2005) Molecular Epidemiology of HIV-1 Variants in the Global AIDS Pandemic: An Update. AIDS Reviews, 7, 210-224.
[17] Shao, Y. and Williamson, C. (2012) The HIV-1 Epidemic: Low-to Middle-Income Countries. Cold Spring Harbor Perspectives in Medicine, 2, a007187.
[18] Kamangu, N.E., Kabututu, Z., Mvumbi, L.G., Kalala, L.R. and Mesia, K.G. (2013) Genetic Diversity of Human Immunodeficiency Virus Type 1 in the Democratic Republic of Congo. International Journal of Collaborative Research on Internal Medicine & Public Health (IJCRIMPH), 5, 295-309.
[19] Kamangu, N.E., Kalala, N.H. and Mesia, K.G. (2012) Profile of Antiretroviral Treatment Centers in Kinshasa, Democratic Republic of Congo [Poster 388]. Proceedings of the 1st International African Society of Laboratory Medicine (ASLM) Conference, Cape Town, 1-7 December 2012, 377.

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