A new approach for reduction of methylene green withascorbic acid by de-oxygenation through carbondioxide
Rafia Azmat, Noshab Qamar, Raheela Naz
DOI: 10.4236/ns.2011.37079   PDF   HTML     6,096 Downloads   11,650 Views   Citations


Reduction kinetics of the methylene green (MG) with ascorbic acid (AA) in acidic medium at λmax 660 nm was monitored through visible spectrophotomtry in absence and presence of sodium carbonate. CO2 release through reaction of sodium carbonate and oxalic acid, created deoxygenated atmosphere for reduction of dye which greatly boosted the reaction rate. Initially slow reaction in presence of atmospheric oxygen proceeded rapidly when sodium carbonate was added. The reaction followed fractional order kinetics with AA and zero order kinetics with MG. The rate of reaction shows no linear dependence on [H+] concentration as an acidic medium. The rate of reaction is directly related with the elevated concentration of salt, which suggests that the two same charged species are involved in the rate determining step. Secondary reactions at elevated temperature showed complex kinetics.

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Azmat, R. , Qamar, N. and Naz, R. (2011) A new approach for reduction of methylene green withascorbic acid by de-oxygenation through carbondioxide. Natural Science, 3, 566-572. doi: 10.4236/ns.2011.37079.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Kishore, K.T. (2010) A new spectrophotometric method for the determination of ascorbic acid using leuco malachite green. Journal of the Chinese Chemical Society, 57, 105-110.
[2] Massoud, N.-Y. (2007) Indirect determination of ascorbic acid (vitamin C) by spectrophotometric method. International Journal of Food Science & Technology, 42, 1402- 1407.
[3] Cooper, J.A., Woodhouse, K.E., Chippindale, A.M. and Compton, R.G. (1999) Photo electrochemical determination of ascorbic acid using methylene blue immobilized in α-zirconium phosphate. Electroanalysis, 11 1259-1265. doi:10.1002/(SICI)1521-4109(199911)11:17<1259::AID-ELAN1259>3.0.CO;2-B
[4] Strizhak, P.E. (1995) Effect of copper(II) ions on kinetics of ascorbic acid oxidation by methylene blue. Theoretical and Experimental Chemistry, 30, 239-244. doi:10.1007/BF00536697
[5] Strizhak, P.E. (1994) Kinetics of oxidation of ascorbic acid by methylene blue in acid solutions. Theoretical and Experimental Chemistry, 29, 283-286. doi:10.1007/BF00531462
[6] Yusuf, D. and Giirel, N. (2006) Flow injection photoamperometric investigation of ascorbic acid using methylene blue immobilized on titanium phosphate. Analytical letters, 39, 451-465. doi:10.1080/00032710500536053
[7] Sultan, S.M., Abdennabi, A.M. and Suliman, F.E.O. (1994) Flow injection colorimetric method for the assay of Vitamin C in drug formulation using tris 1-10 phenanthroline iron complex as an oxidant in sulphuric acid media. Talanta, 41, 125. doi:10.1016/0039-9140(94)80177-0
[8] Tewari, B.B. (2005) Dehydrogenation studies of ascorbic acid with methylene blue in the presence of copper hexacyanoferrate(II) complex and light. Bulletin of the Che- mists and Technologists of Macedonia, 24, 109-115.
[9] Cooper, J.A., Wu, M. and Compton, R.G. (1998) Photoelectrochemical analysis of ascorbic acid. Analytical Che- mistry, 70, 2922-2927. doi:10.1021/ac980123q
[10] Verma P., et al. (2003) Decolorization of structurally different synthetic dyes using cobalt(II)/ascorbic acid/ hydrogen peroxide system. Chemosphere, 50, 975-979. doi:10.1016/S0045-6535(02)00705-1
[11] Azmat, R.S., Ahmed, S., et al. (2006) Aerobic oxidation of D-glucose by methylene green in alkaline aqueous solution by visible spectrophotometry. Journal of Applied Polymer Science, 6, 2784-2788.
[12] Azmat, R., Yasmeen, B. and Uddin, F. (2007) Kinetics of methylene blue reduction with oxalic acid by visible spe- ctrophotometric method. Asian Journal of Chemistry, 19, 1115-1121.
[13] Jonnalagadda, S.B. and Dumba, M. (1993) Reduction of toluidine blue by stannous ion at low pH: Kinetics and simulation. International Journal of Chemical Kinetics, 25, 745. doi:10.1002/kin.550250905
[14] Ahmed, K., Uddin, F. and Azmat, R. (2009) Reduction kinetics of thionine in aerobic condition with D-galactose. Chin. Journal of Chemical Physics, 27, 1232-1236. doi:10.1002/cjoc.200990206
[15] Azmat, R. (2009) Reduction of methylene blue with reducing sugars. Publisher VDM Verlag Dr. Müller e.K.
[16] Gest, H. and Stokes, J.L. (1952) The effect of carbon dioxide on reduction of methylene blue by microorganisms. Antonie van Leeuwenhoek, 18, 55-62. doi:10.1007/BF02538590
[17] Bujdák, J. and Nobuo, I. (2002) Visible spectroscopy of cationic dyes in dispersions with reduced-charge montmorillonites. Clays and Clay Minerals, 50, 446-454. doi:10.1346/000986002320514172
[18] Rauf, M.A., Marzouki, N. and K?rbahti, B.K. (2008) Photolytic decolorization of rose bengal by UV/H2O2 and data optimization using response surface method. Journal of Hazardous Materials, 159, 602-609. doi:10.1016/j.jhazmat.2008.02.098
[19] Azmat, R. and F. Uddin (2009) Photo decoloration of methylene blue with ribose at optimum condition by visible radiation. Chinese Journal of Chemistry, 27, 1237- 1243. doi:10.1002/cjoc.200990207
[20] Azmat, R., Qamer, N. and Saeed, A. and F. Uddin (2008) Reduction of Methylene green by EDTA: Kinetics and thermodynamics aspects. Chinese Journal of Chemistry, 26, 631-634. doi:10.1002/cjoc.200890119
[21] Joaquim, A.N. and Fábio, R.P. (1997) Rocha ionic strength effect on the rate of reduction of hexacyanoferrate(III) by ascorbic acid. Journal of Chemical Education, 74, 560-562
[22] Fahim Uddin: Kinetics of Photochemical Reactions of Thionine with Thiourea. Europ. J. Org. Chem., 7 (2000) 1345-1351. doi:10.1002/1099-0690(200004)2000:7<1345::AID-EJOC1345>3.0.CO;2-0
[23] Azmat, R. and Uddin, F. (2008) Photo bleaching of Methylene Blue with Galactose and D-mannose by high intensity radiations. Canadian Journal of Pure and Applied Sciences, 2, 275-283.
[24] Uddin, F. and Hasnain, Q.Z. (2006) Photo reduction of thionine by monomethylamine in 50% aqueous methanol. Pure and Applied Chemistry, 1, 597-605.
[25] Uddin, F., Adhami, I.M. and Yousufzai, M.A.K. (1998) Photochemical reduction of methylene blue by triethylamine. Journal of Saudi Chemical Society, 2, 47-59.
[26] Uddin F., Hasnain, Q.Z. and Yousufzai, M.A.K. (2001) Photo-reduction of thiazine dye with trimethylamine. Arabian Journal for Science and Engineering, 26, 109- 125.
[27] Fahim U. (1996) Photochemical reduction of thionine by N-phenylglycine in methanol. Arab. Arabian Journal for Science and Engineering, 21, 407-424.
[28] Ahmed, T., Uddin, F. and Azmat, R. (2010) Kinetics and mechanistic study of chemical treatment of methylene green by urea. Chinese Journal of Chemistry, 28, 748- 754.
[29] Galagan, Y. and Sua, W.-F. (2008) Reversible photoreduction of methylene blue in acrylate mediacontaining benzyl dimethyl ketal. Journal of Photochemistry and Photobiology A: Chemistry, 195, 378-383. doi:10.1016/j.jphotochem.2007.11.005

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