Influence of Saliva and Mucin on the Adhesion of Candida Oral Clinical Isolates
Catarina L. Seabra1,2,3,4, Cláudia M. Botelho5*, Ana C. N. Oliveira6,7, Mariana Henriques5
1Instituto de investigação e Inovação em Saúde, University of Porto, Porto, Portugal.
2Instituto de Engenharia Biomédica, University of Porto, Porto, Portugal.
3Instituto de Patologia e Imunologia Molecular da Universidade do Porto, University of Porto, Porto, Portugal.
4Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Porto, Portugal.
5Centre of Biological Engineering (CEB), Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Braga, Portugal.
6Centre of Physics (CFUM), Department of Physics, University of Minho, Braga, Portugal.
7Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
DOI: 10.4236/jeas.2015.54018   PDF   HTML   XML   3,565 Downloads   3,958 Views  


Objectives: This research work intends to clarify the role of artificial saliva, in particularly the role of mucin, a salivary protein, on the surface properties and adhesion ability of Candida spp. oral clinical isolates to abiotic surfaces. Methods: Four oral clinical isolates of Candida spp. were used: two Candida albicans strains (AC; AM) and two Candida parapsilosis strains (AD; AM2). The strains were isolated from patients using oral prosthesis. The microorganisms were cultured in the absence or presence of mucin and artificial saliva, and their adhesion to an abiotic surface (coated with mucin and artificial saliva) was evaluated. Results: The presence of mucin per se onto the abiotic surface decreased the adhesion of all strains, although the combination of mucin with artificial saliva had reduced this effect. No direct correlation between adhesion and the surface free energies of adhesion of the microorganisms was found. Significance: Candida spp. were human commensal microorganisms that became pathogenic when the host immune defenses were compromised. Medical devices were colonized by Candida spp. particularly, oral prostheses, which might lead to the degradation of the prostheses and systemic infections. The salivary secretions that constantly cover the oral cavity influenced Candida spp. adhesion process. Therefore, it was important to understand the interactions between Candida spp., salivary proteins and the characteristic of oral prosthesis when developing materials for oral prostheses.

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Seabra, C. , Botelho, C. , Oliveira, A. and Henriques, M. (2015) Influence of Saliva and Mucin on the Adhesion of Candida Oral Clinical Isolates. Journal of Encapsulation and Adsorption Sciences, 5, 217-227. doi: 10.4236/jeas.2015.54018.

Conflicts of Interest

The authors declare no conflicts of interest.


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