Change of Erythrocytes Transport Function in Blood of Men with Prostate Adenocarcinoma (before and after Plastic Orchiectomy)


The changes of blood erythrocytes transport function in patients with prostate adenocarcinoma have been studied according to alterations in Na+/K+-ATP-ase activity and permeability of Na+ and K+ ions, before and after the plastic orchiectomy. The results of the study revealed that activity of Na+/K+-ATP-ase was increased compared with the data before the orchiectomy, but this parameter was reduced compared with control group. The increased amounts of the Na+ as well as K+ ions were also observed in the extracellular area compared with the same data before the orchiectomy. The amount of cholesterol, after plastic orchiectomy, was reduced compared with the data gained before orchiectomy, but the data were greater than those of the control group. It may be presumed that one of the reasons for change of Na+/K+-ATP-ase activity is variation of cholesterol content in erythrocyte membrane. Changes of the active transport system, in turn, affect the transport function of the erythrocyte membrane. In spite of surgical intervention, full recovery of patients does not take place after the plastic orchiectomy. Comparative normalization and approaching to the control group of the given indices in post-operation period (~6 months after surgery) indicate strengthening of the immune system and correspondinglyprotective abilities of an organism, which was proved by the anamneses of patients.

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Alibegashvili, M. , Veshapidze, N. , Ramishvili, L. , Gordeziani, M. , Chigogidze, T. , Gabunia, N. , Khazaradze, A. and Kotrikadze, N. (2015) Change of Erythrocytes Transport Function in Blood of Men with Prostate Adenocarcinoma (before and after Plastic Orchiectomy). Journal of Cancer Therapy, 6, 1249-1253. doi: 10.4236/jct.2015.615136.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Pratap, P.R., Dediu, O. and Nienhaus, G.U. (2003) FTIR Study of ATP-Induced Changes in Na+/K+-ATPase from Duck Supraorbital Glands. Biophysical Journal, 85, 3707-3717.
[2] Xie, Z. and Cai, T. (2003) Na+/-K+-ATP-asa-Mediated Signal Transduction: From Protein Interaction to Cellular Function. Molecular Interventions, 3, 157-168.
[3] Yoon, T., Kim, M. and Lee, K. (2006) Inhibition of Na,K-ATPase-Suppressive Activity of Translationally Controlled Tumor Protein by Sorting Nexin 6. FEBS Letters, 580, 3558-3564.
[4] Pressley, T.A. (1992) Ionic Regulation of Na+/-K+-ATP-ase Expression. Seminars in Nephrology, 12, 67-71.
[5] Staines, H.M., Ellory, J.C. and Kirk, K. (2001) Perturbation of the Pump-Leak Balance for Na and K in Malaria-Infected Erythrocytes. Journal of Cellular Physiology, 280, 1576-1587.
[6] Alevizopoulos, K., Calogeropoulou, T., Lang, F. and Stournaras, C. (2014) Na+/K+ ATPase Inhibitors in Cancer. Current Drug Targets, 15, 988-1000.
[7] Riba, L.W. (1942) Subcapsular Castration for Carcinoma of Prostate. Journal of Urology, 48, 384-387.
[8] Gingrich, J.R., Barrios, R.J. and Kattan, M.W. (1997) Androgen-Independent Prostate Cancer Progression in the TRAMP Model. Journal of Cancer Research, 57, 4687-4691.
[9] Schulz, W.A., Burchardt, M. and Cronauer, M.V. (2003) Molecular Biology of Prostate Cancer. Molecular Human Reproduction, 9, 437-448.
[10] Dodge, J.T., Mitchell, C. and Hanahan, D.J. (1963) The Preparation and Chemical Characteristics of Hemoglobin-Free Ghosts of Human Erythrocytes. Archives of Biochemistry and Biophysics, 100, 119-130.
[11] Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein Measurement with the Folin Phenol Reagent. The Journal of Biological Chemistry, 193, 265-275.
[12] Stolz, M., Lewitzki, E., Mantele, W., Barth, A. and Grell, E. (2006) Inhibition and Partial Reactions of Na,K-ATPase Studied by Fourier Transform Infrared Difference Spectroscopy. Biopolymers, 82, 368-372.
[13] Lishko, V., Malyshev, M. and Grevizyrskaya, T. (1974) The Study of Membrane Na,K-ATPase and Erythrocytes’ Ghosts Interactions by Ouabain. The Journal of Biochemistry, 39, 60-66. (In Russian)
[14] Filipovich, U.B., Egorova, T.A. and Sevastianov, G.A. (1975) General Biochemistry Practical. p. 278.
[15] Alibegashvili, M. (2003) Investigation of Membrane Regulation Changes in Blood Erythrocytes during Tumor Growth (Prostate Benign Hyperplasia, Prostate Adenocarcinoma). PhD Thesis, Tbilisi.
[16] McFarland, R., Zanjani, H.S., Mariani, J. and Vogel, M.W. (2014) Changes in the Distribution of the α3 Na+/K+ ATPase Subunit in Heterozygous Lurcher Purkinje Cells as a Genetic Model of Chronic Depolarization during Development. International Journal of Cell Biology, 2014, Article ID: 152645.
[17] Pchejetski, D., Taurin, S., Der Sarkissian, S., Lopina, O.D., Pshezhetsky, A.V., Tremblay, J., et al. (2003) Inhibition of Na+,K+-ATPase by Ouabain Triggers Epithelial Cell Death Independently of Inversion of the [Na+]i/[K+]i Ratio. Biochemical and Biophysical Research Communications, 301, 735-744.
[18] Kolanjiappan, K., Manoharan, S. and Kayalvizhi, M. (2002) Measurement of Erythrocyte Lipids, Lipid Peroxidation, Antioxidants and Osmotic Fragility in Cervical Cancer Patients. Clinica Chimica Acta, 326, 143-149.
[19] Mangialavori, I., Montes, M.R., Rossi, R.C., Fedosova, N.U. and Rossi, J.P. (2011) Dynamic Lipid-Protein Stoichiometry on E1 and E2 Conformations of the Na+/K+-ATPase. FEBS Letters, 585, 1153-1157.
[20] Calderón Guzmán, D., Hernández García, E., Juárez Jacobo, A., Segura Abarca, L., Barragán Mejía, G., Rodríguez Pérez, R., et al. (2011) Effect of Orlistat on Lipid Peroxidation, Na+, K+ ATPase, Glutathione and Serotonin in Rat Brain. Proceedings of the Western Pharmacology Society, 54, 73-77.
[21] Kotrikadze, N., Alibegashvili, M., Zibzibadze, M., Abashidze, N., Chigogidze, T., et al. (2008) Activity and Content of Antioxidant Enzymes in Prostate Tumors. Experimental Oncology, 30, 244-247.
[22] Kotrikadze, N., Veshapidze, N., Ramishvili, L., Alibegashvili, M., Gordeziani, M., et al. (2014) Prognostic Value of Structural and Ultra-Structural Changes of Erythrocytes. Urology, 84, S291.

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