Isolation and Screening of Filamentous Fungi Producing Extracellular Lipase with Potential in Biodiesel Production
Sabrina Moro Villela Pacheco1, Américo Cruz Júnior1, Ayres Ferreira Morgado1, Agenor Furigo Júnior1, Onyetugo Chioma Amadi2, José Manuel Guisán3, Benevides Pessela4,5*
1Department of Food and Chemistry Engineering, University Campus UFSC, Florianópolis, Brazil.
2Department of Microbiology, Faculty of Biological Science, University of Nigeria, Enugu, Nigeria.
3Departamento de Biocatálises Enzimática, Instituto de Catalisis y Petroleoquimica, ICP (CSIC-UAM), C/Marie Curie 2, Cantoblanco, Campus de la Univerisdad Autónoma de Madrid, Madrid, España.
4Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), C/Nicolás Cabrera 9, Cantoblanco, Campus de la Universidad Autónoma de Madrid, Madrid, España.
5Departamento de Engenharia e Tecnologías, Instituto Superior Politécnico de Tecnología e de Ciências, ISPTEC, Luanda Sul, Talatona, República de Angola.
DOI: 10.4236/aer.2015.34011   PDF   HTML   XML   4,132 Downloads   5,440 Views   Citations


Nineteen fungal strains were isolated from a chicken slaughterhouse effluent and within those, only one showed high values of lipolytic activity in submerged cultures. This fungus was identified as Trametes hirsuta. The crude extract was immobilized in chitosan/clay beads, with an immobilization yield of 80.9%. The analyses of the crude extract and the immobilized derivative at different temperatures, pH (s), solvents, metallic ions and storage showed that the immobilization process increased the enzyme life span. Ethyl esters were obtained in solvent free systems using chicken viscera oil and the enzyme crude extract. For effective comparison, a reaction using viscera oil and commercial lipase Novozym 435 was carried out. The result revealed 35% and 28% esters conversion in the reactions containing chicken viscera oil, using Novozym 435 and the crude extract respectively. The extract was also used in a reaction with soybean oil, traditionally used as starting substrate for biodiesel production.

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Pacheco, S. , Júnior, A. , Morgado, A. , Júnior, A. , Amadi, O. , Guisán, J. and Pessela, B. (2015) Isolation and Screening of Filamentous Fungi Producing Extracellular Lipase with Potential in Biodiesel Production. Advances in Enzyme Research, 3, 101-114. doi: 10.4236/aer.2015.34011.

Conflicts of Interest

The authors declare no conflicts of interest.


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