Effect of Chitosan Supplemented Diet on Survival, Growth, Feed Utilization, Body Composition & Histology of Sea Bass (Dicentrarchus labrax)

DOI: 10.4236/wjet.2015.34C005   PDF   HTML   XML   4,706 Downloads   5,951 Views   Citations


The effect of chitosan incorporated into feed formulation on the growth, feed utilization, body composition and histological response of sea bass (Dicentrarchus labrax) was investigated. Final fish weight, feed conversion ratio (FCR), specific growth rate (SGR), protein efficiency ratio (PER), protein productive value (PPV), and energy utilization (EU) of sea bass feed chitosan supplemented diet and the control diet were determined at the end of experiment. Data presents mean ± SD from triplicate determination (n = 3) for 75 days feeding trial. Different concentrations (0.5, 1.0, 2.0, 3.0 & 4.0) and control without chitosan incorporated with total fish feed in the form of dry diets were fed for 75 days. Average final weight (FW) was significantly higher at diet 3 (1 g?kg?1 chitosan) and the lowest in control fish group, specific growth rate (SGR) was significant at diet 3. Also, protein efficiency ratio (PER), protein productive value (PPV), and energy utilization (EU) showed the same trend. The chemical body composition, crude protein, dry matter, crude fat and ash were significant (P ≤ 0.01) different compared to control groups. The present investigation suggested that the chitosan incorporated into diets of sea bass fish certainly enhanced the non specific responses and reduced mortality and also improved the growth performance of fish.

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Zaki, M. , Salem, M. , Gaber, M. and Nour, A. (2015) Effect of Chitosan Supplemented Diet on Survival, Growth, Feed Utilization, Body Composition & Histology of Sea Bass (Dicentrarchus labrax). World Journal of Engineering and Technology, 3, 38-47. doi: 10.4236/wjet.2015.34C005.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] FAO (2012) The State of World Fisheries and Aquaculture Rome. 2014; p1-40.
[2] Crini, G. (2005) Recent Developments in Polysaccharide-Based Materials Used as Absorbents in Wastewater Treatment. Progress in Polymer Science, 30, 38-70. http://dx.doi.org/10.1016/j.progpolymsci.2004.11.002
[3] Huang, R.L., Yin, Y.L., Li, M.X., Wu, G.Y., Li, T.J., Li, L.L., Yang, C.B., Zhang, J., Wang, B.Z., Deng, Y., Zhang, Y.G., Tang, Z.R., Kang, P. and Guo, Y.M. (2007) Dietary Oligochitosan Supplementation Enhances Immune Status of Broilers. Journal Science of Food Agriculture, 87, 153-159. http://dx.doi.org/10.1002/jsfa.2694
[4] Li, H.Y., Yan, S.M., Shi, B.L. and Guo, X.Y. (2009) Effect of Chitosan on Nitric Oxide Content and Inducible Nitric Oxide Synthase Activity in Serum and Expression of Inducible Nitric Oxide Synthase mRNA in Small Intestine of Broiler Chickens. Asian-Aust. Journal Animal Science, 22, 1048-1053.
[5] Knaul, J.Z., Hudson, S.M. and Creber, K.A.M. (1999) Cross Linking of Chitosan Fibers with Dialdehydes: Proposal of a New Reaction Mechanism. Journal of Polymer Science Part B, Polymer Physics, 37, 1079-1094. http://dx.doi.org/10.1002/(SICI)1099-0488(19990601)37:11<1079::AID-POLB4>3.0.CO;2-O
[6] Wang, S.Q. and Zhang, C.S. (2004) Chitin, Chitosan and Their Applications in Aquaculture. Feed Res, 5, 25-28.
[7] Xu, C.L. and. Wang, Y.Z. (2005) The Applications of Chitin in Aquaculture. China Feed, 7, 30-32.
[8] Goiri, I., Oregui, L.M. and Garcia-Rodriguez, A. (2010) Use of Chitosan to Modulate Ruminal Fermentation of a 50:50 Forage-to-Concentrate Diet in Sheep. J. Anim. Sci, 88, 749-755. http://dx.doi.org/10.2527/jas.2009-2377
[9] Liao, F.H., Shieh, M.J., Chang, N.C and Chien. Y.W. (2007) Chitosan Supplementation Lowers Serum Lipids and Maintains Normal Calcium, Magnesium, and Iron Status in Hyperlipidemic Patients. Nutrition Research, 27, 146-151. http://dx.doi.org/10.1016/j.nutres.2007.01.009
[10] Pusateri, A.E., Holcomb, J.B., Kheirabadi, B.S., Alam, H.B.; Wade, C.E. and Ryan, K.L. (2006) Making Sense of the Preclinical Literature on Advanced Hemostatic Products. J. Trauma-Injury Infection and Critical Care, 60, 674-682. http://dx.doi.org/10.1097/01.ta.0000196672.47783.fd
[11] Dai, T., Tegos, G.P., Burkatovskaya, M., Castano, A.P. and. Hamblin, M.R. (2009) Chitosan Acetate Bandage as a topical Antimicrobial Dressing for Infected Burns. Antimicrob. Agents Chemother, 53, 393-400. http://dx.doi.org/10.1128/AAC.00760-08
[12] Tsukada, K., Matsumoto, T., Aizawa, K., Tokoro, A., Naruse, R., Suzuki, S. and Suzuki, M. (1990) Antimetastatic and Growth-Inhibitory Effects of N-Acetylchitohexaose in Mice Bearing Lewis Lung Carcinoma. Japanese Journal Cancer Research, 81, 259-265. http://dx.doi.org/10.1111/j.1349-7006.1990.tb02559.x
[13] Koide, S.S. (1998) Chitin-Chitosan: Properties, Benefits and Risks. Nutrition Research, 18, 1091-1101. http://dx.doi.org/10.1016/S0271-5317(98)00091-8
[14] Limam, Z., Selmi, S., Sadok, S. and El-abed, A. (2011) Extraction and Characterization of Chitin and Chitosan from Crustacean By-Products: Biological and Physicochemical Properties. African Journal Biotechnology, 10, 640-647.
[15] Benhabiles, M.S., Salah, R., Lounici, H., Drouiche, N., Goosen, M.F.A. and Mameri, N. (2012) Antibacterial Activity of Chitin, Chitosan and Its Oligomers Prepared from Shrimp Shell Waste. Food Hydrocoll, 29, 48-56. http://dx.doi.org/10.1016/j.foodhyd.2012.02.013
[16] Yao, H.T., Huang, S.Y. and Chiang, M.T. (2006) Effect of Chitosan on Plasma Cholesterol and Glucose Concentration in Streptozotocin-Induced Diabetic Rats. Taiwan. J. Agric. Chem. Food Sci, 44, 122-132.
[17] Yao, H.T., Huang, S.Y and Chiang, M.T. (2008) A Comparative Study on Hypoglycemic and Hypocholesterolemic Effects of High and Low Molecular Weight Chitosan in Streptozotocin-Induced Diabetic Rats. Food Chemistry, Toxicology, 46, 1525-1534. http://dx.doi.org/10.1016/j.fct.2007.12.012
[18] Moon, J.S., Kim, H.K., Koo, H.C., Joo, Y.S., Nam, H.M., Park, Y.H. and Kang, M.I. (2007) The Antibacterial and Immunostimulative Effect of Chitosan-Oligosaccharides against Infection by Staphylococcus aureus Isolated from Bovine Mastitis. Appl. Microbiol. Biotechnol, 75, 989-998. http://dx.doi.org/10.1007/s00253-007-0898-8
[19] Yin, Y.L., Tang, Z.R., Sun, Z.H., Liu, Z.Q., Li, T.J., Huang, R.L., Ruan, Z., Deng, Z.Y., Gao, B., Chen, L.X., Wu, G.Y. and Kim. S. (2008) Effect of Galacto-Mannan-Oligosaccharides or Chitosan Supplementation on Cytoimmunity and Humoral Immunity Response in Early-Weaned Piglets. Asian-Aust. Journal Animal Science, 21, 723-731.
[20] Huang, R.L., Yin, Y.L., Wu, G.Y., Zhang, Y.G., Li, T.J., Li, L.L., Li, M.X., Tang, Z.R., Zhang, J., Wang, B., He, J.H. and Nie, X.Z. (2005) Effect of Dietary Oligochitosan Supplementation on Ileal Digestibility of Nutrients and Performance in Broilers. Poultry Science, 84, 1383-1388. http://dx.doi.org/10.1093/ps/84.9.1383
[21] Shi, B.L., Li, D.F., Piao, X.S. and Yan, S.M. (2005) Effects of Chitosan on Growth Performance and Energy and Protein Utilization in Broiler Chickens. British Poultry Science, 46, 516-519. http://dx.doi.org/10.1080/00071660500190785
[22] Khambualai, O., Yamauchi, K., Tangtaweewipat, S. and Cheva-Isarakul, B. (2009) Growth Performance and Intestinal Histology in Broiler Chickens Fed with Dietary Chitosan. British Poultry Science, 50, 592-597. http://dx.doi.org/10.1080/00071660903247182
[23] Yuan, S.B. and Chen, H. (2012) Effects of Dietary Supplementation Of chitosan on Growth Performance and Immune Index in Ducks. African Journal of Biotechnology, 11, 3490-3495.
[24] Horwitz, W. (2006) Official Methods of Analysis. 18th Edition, AOAC (Association of Official Analytical Chemists), Washington DC, 1018.
[25] Carson, F. (1992) Histotechnology: A Self-Instructional Text. ASCP Press, 19.
[26] Holden, M.J. and Raitt, D.F.S., Eds., (1975) Manual de ciencia pesquera. parte 2. Métodos para investigar los recursos y su aplicación. Doc Tec FAO Pesca, 115, 211.
[27] Golterman, H.L., Clymo, R.S. and Ohnstad, M.A.M. (1978) Methods of Physical and Chemical Analysis of Fresh Waters. Blackwell Scientific Publications, Oxford, 214 p.
[28] Cho, C.Y. and Kaushik, S.J. (1985) Effect of Protein Intake on Metabolizable and Net Energy Values of Fish Diets. In: Cowey, C.B., Mackie, A.M. and Bell, J.G., Eds., Nutrition and Feeding in Fish, Academic Press, London, 95-117.
[29] Statistical Analysis System (1993) SAS/STAT User’s Guide Release 6.03 edn. SAS Institute Inc., Cary.
[30] Zar, J.H. (1984) Biostatistician Analysis. Prentice Hall, Englewood Cliffs.
[31] Smith, F., Clark, J E., Overman, B.L., Tozel, C.C., Huang, J.H., Rivier, J.E., Blisklager, A.T. and Moeser, A.J. (2010) Early Weaning Stress Impairs Development of Mucosal Barrier Function in the Porcine Intestine. Am. J. Physiol. Gastrointest. Liver Physiology, 298, 352-363. http://dx.doi.org/10.1152/ajpgi.00081.2009
[32] Peace, R.M., Campbell, J., Polo, J., Crenshaw, J., Russell, L. and Moeser. A.J. (2011) Spray-Dried Porcine Plasma Influences Intestinal Barrier Function, Inflammation, and Diarrhea in Weaned Pigs. J. Nutrition, 141, 1312-1317. http://dx.doi.org/10.3945/jn.110.136796
[33] Kim, J.C., Hansen, C.F., Mullana, B.P. and Pluske, J.R. (2012) Nutrition and Pathology of Weaned Pigs: Nutritional Strategies to Support Barrier Function in the Gastrointestinal Tract. Animal Feed Science Technology, 173, 3-16. http://dx.doi.org/10.1016/j.anifeedsci.2011.12.022
[34] Eugene, T.W. and Russell, J.B. (2013) Endocrine Regulation of Compensatory Growth in Fish. Front Endocrinol (Lausanne), 4, 74-87.
[35] Pell, J.M. and Bates, P.C. (1990) The Nutritional Regulation of Growth Hormone Action. Nutrition Research Review, 3, 163-192. http://dx.doi.org/10.1079/NRR19900011
[36] Canosa, L.F., Unniappan, S. and Peter, R.E. (2005) Periprandial Changes in Growth Hormone Release in Goldfish: Role of Somatostatin, Ghrelin, and Gastrin-Releasing Peptide. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 289, 125-13310. http://dx.doi.org/10.1152/ajpregu.00759.2004
[37] Baeverfjord, G. and Krogdahl, A. (1996) Development and Regression of Soybean Meal Induced Enteritis in Atlantic Salmon, Salmo salar L., Distal Intestine: A Comparison with the Intestines of Fasted Fish. Journal of Fish Diseases, 19, 375-387. http://dx.doi.org/10.1111/j.1365-2761.1996.tb00376.x
[38] Xu, Z.R., Hu, C.H., Xia, M.S., Zhan, X.A. and Wang, M.Q. (2003) Effects of Dietary Fructooligos Accharide on Digestive Enzyme Activities, Intestinal Microflora and Morphology of Male Broilers. Poultry Science, 82, 1030-1036. http://dx.doi.org/10.1093/ps/82.6.1030
[39] Hu, C.H., Gu, L.Y., Luan, Z.S., Song, J. and Zhu, K. (2012) Effects of Montmorillonite-Zinc Oxide Hybrid on Performance, Diarrhea, Intestinal Permeability and Morphology of Weanling Pigs. Animal Feed Science Technology, 177, 108-115. http://dx.doi.org/10.1016/j.anifeedsci.2012.07.028
[40] Montagne, L., Pluske, J.R. and Hampson, D.J. (2003) A Review of Interactions between Dietary Fibre and the Intestinal Mucosa, and Their Consequences on Digestive Health in Young Non-Ruminant Animals. Animal Feed Science Technology, 108, 95-117. http://dx.doi.org/10.1016/S0377-8401(03)00163-9
[41] Torzsas, T.L., Kendall, C.W., Sugano, M., Iwamoto, Y. and Rao, A.V. (1996) The Influence of High and Low Molecular Weight Chitosan on Colonic Cell Proliferation and Aberrant Crypt Foci Development in CF1 Mice. Food Chemistry and Toxicology, 34, 73-77. http://dx.doi.org/10.1016/0278-6915(95)00083-6
[42] Han, X.Y., Du, W.L., Huang, Q.C., Xu, Z.R. and Wang, Y.Z. (2012) Changes in Small Intestinal Morphology and Digestive Enzyme Activity with Oral Administration of Copper-Loaded Chitosan Nanoparticles in Rats. Biological Trace Elements Research, 145, 355-360. http://dx.doi.org/10.1007/s12011-011-9191-x
[43] Xu, Y.Q., Shi, B.L., Li, J.L., Li, T.Y., Guo, Y.W., Tian, L.X., Fu, X.Z. and Hong, L. (2012) Effects of Chitosan on Intestinal Flora in Weaned Pigs. Feed Res, 10, 54-56.
[44] Madan, M., Bhanja, S.K. and Yasmeen, B. (2009) Performance of Chitin Incorporated Diet on the Indigenous Kumaon Himalayan Fishes: Snow Trout, Schizothorax richardsonii (Gray) and Golden Mahseer, Tor putitora (Hamilton). Indian J. Fish., 56, 135-137.
[45] El-Saidy, D.M.S. and Gaber, M.M. (1998) Evaluation of the Nutritional Effects of Using Different Levels of Molasses in Prepared Practical Diets for Nile Tilapia Oreochromis niloticus L Fry. Menofiya Journal of Agriculture Research, 23, 591-602

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