Phylogenetic Study of Acacia Species Using the Molecular Marker


Acacia species are found at various arid and semiarid regions. Among the tree genera of family, Fabaceae, Acacia contains the highest number of species. We have collected different species of Acacia from various places of Saudi Arabia and reconstructed phylogeny for evaluation of genetic relationship among them. Internal transcribed spacer sequence of nrDNA (nrDNA-ITS) locus was used for the reconstruction of phylogeny among these species. Based on phylogenetic tree, Acacia etbaica and A. johnwoodii were close to each other. Similarly, A. ehrenbergiana and A. tortilis were close to each other. Thus, this gene locus was helpful in evaluating the genetic relationship among these species.

Share and Cite:

Alaklabi, A. (2015) Phylogenetic Study of Acacia Species Using the Molecular Marker. American Journal of Plant Sciences, 6, 3139-3143. doi: 10.4236/ajps.2015.619305.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Ibrahim, A.A.M. and Aref, I.M. (2000) Host Status of Thirteen Acacia Species to Meloidogyne jovanica. Journal of Nematology, 32, 609-613.
[2] Aref, I.M. (2000) Morphological Characteristics of Seeds and Seedling Growth of Some Native Acacia Trees in Saudi Arabia. Journal of King Saud University, Agricultural Sciences, 12, 31-95.
[3] Sahni, M. (1968) Important Trees of the Northern Sudan. Khartoum University Press, 40-63.
[4] Elkhalifa, K.F. (1996) Forest Botany. Khartoum University Press, 79-94.
[5] Williames, J.K., Kubelik, A., Livak, K., Rafalsky, J. and Tingey, S. (1990) DNA Polymorphisms Amplified by Arbitrary Primer and Useful as Genetic Markers. Nucleic Acids Research, 18, 6531-6535.
[6] Vos, R., Hogers, R., Bleeker, M., Reijans, M., Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J. and Kuiper, M. (1995) AFLP: A New Technique for DNA Fingerprinting. Nucleic Acids Research, 23, 4407-4414.
[7] Beckman, J.S. and Soller, M. (1983) Restriction Fragment Length Polymorphisms in Genetic Improvement: Methodologies, Mapping and Costs. Theoretical and Applied Genetics, 67, 35-43.
[8] Butcher, P.A., Moran, G.F. and Perkins, H.D. (1998) RFLP Diversity in the Nuclear Genome of Acacia mangium. Heredit, 81, 205-213.
[9] McGranahan, M., Bell, J.C., Moran, G.F. and Slee, M. (1997) High Genetic Divergence between Geographic Regions in the Highly out Crossing Species Acacia aulacocarpa (Cunn. Ex Benth.). Forest Genetics, 4, 1-13.
[10] Playford, J., Bell, J.C. and Moran, G.F. (1993) A Major Disjunction in Genetic Diversity over the Geographic Range of Acacia Melanoxylon R. Australian Journal of Botany, 41, 355-368.
[11] Ashworth, S. (2002) Seed to Seed. 2nd Edition. Seed Savers Exchange, Decorah.
[12] Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739.
[13] Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997) The Clustal X Windows Interface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools. Nucleic Acids Research, 24, 4876-4882.
[14] Nabli, M.A. (1989) Essai de Synthèse sur la Végétation et la Phyto-écologie Tunisienne. Faculté des Sciences, Tunis.
[15] Nanda, R.M., Nayak, S. and Rout, G.R. (2004) Studies on Genetic Relatedness of Acacia Tree Species Using RAPD Markers. Biologia, Bratislava, 59, 115-120.
[16] Widyatmoko, A.Y.P.B.C., Watanabe, A. and Shiraishi, S. (2010) Study on Genetic Variation and Relationships among Four Acacia Species Using RAPD and SSCP Marker. Journal of Forestry Research, 7, 125-143.
[17] Josiah, C.C., George, D.O., Eleazar, O.M. and Nyamu, W.F. (2008) Genetic Diversity in Kenyan Populations of Acacia senegal (L.) Willd revealed by Combined RAPD and ISSR Markers. African Journal of Biotechnology, 7, 2333-2340.
[18] Sirelkhatem, R. and Gaali, E.E.L. (2009) Phylogenic Analysis in Acacia senegal Using AFLP Molecular Markers across the Gum Arabic Belt in Sudan. African Journal of Biotechnology, 8, 4817-4823.
[19] Li, J.-H., Liu, Z.-J., Salazar, G.A., Bernhardt, P., Perner, H., Tomohisa, Y., Jin, X.-H., Chung, S.-W. and Luo, Y.-B. (2011) Molecular Phylogeny of Cypripedium (Orchidaceae: Cypripedioideae) Inferred from Multiple Nuclear and Chloroplast Regions. Molecular Phylogenetics and Evolution, 61, 308-320.
[20] Harpke, D., Meng, S., Rutten, T., Kerndorff, H. and Blattner, F.R. (2012) Phylogeny of Crocus (Iridaceae) Based on One Chloroplast and Two Nuclear Loci: Ancient Hybridization and Chromosome Number Evolution. Molecular Phylogenetics and Evolution, 66, 617-627.
[21] Zhao, G.J., Yanga, Z.Q., Chen, X.P. and Guo, Y.H. (2011) Genetic Relationships among Loquat Cultivars and Some Wild Species of the Genus Eriobotrya Based on the Internal Transcribed Spacer (ITS) Sequences. Scientia Horticulturae, 130, 913-918.

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.