Significance of Golgi Protein 73, Alpha-Fetoprotein and Vascular Endothelial Growth Factor Expression in Diagnosis of Primary Hepatic Cancer

Abstract Full-Text HTML XML Download Download as PDF (Size:336KB) PP. 1145-1150
DOI: 10.4236/jct.2015.613124    1,932 Downloads   2,248 Views   Citations

ABSTRACT

Aim: We measured Golgi protein73 (GP73), alpha-fetoprotein (AFP), and vascular endothelial growth factor (VEGF) expression in primary hepatic cancer (PHC) and assessed their clinical significance. Methods: Forty-five PHC and tumor-adjacent specimens and 14 normal liver specimens were examined. GP73, AFP, and VEGF expression was measured via immunohistochemistry and a correlation of protein expression with clinical pathology of PHC was suggested. Results: GP73, AFP, and VEGF expression was significantly higher in PHC and tumor-adjacent tissue compared to tumor-adjacent tissue (0.143 ± 0.018 vs. 0.124 ± 0.027, 0.116 ± 0.026 vs. 0.098 ± 0.014, and 0.126 ± 0.027 vs. 0.092 ± 0.016, respectively; all p < 0.05) and normal liver tissue (0.124 ± 0.027 vs. 0.088 ± 0.029, 0.098 ± 0.014 vs. 0.073 ± 0.011, and 0.092 ± 0.016 vs. 0.076 ± 0.018, respectively; all p < 0.05), respectively. GP73 expression was positively correlated with pathological grade and cirrhosis, but not with tumor size, nodules, clinical stage and serum AFP. VEGF expression was positively correlated with tumor size, nodules, portal vein tumor thrombus, and clinical stage, but not with the degree of tumor differentiation and serum AFP. Expression of GP73 and VEGF was greater than that of AFP in PHC (both p < 0.05). Conclusion: GP73 is highly expressed in PHC and may be a diagnostic marker. Combined detection of GP73, AFP, and VEGF is helpful for diagnosis of PHC.

Cite this paper

Li, X. , Li, X. , Zhou, W. , Wang, G. and Wang, W. (2015) Significance of Golgi Protein 73, Alpha-Fetoprotein and Vascular Endothelial Growth Factor Expression in Diagnosis of Primary Hepatic Cancer. Journal of Cancer Therapy, 6, 1145-1150. doi: 10.4236/jct.2015.613124.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Marrero, J.A., Romano, P.R., Nikolaeva, O., Steel, L., Mehta, A., Fimmel, C.J., Comunale, M.A., D’Amelio, A., Lok, A.S. and Block, T.M. (2005) GP73, a Resident Golgi Glycoprotein, Is a Novel Serum Marker for Hepatocellular Carcinoma. Journal of Hepatology, 43, 1007-1012.
http://dx.doi.org/10.1016/j.jhep.2005.05.028
[2] Shen, H. (1995) Quantitative Methods of Immunohistochemical Staining (III). Chinese Journal of Histochemistry and Cytochemistry, 4, 89-91.
[3] Block, T.M., Comunale, M.A., Lowman, M., Steel, L.F., Romano, P.R., Fimmel, C., Tennant, B.C., London, W.T., Evans, A.A, Blumberg, B.S., Dwek, R.A., Mattu, T.S. and Mehta, A.S. (2005) Use of Targeted Glycoproteomics to Identify Serum Glycoproteins that Correlate with Liver Cancer in Woodchucks and Humans. Proceedings of the National Academy of Sciences of the United States of America, 102, 779-784.
http://dx.doi.org/10.1073/pnas.0408928102
[4] Kladney, R.D., Cui, X., Bulla, G.A, Brunt, E.M. and Fimmel, C.J. (2002) Expression of GP73, a Resident Golgi Membrane Protein, in Viral and Nonviral Liver Disease. Hepatology, 35, 1431-1440.
http://dx.doi.org/10.1053/jhep.2002.32525
[5] Wei, S., Dunn, T.A., Isaacs, W.B., De Marzo, A.M. and Luo, J. (2008) GOLPH2 and MYO6: Putative Prostate Cancer Markers Localized to the Golgi Apparatus. Prostate, 68, 1387-1395.
http://dx.doi.org/10.1002/pros.20806
[6] Iftikhar, R., Kladney, R.D., Havlioglu, N., Schmitt Graff, A., Gusmirovic, I., Solomon, H., Luxon, B.A., Bacon, B.R. and Fimmel, C.J. (2004) Disease and Cell-Specific Expression of GP73 in Human Liver Disease. The American Journal of Gastroenterology, 99, 1087-1095.
http://dx.doi.org/10.1111/j.1572-0241.2004.30572.x
[7] Poon, R.T., Lau, C., Pang, R., Ng, K.K., Yuen, J. and Fan, S.T. () High Serum Vascular Endothelial Growth Factor Levels Predict Poor Prognosis after Radiofrequency Ablation of Hepatocellular Carcinoma: Importance of Tumor Biomarker in Ablative Therapies. Annals of Surgical Oncology, 14, 1835-1845.
http://dx.doi.org/10.1245/s10434-007-9366-z
[8] Kawarai, S., Hashizaki, K., Kitao, S., Nagano, S., Madarame, H., Neo, S., Ishikawa, T., Furuichi, M., Hisasue, M., Tsuchiya, R., Tsujimoto, H. and Yamada, T. (2006) Establishment and Characterization of Primary Canine Hepatocellular Carcinoma Cell Lines Producing Alpha-Fetoprotein. Veterinary Immunology and Immunopathology, 113, 30-36.
http://dx.doi.org/10.1016/j.vetimm.2006.03.006

  
comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.