A New Tool for Resistance Management: Baseline Toxicity, Ovicidal Activity, and Field Efficacy of the Novel Insecticide Tolfenpyrad on Colorado Potato Beetle, Leptinotarsa decemlineata

Adam F. Wimer^1*, Christopher R. Philips², Thomas P. Kuhar¹, James C. Adams³, Zsofia Szendrei⁴

¹Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, USA.
²Department of Entomology, North Central Research & Outreach Center, University of Minnesota, Grand Rapids, USA.
³Nichino America, Inc., Newark, USA.
⁴Department of Entomology, Michigan State University, East Lansing, USA.

DOI: 10.4236/ae.2015.34017                

Abstract

Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) is one of the most important insect pests of potato (Solanum tuberosum L.). Because of the high value of potato crops, most growers approach management in a similar manner. Chemical control of arthropod pests in potato is the standard pest management practice, and will likely continue to be in the foreseeable future. This heavy reliance on chemical control has led to high levels of insecticide resistance. Strategies that rotate chemistries are critical in order to maintain insecticide efficacy, highlighting the immediate need to evaluate novel chemistries to continue to manage this pest successfully. Working with different populations of L. decemlineata, field and lab experiments were conducted to evaluate the baseline toxicity, ovicidal activity, and field efficacy of the novel insecticide tolfenpyrad to L. decemlineata. Lab assays revealed that tolfenpyrad was toxic to both larvae and adults, and that L. decemlineata treated egg masses had a 0% hatch rate. Potato field plots treated with tolfenpyrad had significantly fewer larvae, less defoliation, and higher tuber yields. These data will provide accurate field rates for proper labeling, a baseline reference for tracking changes in L. decemlineata susceptibility, as well as provide a novel chemistry to aid in resistance management programs.

Keywords

Toxicity, Tolfenpyrad, Colorado Potato Beetle, Leptinotarsa decemlineata, LC50, Resistance Management

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Capinera, J.L. (2001) Handbook of Vegetable Pests. Academic Press, New York.
[2]	Alyokhin, A. (2009) Colorado Potato Beetle Management on Potatoes: Current Challenges and Future Prospects. Fruit, Vegetable and Cereal Science and Biotechnology, 1, 10-19.
[3]	Hare, J.D. (1990) Ecology and Management of the Colorado Potato Beetle. Annual Review of Entomology, 35, 81-100. http://dx.doi.org/10.1146/annurev.en.35.010190.000501
[4]	Hare, J.D. (1980) Impact of Defoliation by the Colorado Potato Beetle on Potato Yields. Journal of Economic Entomology, 73, 369-373. http://dx.doi.org/10.1093/jee/73.3.369
[5]	Kuhar, T.P., Mori, K. and Dickens, J.C. (2006) Potential of a Synthetic Aggregation Pheromone for Integrated Pest Management of Colorado Potato Beetle. Agricultural and Forest Entomology, 8, 77-81. http://dx.doi.org/10.1111/j.1461-9555.2006.00286.x
[6]	Kuhar, T.P., Doughty, H., Hitchner, E. and Cassell, M.E. (2008) Evaluation of Foliar Insecticides for the Control of Colorado Potato Beetle in Potatoes in Virginia, 2007. Arthropod Management Tests, 33, E8. http://dx.doi.org/10.1093/amt/33.1.E8
[7]	Kuhar, T.P. and Doughty, H. (2009) Evaluation of Foliar Insecticides for the Control of Colorado Potato Beetle in Potatoes, 2008. Arthropod Management Tests, 34, E71.
[8]	Ferro, D.N. (1993) Potential for Resistance to Bacillus thuringiensis: Colorado Potato Beetle (Coleoptera: Chrysomelidae) a Model System. American Entomologist, 39, 38-44. http://dx.doi.org/10.1093/ae/39.1.38
[9]	Bishop, B.A. and Grafius, E.J. (1996) Insecticide Resistance in the Colorado Potato Beetle. In: Jolivet, P. and Hsiao, T.H., Eds., Chrysomelidae Biology, SBP Academic Publishing, Amsterdam.
[10]	Alyokhin, A.V., Baker, M., Mota-Sanchez, D., Dively, G. and Grafius, E. (2008) Colorado Potato Beetle Resistance to Insecticides. American Journal of Potato Research, 85, 395-413. http://dx.doi.org/10.1007/s12230-008-9052-0
[11]	Whalon, M.E. and Ferro, D.N. (1998) Bt-Potato Resistance Management. In: Mellon, M. and Rissler, J., Eds., Now or Never: Serious New Plans to Save a Natural Pest Control, UCS, Cambridge, 107-136.
[12]	Huseth, A.S., Groves, R.L., Chapman, S.A., Alyokhin, A., Kuhar, T.P., Macrae, I.V., Szendrei, Z. and Nault, B.A. (2014) Managing Colorado Potato Beetle Insecticide Resistance: New Tools and Strategies for the Next Decade of Pest Control in Potato. Journal of Integrated Pest Management, 5, 1-8. http://dx.doi.org/10.1603/IPM14009
[13]	Whalon M., Mota-Sanchez, D., Hollingworth, R. and Duynslager, L. (2015) Arthropod Pesticide Resistance Database. http://www.pesticideresistance.com/
[14]	Grafius, E. (1997) Economic Impact of Insecticide Resistance in the Colorado Potato Beetle (Coleoptera: Chrysomelidae) on the Michigan Potato Industry. Journal of Economic Entomology, 90, 1144-1151. http://dx.doi.org/10.1093/jee/90.5.1144
[15]	Huseth, A.S. and Groves, R.L. (2013) Effect of Insecticide Management History on Emergence Phenology and Neonicotinoid Resistance in Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 106, 2491-2505. http://dx.doi.org/10.1603/EC13277
[16]	Chen, J., Alyokhin, A., Mota-Sanchez, D., Baker, M. and Whalon, M. (2014) Variation in Fitness among Geographically Isolated Colorado Potato Beetle (Coleoptera: Chrysomelidae) Populations. Annals of Entomological Society of America, 107, 128-135. http://dx.doi.org/10.1603/AN13018
[17]	Kuhar, T.P., Kamminga, K., Philips, C., Wallingford, A. and Wimer, A. (2013) Chemical Control of Potato Pests. In: Giordanengo, P., Vincent, C. and Alyokhin, A., Eds., Insect Pests of Potato: Global Perspectives on Biology and Management, Elsevier, Waltham, MA, 375-397. http://dx.doi.org/10.1016/B978-0-12-386895-4.00013-2
[18]	Mota-Sanchez, D., Hollingworth, R.M., Grafius, E.J. and Moyer, D.D. (2006) Resistance and Cross-Resistance to Neonicotinoid Insecticides and Spinosad in the Colorado Potato Beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). Pest Management Science, 62, 30-37. http://dx.doi.org/10.1002/ps.1120
[19]	Szendrei, Z., Grafius, E., Byrne, A. and Ziegler, A. (2012) Resistance to Neonicotinoid Insecticides in Field Populations of the Colorado Potato Beetle (Coleoptera: Chrysomelidae). Pest Management Science, 68, 941-946. http://dx.doi.org/10.1002/ps.3258
[20]	Nonaka, N. (2003) Tolfenpyrad—A New Insecticide with Wide Spectrum and Unique Action. Agrochemicals Japan, 83, 17-19.
[21]	Anonymous (2012) Tofenpyrad Insecticide: A New Broad-Spectrum Insecticide from Nichino America, Inc. Technical Bulletin, TOL-TIB-1201.
[22]	USDA (2011) Agricultural Marketing Service. United States Standards for Grades of Potatoes.
[23]	Motulsky, H.J. (2007) Prism 5 Statistics Guide. Graph Pad Software Inc., San Diego.
[24]	Abbott, W.S. (1925) A Method of Computing the Effectiveness of an Insecticide. Journal of Economic Entomology, 18, 265-267. http://dx.doi.org/10.1093/jee/18.2.265a
[25]	SAS (2013) JMP Version 10. SAS Institute, Cary.
[26]	Natwick, E.T. (2012) Insecticide Efficacy against Thrips in Romaine Lettuce, Spring 2011. Arthropod Management Tests, 37, E26.
[27]	Kuhar, T.P., Schultz, P., Doughty, H., Wimer, A., Wallingford, A., Andrews, H., Phillips, C., Cassell, M. and Jenrette, J. (2011) Evaluation of Foliar Insecticides for the Control of Green Peach Aphid in Broccoli in Virginia, 2010. Arthropod Management Tests, 36, E4.
[28]	Kuhar, T.P., Schultz, P., Doughty, H., Wimer, A., Wallingford, A., Andrews, H., Phillips, C., Cassell, M. and Jenrette, J. (2011) Evaluation of Foliar Insecticides for the Control of Insects in Potatoes in Virginia, 2010. Arthropod Management Tests, 36, E64.
[29]	Burrack, H. and Chapman, A. (2012) Tomato Spotted Wilt Virus Management in Tobacco with Unregistered Materials, 2011. Arthropod Management Tests, 37, F90.
[30]	Trisyono, A. and Whalon, M.E. (1999) Toxicity of Neem Applied Alone and in Combinations with Bacillus thuringiensis to Colorado Potato Beetle (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 92, 1281-1288. http://dx.doi.org/10.1093/jee/92.6.1281
[31]	Kowalska, J. (2007) Azadirachtin as a Product for Control of Colorado Potato Beetles. Journal of Research and Applications in Agricultural Engineering, 52, 78-81.
[32]	Ghidiu, G. and Zehnder, G.W. (1993) Timing of the Initial Spray Application of Bacillus thuringiensis for Control of the Colorado Potato Beetle (Coleoptera: Chrysomelidae) in Potatoes. Biological Control, 3, 348-352. http://dx.doi.org/10.1006/bcon.1993.1046
[33]	Sirota, J.M. and Grafius, E. (1994) Effects of Cryomazine on Larval Survival, Pupation, and Adult Emergence of Colorado Potato Beetle (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 87, 577-582. http://dx.doi.org/10.1093/jee/87.3.577
[34]	Linduska, J.J., Ross, M., Mulford, K. and Baumann, D. (1996) Colorado Potato Beetle Control with Foliar Insecticide Sprays, 1995. Arthropod Management Tests, 21, E28.
[35]	Byrne, A.M. and Szendrei, Z. (2012) Colorado Potato Beetle Control, 2011. Arthropod Management Tests, 37, E46
[36]	Byrne, A.M. and Szendrei, Z. (2013) Colorado Potato Beetle Control, 2012. Arthropod Management Tests, 38, E49.
[37]	Alyokhin, A., Dively, G., Patterson, M., Mahoney, M., Castaldo, C., Rogers, D. and Wollam, J. (2007) Resistance and Cross-Resistance to Imidacloprid and Thiamethoxam in the Colorado Potato Beetle Leptinotarsa decemlineata. Pest Management Science, 63, 32-41. http://dx.doi.org/10.1002/ps.1305
[38]	Alyokhin, A., Dively, G., Patterson, M., Mahoney, M., Castaldo, C., Rogers, D. and Wollam, J. (2007) Susceptibility of Imidacloprid-Resistant Colorado Potato Beetle to Non-Neonicotinoid Insecticides in the Laboratory and Field Trials. American Journal of Potato Research, 83, 485-494. http://dx.doi.org/10.1007/BF02883509
[39]	Roush, R.T., Hoy, C.W., Ferro, D.N. and Tingey, W.M. (1990) Insecticide Resistance in the Colorado Potato Beetle (Coleoptera: Chrysomelidae): Influence of Crop Rotation and Insecticide Use. Journal of Economic Entomology, 83, 315-319. http://dx.doi.org/10.1093/jee/83.2.315
[40]	Heim, D.C., Kennedy, G.G., Gould, F.L. and Van Duyn, J.W. (1992) Inheritance of Fenvalerate and Carbofuran Resistance in Colorado Potato Beetles, Leptinotarsa decemlineata (Say), from North Carolina. Pesticide Science, 34, 303- 311. http://dx.doi.org/10.1002/ps.2780340402
[41]	Miyo, T., Keil, C.B.O., Hough-Goldstein, J.A. and Oguma, Y. (1999) Inheritance of Resistance to Esfenvalerate in Colorado Potato Beetle (Coleoptera: Chrysomelidae) Populations. Journal of Economic Entomology, 93, 447-458.
[42]	Hawthorne, D.J. (2003) Quantitative Trait Locus Mapping of Pyrethroid Resistance in Colorado Potato Beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 96, 1021-1030. http://dx.doi.org/10.1093/jee/96.4.1021