Hysteresis of White Adipose Tissue


Objective: This study was performed to analyze the modifications within adipose tissue during calorie restriction and more specifically to state whether hysteresis occurs during fat mass reduction. Method: Rats male Wistar increased their body weight by 130 g under control conditions and were then submitted to a calorie restriction (CR) at 30% or 60% of control. Experiment has been stopped when the body weight of the group CR60% returned back to its initial value. Samples of retroperitoneal adipose tissue were collected by biopsies along the study. Adipose cell size was analyzed using multisizer IV (Beckman Coulter) to determine the size distribution curves during natural growth and after calorie restriction. Results: After CR60%, body weights and adipose tissue masses were similar to the ones at the beginning of the experiment. Adipose cell size distribution curve was shifted to the left compared to the one of initial control. Adipose cell sizes were significantly lower after CR60% than those of control at the beginning of the experiment. Conclusions: These results state for the first time that hysteresis occurs in white adipose tissue after calorie restriction. The composition of adipose tissue after calorie restriction was significantly different than the one of initial control. After significant weight loss, organisms must be considered as different from the initial controls, they are most likely governed by different regulations which will have to be identified.

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Géloën, A. , Soula, H. , Hadji, L. and Berger, E. (2015) Hysteresis of White Adipose Tissue. Open Journal of Endocrine and Metabolic Diseases, 5, 138-147. doi: 10.4236/ojemd.2015.510018.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Fasshauer, M. and Blüher, M. (2015) Adipokines in Health and Disease. Trends in Pharmacological Sciences, 36, 461-470. http://dx.doi.org/10.1016/j.tips.2015.04.014
[2] Zinder, O. and Shapiro, B. (1975) Effect of Cell Size on Epinephrine- and Acth-Induced Fatty Acid Release from Isolated Fat Cells. Journal of Lipid Research, 12, 91-95.
[3] Smith, U. (1971) Effect of Cell Size on Lipid Synthesis by Human Adipose Tissue in Vitro. Journal of Lipid Research, 12, 65-70.
[4] Gliemann, J. and Vinten, J. (1974) Lipogenesis and Insulin Sensitivity of Single Fat Cells. Journal of Physiology (London), 236, 499-516. http://dx.doi.org/10.1113/jphysiol.1974.sp010449
[5] Lafontan, M. and Berlan, M. (1995) Fat Cell Alpha 2-Adrenoceptors: The Regulation of Fat Cell Function and Lipolysis. Endocrine Reviews, 16, 716-738. http://dx.doi.org/10.1210/er.16.6.716
[6] Lay, S.L., Krief, S., Farnier, C., Lefrêre, I., Liepvre, X.L., Bazin, R., et al. (2001) Cholesterol, a Cell Size-Dependent Signal That Regulates Glucose Metabolism and Gene Expression in Adipocytes. Journal of Biological Chemistry, 276, 16904-16910. http://dx.doi.org/10.1074/jbc.M010955200
[7] Skurk, T., Alberti-Huber, C., Herder, C. and Hauner, H. (2007) Relationship between Adipocyte Size and Adipokine Expression and Secretion. Journal of Clinical Endocrinology & Metabolism, 92, 1023-1033. http://dx.doi.org/10.1210/jc.2006-1055
[8] Wueest, S., Rapold, R.A., Rytka, J.M., Schoenle, E.J. and Konrad, D. (2009) Basal Lipolysis, Not the Degree of Insulin Resistance, Differentiates Large from Small Isolated Adipocytes in High-Fat Fed Mice. Diabetologia, 52, 541-546. http://dx.doi.org/10.1007/s00125-008-1223-5
[9] Miller, W.H., Faust, I.M., Goldberger, A.C. and Hirsch, J. (1983) Effects of Severe Long-Term Food Deprivation and Refeeding on Adipose Tissue Cells in the Rat. American Journal of Physiology, 245, E74-E80.
[10] Gélo?n, A., Roy, P.E. and Bukowiecki, L.J. (1989) Regression of White Adipose Tissue in Diabetic Rats. American Journal of Physiology, 257, E547-E553.
[11] Di Girolamo, M., Fine, J.B., Tagra, K. and Rossmanith, R. (1998) Qualitative Regional Differences in Adipose Tissue Growth and Cellularity in Male Wistar Rats Fed Ad Libitum. American Journal of Physiology, 274, R1460-R1467.
[12] Hadji, L., Berger, E., Soula, H., Vidal, H. and Géloen, A. (2014) White Adipose Tissue Resilience to Insulin Deprivation and Replacement. PLoS ONE, 9, e106214. http://dx.doi.org/10.1371/journal.pone.0106214
[13] Soula, H.A., Julienne, H., Soulage, C.O. and Géloen, A. (2013) Modelling Adipocytes Size Distribution. Journal of Theoretical Biology, 332, 89-95. http://dx.doi.org/10.1016/j.jtbi.2013.04.025
[14] Soula, H.A., Gélo?n, A. and Soulage, C.O. (2015) Model of Adipose Tissue Cellularity Dynamics during Food Restriction. Journal of Theoretical Biology, 364, 189-196.
[15] Bailey, J.W., Barker, R.L. and Beauchene, R.E. (1993) Age-Related Changes in Rat Adipose Tissue Cellularity Are Altered by Dietary Restriction and Exercise. Journal of Nutrition, 123, 52-58.
[16] Ghorbani, A., Varedi, M., Hadjzadeh, M.A. and Omrani, G.H. (2010) Type-1 Diabetes Induces Depot-Specific Alterations in Adipocyte Diameter and Mass of Adipose Tissues in the Rat. Experimental and Clinical Endocrinology & Diabetes, 118, 442-448. http://dx.doi.org/10.1055/s-0030-1247566
[17] Kirchner, H., Hofmann, S.M., Fischer-Rosinsky, A., Hembree, J., Abplanalp, W., Ottaway, N., et al. (2012) Caloric Restriction Chronically Impairs Metabolic Programming in Mice. Diabetes, 61, 2734-2742.
[18] Furukawa, S.H., Fujita, T., Shimabukuro, M., Iwaki, M., Yamada, Y., Nakajima, Y., et al. (2004) Increased Oxidative Stress in Obesity and Its Impact on Metabolic Syndrome. The Journal of Clinical Investigation, 114, 1752-1761. http://dx.doi.org/10.1172/JCI21625
[19] Ramos, L.F., Shintani, A., Ikizler, T.A. and Himmelfarb, J. (2008) Oxidative Stress and Inflammation Are Associated with Adiposity in Moderate to Severe CKD. Journal of the American Society of Nephrology, 19, 593-599. http://dx.doi.org/10.1681/ASN.2007030355
[20] Park, S., Park, N.Y., Valacchi, G. and Lim, Y. (2012) Calorie Restriction with a High-Fat Diet Effectively Attenuated Inflammatory Response and Oxidative Stress-Related Markers in Obese Tissues of the High Diet Fed Rats. Mediators of Inflammation, 2012, Article ID: 984643.
[21] Barzilai, N. and Gabriely, I. (2001) The Role of Fat Depletion in the Biological Benefits of Caloric Restriction. The Journal of Nutrition, 131, 903S-906S.
[22] Weyer, C., Foley, J.E., Bogardus, C., Tataranni, P.A. and Pratley, R.E. (2000) Enlarged Subcutaneous Abdominal Adipocyte Size, but Not Obesity Itself, Predicts Type II Diabetes Independent of Insulin Resistance. Diabetologia, 43, 1498-1506. http://dx.doi.org/10.1007/s001250051560
[23] Lonn, M., Mehlig, K., Bengtsson, C. and Lissner, L. (2010) Adipocyte Size Predicts Incidence of Type 2 Diabetes in Women. The FASEB Journal, 24, 326-331. http://dx.doi.org/10.1096/fj.09-133058
[24] Andersson, D.P., Eriksson Hogling, D., Thorell, A., Toft, E., Qvisth, V., Naslund, E., et al. (2014) Changes in Subcutaneous Fat Cell Volume and Insulin Sensitivity after Weight Loss. Diabetes Care, 37, 1831-1836. http://dx.doi.org/10.2337/dc13-2395
[25] Clément, K., Viguerie, N., Poitou, C., Carette, C., Pelloux, V., Curat, C.A., et al. (2004) Weight Loss Regulates Inflammation-Related Genes in White Adipose Tissue of Obese Subjects. The FASEB Journal, 18, 1657-1669. http://dx.doi.org/10.1096/fj.04-2204com

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