Online Effective Identification of Glycopeptide Using Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS)*

Ling Xin, Li Hou, Yue Zhou, Huiping Wang, Heming Yu

National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China.

DOI: 10.4236/abb.2015.69065                

Abstract

The detailed glycan structural analysis of glycoprotein is amenable to glycopeptide enrichment. Here, we develop a simple, effective and economical approach to enrich glycopeptides from proteolytically digested peptide mixtures by chromatographic column packed with graphite carbon and activated charcoal (G/A-column). Glycopeptide from ovalbumin was efficiently enriched by homemade G/A-column using liquid chromatography and the structure of glycopeptide was obtained by tandem mass spectrometry using Fourier transform ion cyclotron resonance mass spectrometry. The results in this study demonstrate that G/A-column can be used to enrich N-glycolpeptides and be benefit for online identification of glycopeptide using LC-MS.

Keywords

Glycopeptide, LC-ESI, FTICR-MS

Conflicts of Interest

The authors declare no conflicts of interest.

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