Effects of Melatonin on Ischemia-Reperfusion Injury in Rat Ovary: Histopathologic and Immunohistochemical Study
Deniz Balsak1, Cihan Toğrul2, Cenap Ekinci3, Kerem Doğa Seçkin4, Mehmet Fatih Karsli5, Aysun Ekinci6, Ali Emre Tahaoğlu7, Hanefi Bademkiran8, Zuat Acar9, Senem Yaman Tunç10, Engin Deveci3*, Günsen Kirman3
1Department of Obstetrics and Gynecology, School of Medicine Hospital, University of Halic, Istanbul, Turkey.
2Department of Obstetrics and Gynecology, School of Medicine, University of Hitit, Corum, Turkey.
3Department of Histology and Embryology, School of Medicine Hospital, University of Dicle, Diyarbakir, Turkey.
4Department of Obstetrics and Gynecology, Kanuni Sultan Süleyman, Education and Research Hospital, Istanbul, Turkey.
5Department of Obstetrics and Gynecology, Dr. Sami Ulus, Education and Research Hospital, Ankara, Turkey.
6Department of Biochemistry, Faculty of Medicine, University of Dicle, Diyarbakir, Turkey.
7Department of Obstetrics and Gynecology, Diyarbakir Gazi Yasargil Education and Research Hospital, Diyarbakir, Turkey.
8Department of Obstetrics and Gynecology, Diyarbakir Obstetrics and Gynecology Hospital, Diyarbakir, Turkey.
9Department of Obstetrics and Gynecology, Memory Hospital, Diyarbakir, Turkey.
10Department of Obstetrics and Gynecology, School of Medicine Hospital, University of Dicle, Diyarbakir, Turkey.
DOI: 10.4236/ojog.2015.511090   PDF   HTML   XML   4,463 Downloads   5,193 Views   Citations


Aim: To investigate the effect of melatonin on ischemia–reperfusion (I/R) injury in rat ovary. Material and Method: A total of 24 Wistar rats were divided into four groups. The rats were first numbered randomly and then randomly divided into four equal groups: sham, torsion, detorsion and melatonin groups. In group I (n = 6) sham, as well as in group II ovary torsion was not performed and no drug was administered. In group III, 1 hour of ischemia and 2 hours of reperfusion were performed and no drug was given. In group IV, melatonin was dissolved in 1% ethanol just before use. Melatonin was injected 1 hour before torsion to the torsion plus melatonin group. Right ovaries were surgically removed in all groups. Result: Malondialdehyde (MDA) levels were measured. We suggested that administration of melatonin would be in treating ovaries from torsion/detorsion induced damage in humans. The histopathological changes such as vascular congestion, edema, hemorrhage, and follicular degeneration were found to be decreased in the melatonin + ischemia-reperfusion group. Ischemia-reperfusion group: PECAM-I expression is positive in vessel of the stromal area. The follicles and interfolicular area infiltrated with CD68 positive cells were increased in a time of ischemia-reperfusion exposure dependent manner. Conclusions: Melatonin after ischemia-reperfusion CD31 and CD68 expressions is weak, due to the reduction of the inflammatory effects and hemorrhage is thought that melatonin is effective.

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Balsak, D. , Toğrul, C. , Ekinci, C. , Seçkin, K. , Karsli, M. , Ekinci, A. , Tahaoğlu, A. , Bademkiran, H. , Acar, Z. , Tunç, S. , Deveci, E. and Kirman, G. (2015) Effects of Melatonin on Ischemia-Reperfusion Injury in Rat Ovary: Histopathologic and Immunohistochemical Study. Open Journal of Obstetrics and Gynecology, 5, 639-645. doi: 10.4236/ojog.2015.511090.

Conflicts of Interest

The authors declare no conflicts of interest.


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