DNA Extraction from Insects by Using Different Techniques: A Review


This review paper was prepared at Department of Zoology, University of Gujrat, Pakistan during 2014-2015. The data for the two decades, DNA Extraction from Insects by Using Different Techniques was compiled through a thorough review of many research articles published in various journals of international repute. Insects are very important economically and ecologically for human due to their various roles, as pests, decomposer in nutrition cycle, vector for the transmission of certain diseases and a source of medically and economically important products. To identify insect, and for research on their role, different molecular techniques are used. For each molecular technique, high quality DNA is required, which is extracted from the insects by different techniques. The selection of DNA extraction technique is depended upon specimen under study, time required for extraction, economical stander of technique due to reagents and equipment used for extraction and most importantly extracted DNA quality. In this paper, most frequently used techniques for DNA extraction from insect with different size and condition are outlined. This paper is only a guide for these techniques and we describe them briefly.

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Asghar, U. , Malik, M. , Anwar, F. , Javed, A. and Raza, A. (2015) DNA Extraction from Insects by Using Different Techniques: A Review. Advances in Entomology, 3, 132-138. doi: 10.4236/ae.2015.34016.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Ball, S.L. and Armstrong, K.F. (2008) Rapid, One-Step DNA Extraction for Insect Pest Identification by Using DNA Barcodes. Journal of Economic Entomology, 101, 523-532.
[2] Nancy, C.-C., Mauricio, Q., Horacio, C.-C. and Guadalupe, Z.-P. (2010) A Simple and Rapid Method for DNA Isolation from Xylophagous Insects. International Journal of Molecular Sciences, 11, 5056-5064.
[3] David, P., Caroline, W., Graeme, F., Sandra, L.K., Richard, F.P. and Terence, A.B. (2013) Absence of Ancient DNA in Sub-Fossil Insect Inclusions Preserved in “Anthropocene” Colombian Copal. PLoS One, 8, e73150.
[4] Behura, S.K. (2006) Molecular Marker Systems in Insects: Current Trends and Future Avenues. Molecular Ecology, 15, 3087-3113. http://dx.doi.org/10.1111/j.1365-294X.2006.03014.x
[5] Freitas, M.T., Gomes-Júnior, P.P., Batista, M.V., Leal-Balbino, T.C., Araujo, A.L. and Balbino, V.Q. (2014) Novel DNA Extraction Assay for Molecular Identification of Aedes spp. Eggs. Genetics and Molecular Research, 13, 8776- 8782. http://dx.doi.org/10.4238/2014.October.27.19
[6] Paul, C. (2010) Bugs and Drugs Part 1: New Alternative Medicine for 21st Century. Alternative Medicine Review, 15, 124-135.
[7] Musapa, M., Kumwenda, T., Mkulama, M., Chishimba, S., Norris, D.E., Thuma, P.E. and Mharakurwa, S. (2013) A Simple Chelex Protocol for DNA Extraction from Anopheles spp. Journal of Visualized Experiments, 71, e3281.
[8] Gariepy, T.D., Kuhlmann, U., Gillott, C. and Erlandson, M. (2007) Parasitoids, Predators and PCR: The Use of Diagnostic Molecular Markers in Biological Control of Arthropods. Journal of Applied Entomology, 131, 225-240. http://dx.doi.org/10.1111/j.1439-0418.2007.01145.x
[9] Castalanellia, M.A., Severtsonc, D.L., Brumleya, C.J., Szitoa, A., Foottitd, R.G., Grimma, M., Munyardb, K. and Grothb, D.M. (2010) A Rapid Non-Destructive DNA Extraction Method for Insects and Other Arthropods. Journal of Asia-Pacific Entomology, 3, 243-248.
[10] Tian, E.-W. and Yu, H. (2013) A Simple and Rapid DNA Extraction Protocol of Small Insects for PCR Amplification. Entomological News, 123, 303-310. http://dx.doi.org/10.3157/021.123.0403
[11] DNA Extraction-Salting out Protocol (2009) Open Wet Ware.
[12] ChargeSwitch® Forensic DNA Purification Kit. (n.d.)
[13] PrepGEMTM DNA Extraction-Blood. (n.d.).
[14] Gilbert, M.T.P., Moore, W., Melchior, L. and Worobey, M. (2007) DNA Extraction from Dry Museum Beetles without Conferring External Morphological Damage. PLoS ONE, 2.
[15] Chen, H., Rangasamy, M., Tan, S.Y., Wang, H. and Siegfried, B.D. (2010) Evaluation of Five Methods for Total DNA Extraction from Western Corn Rootworm Beetles. PLoS ONE, 5.
[16] Gentra Systems (2004) Puregene® Genetic DNA Purification Kit Instructions.
[17] Molecular Research Center (2008) DNAzol® Genomic DNA Isolation Reagent.
[18] Quagen (2006) DNeasy® Blood and Tissue Handbook.
[19] Henry, J.M., Raina, A.K. and Ridgway, R.L. (1990) Isolation of High-Molecular-Weight DNA from Insects. Analytical Biochemistry, 185, 147-150. http://dx.doi.org/10.1016/0003-2697(90)90270-J

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