Potential of Terpenoid Bioactive Compound Isolated from Papua Ant Nest as an Alternative Ovarian Cancer Treatment


Objectives: The purpose of this study was to determine anticancer activity of terpenoid bioactive compound isolated from Papua ant nest on ovarian cancer cells in vitro. Methods: This was a laboratory experimental study which aims to determine the potential of the terpenoid bioactive compound isolated from Papua ant nest to inhibit the growth and induce apoptotic process on ovarian cancer cells (SKO-3) in vitro. Result: Terpenoid had capability to inhibit the growth of ovarian cancer cell line (SKO-3) in vitro, with IC50 of 481 ug/ml at 48 hours and 463 ug/ml at 48 hours, respectively. At a concentration of 600 ug/ml, terpenoid was able to induce apoptotic process on ovarian cancer cell lines in vitro with the apoptotic index of 30% at 24 hours, 35% at 48 hours and 37% at 72 hours, respectively. Conclusion: Terpenoid bioactive compound isolated from Papua ant nest had the ability to inhibit the growth and was able to induce apoptotic process on ovarian cancer cell lines (SKO-3) in vitro.

Share and Cite:

Hasanuddin, Rifayani, K., Supriadi, G., Kurnia, D. and Adhita, D. (2015) Potential of Terpenoid Bioactive Compound Isolated from Papua Ant Nest as an Alternative Ovarian Cancer Treatment. Open Journal of Obstetrics and Gynecology, 5, 406-411. doi: 10.4236/ojog.2015.57058.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Kozlowska, E., Makowska, H., Zadrozny, M., Mankiewicz, J., Szyllo, K., Pertynski, T., et al. (2003) Detection and Analysis of Apoptosis in Breast and Ovarian Cancer Subjects—Genetic Studies. Polish Journal of Pathology, 54, 25-29.
[2] Ferlay, J., Shin, H. and Bray, F. (2010) Estimates of Worldwide Burden of Cancer in 2008: GLOBOCAN 2008. International Journal of Cancer, 127, 2893-2917.
[3] Williams, T.I., Toups, K.L., Saggese, D.A., Kalli, K.R., Cliby, W.A. and Muddiman, D.C. (2007) Epithelial Ovarian Cancer: Disease Etiology, Treatment, Detection, and Investigational Gene, Metabolite, and Protein Biomarkers. Journal of Proteome Research, 6, 2936-2962.
[4] Mok, S.C., Kwong, J., Welch, W.R., Samimi, G., Ozbun, L., Bonome, T., et al. (2007) Etiology and Pathogenesis of Epithelial Ovarian Cancer. Disease Markers, 23, 367-376.
[5] Wong, R.S.Y. (2011) Apoptosis in Cancer: From Pathogenesis to Treatment. Journal of Experimental & Clinical Cancer Research, 30, 1-14.
[6] Faried, A., Kurnia, D., Faried, L.S., Usman, N., Miyazaki, T., Kato, H., et al. (2007) Anticancer Effects of Gallic Acid Isolated from Indonesian Herbal Medicine, Phaleria macrocarpa (Scheff) Boerl, on Human Cancer Cell Lines. International Journal of Oncology, 30, 605-613.
[7] Partridge, E.E. and Barnes, M. (1999) Epithelial Ovarian Cancer: Prevention, Diagnosis, and Treatment. CA: A Cancer Journal for Clinicians, 49, 297-320.
[8] Sugarbaker, P.H. (2009) Cytoreductive Surgery and Perioperative Intraperitoneal Chemotherapy for the Treatment of Advanced Primary and Recurrent Ovarian Cancer. Current Opinion in Obstetrics and Gynecology, 21, 15-24.
[9] Taraphdar, A.K., Roy, M. and Bhattacharya, R.K. (2001) Natural Products as Inducer of Apoptosis: Implication for Cancer Therapy and Prevention. Current Science, 80, 1-10.
[10] Lu, J.J., Dang, Y., Huang, M., Xu, W., Chen, X. and Wang, Y. (2012) Anti-Cancer Properties of Terpenoids Isolated from Rhizoma Curcumae—A Review. Journal of Ethnopharmacology, 143, 406-411.
[11] Ghavami, S., Hashemi, M., Ande, S.R., Yeganeh, B., Xiao, W., Eshraghi, M., et al. (2009) Apoptosis and Cancer: Mutations within Caspase Genes. Journal of Medical Genetics, 46, 497-510.
[12] Matsumoto,Y., Iwasaka, T., Yamasaki, F. and Sugimori, H. (1999) Apoptosis and Ki-67 Expression in Adenomyotic Lesions and in the Corresponding Eutopic. Obstetrics and Gynecology, 94, 71-77.
[13] Hamsar, M.N. and Mizaton, H.H. (2012) Potential of Ant-Nest Plant as Alternative Cancer Treatment. Journal of Pharmaceutical Sciences, 5, 3063-3066.
[14] Hasmah, A., Hohmann, J., Azimahtol, L.P., Molnar, J. and Forgo, P. (2008) Antiproliferative Compounds from Hypnophytum fornicarum. Journal of Tropical Medicinal Plants, 9, 366-371.
[15] Hertiani, T., Sasmito, E., Sumardi and Ulfah, M. (2010) Preeliminary Study on Immunomodulatory Effect of Sarang Semut Myrmecodia tuberosa and Myrmecodia pendans. OnLine Journal of Biological Sciences, 10, 136-141.
[16] Soeksmanto, A., Subroto, M.A., Wijaya, H. and Simanjuntak, P. (2010) Anticancer Activity Test for Extracts of Sarang Semut Plant (Myrmecodya pendens) to HeLa and MCM-B2 cells. Pakistan Journal of Biological Sciences, 13, 148-151.
[17] Darsono, H.D.A. (2013) Penelitian awal isolat sarang semut Papua. Universitas Padjadjaran, Bandung.
[18] Shibata, S. (2001) Chemistry and Cancer Preventing Activities of Ginseng Saponins and Some Related Triterpenoids Compounds. Journal of Korean Medical Science, 16, S28-S37.
[19] Sismindari, R., Putu Indah, I.A. and Yulina Enstini, N.L.P. (2009) Apoptotic Induction of Ribosome-Inactivating Proteins (RIPs) Isolated from Carfica papaya L. Leaves on Cancer Cell-Lines. Majalah Farmasi Indonesia, 20, 9-16.
[20] Lage, H., Duarte, N., Coburger, C., Hilgeroth, A. and Ferreira, M.J.U. (2010) Antitumor Activity of Terpenoids against Classical and Atypical Multidrug Resisteant Cancer Cells. Phytom Edicinea, 17, 441-448.
[21] Daponte, A., Kostopoulou, E., Minas, M., Koukoulis, G. and Messinis, I.E. (2008) Prognostic Significance of Fascin Expression in Advanced Poorly Differentiated Serous Ovarian Cancer. Anticancer Research, 28, 1905-1910.
[22] Fatmawati, D., Puspitasari, P.K. and Yusuf, I. (2011) Efek sitotoksik ekstrak etanol sarang semut (Myrmecodia pendens) pada sel line kanker serviks HeLa. Sains Medika, 3, 117-120.
[23] Subroto, M.A., Saputro, H. (2006)Gempur penyakit dengan sarang semut. Penebar Swadaya, Depok, 15-31.
[24] Cao, W., Li, X.Q., Wang, X., Fan, H.T, Zhang, X.N., Hou, Y., et al. (2010) A Novel Polysaccharide, Isolated from Angelica sinensis (Oliv) Diels Induces the Apoptosis of Cervical Cancer HeLa through an Intrinsic Apoptotic Pathway. Phytomedicine, 17, 598-605.
[25] Chen, Z., Naito, M., Mashima, T., Hiroyuki, S. and Tsuruo, T. (2001) Activation of Caspase Protease during Apoptosis in Ovarian Cancer Cells. Methods in Molecular Medicine, 39, 681-685.
[26] Li, K., Li, Q., Han, Z., Li, J., Gao, D., Liu, Z., et al. (2008) Alkaloid from Angelicae dahuricae Inhibit HeLa Cell Growth by Inducing Aoptosis and Increasing Caspase-3 Activity. Labmedicine, 39, 540-546.

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.