Use of Graphene and Cucurbit[7]uril Electrodes for the Determination of Amantadine in Biological Fluids


A differential pulse voltammetry (DPV) method for amantadine (AT) determination is developed. To this end, all the chemical and instrumental variables affecting the determination of amantadine are optimized. These studies have used three types of glassy-carbon electrode, first electrode which has not undergone surface modification or coating, to then modify the working electrode surface with two kinds of suspensions: graphene and graphene-cucurbit[7]uril (CB[7]). From studies of the mechanisms governing the electrochemical response of amantadine, it was concluded that it is an electrochemically system with a diffusive reduction phenomenon. Under optimal conditions and with the appropriate electrode modification, we proceed to study the relation between the peak intensity with the analyte concentration. Thus, we find that when the electrode surface is covered with graphene-CB[7], two linear sections are obtained, the first one in the concentration range of between 0.05 μg·mL﹣1 and 0.75 μg·mL﹣1 ; and the second one between 1.00 μg·mL﹣1 and 6.00 μg·mL﹣1, with Er (%) = 87 and R.S.D. = 0.94% (n = 10 at 0.5 μg·mL﹣1 level). The minimum detectable amount was 15 ng·mL﹣1 while a concentration of 44 ng·mL﹣1 was calculated as determination limit. The optimized method was applied to the determination of amantadine in biological fluids.

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Domínguez, C. and Hernández, P. (2015) Use of Graphene and Cucurbit[7]uril Electrodes for the Determination of Amantadine in Biological Fluids. American Journal of Analytical Chemistry, 6, 623-630. doi: 10.4236/ajac.2015.67060.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Cui, S.J., Feng, F., Lui, H. and Ma, M. (2007) New Method for High-Performance Liquid Chromatographic Determination of Amantadine and Its Analogues in Rat Plasma. Journal of Pharmaceutical and Biomedical Analysis, 44, 1100- 1105.
[2] Wang, P., Liang, Y.-Z., Chen, B.-M., Zhou, N., Yu, Y. and Yi, Z.-B. (2007) Quantitative Determination of Amantadine in Human Plasma by Liquid Chromatography-Mass Spectrometry and the Application in a Bioequivalence Study. Journal of Pharmaceutical and Biomedical Analysis, 43, 1519-1525.
[3] Arndt, T., Guessregen, B., Hohl, A. and Reis, J. (2005) Determination of Serum Amantadine by Liquid Chromatography-Tandem Mass Spectrometry. Clinica Chimica Acta, 359, 125-131.
[4] Duh, T.-H., Wu, H.-L., Pan, C.-W. and Kou, H.-S. (2005) Fluorimetric Liquid Chromatographic Analysis of Amantadine in Urine and Pharmaceutical Formulation. Journal of Chromatography A, 1088, 175-181.
[5] Bai, G.-R., Chittaganpitch, M., Kanai, Y., Li, Y.-G., Auwanit, W., Ikuta, K. and Sawanpanyalert, P. (2009) Amantadine- and Oseltamivir-Resistant Variants of Influenza A Viruses in Thailand. Biochemical and Biophysical Research Communications, 390, 897-901.
[6] Cersosimo, M.G., Scorticati, M.C. and Micheli, F.E. (2000) Amantadine for the Treatment of Levodopa-Induced Dyskinesias in Parkinson’s Disease. Medicina (Buenos Aires), 60, 321-325.
[7] Singer, C., Papapatropoulos, S., Uzcategui, G. and Vela, L. (2006) The Use of Amantadine HCL in Clinical Practice: A Study of Old and New Indications. The Journal of Applied Research, 6, 240-245.
[8] Lee, C.G., Wei, X.D., Kyser, J.W. and Hone, J. (2008) Measurement of the Elastic Properties and Intrinsic Strength of Monolayer Graphene. Science, 321, 385-388.
[9] Wallace, P.R. (1947) The Band Theory of Graphite. Physical Review, 71, 622.
[10] Guo, Y.J., Wen, D., Zhai, Y.M., Dong, S.J. and Wang, E.K. (2010) Platinum Nanoparticle Ensemble-on-Graphene HYBRID nanosheet: One-Pot, Rapid Synthesis, and Used as New Electrode Material for Electrochemical Sensing. ACS Nano, 4, 3959-68.
[11] Peres, N.M.R. (2010) Colloquium: The Transport Properties of Graphene: An Introduction. Reviews of Modern Physics, 82, 2673-2700.
[12] Lagona, J., Mukhopadhyay, P., Chakrabarti, S. and Isaacs, L. (2005) Review: The Cucurbit[n]uril Family. Angewandte Chemie International Edition, 44, 4844.
[13] Kim, K., Selvapalam, N., Ko, Y.H., Park, K.M., Kim, D. and Kim. J. (2007) Functionalized Cucurbiturils and Their Applicvations. Chemical Society Reviews, 36, 267.
[14] Behrend, R., Meyer, E. and Rusche, F. (1905) über Condensation-producte aus Glycoluril und Formaldehyd. Justus Liebigs Annalen der Chemie, 339, 1.
[15] Liu, S.M., Ruspic, C., Mukhopadhyay, P., Chakrabarti, S., Zavalij, P.Y. and Isaacs, L. (2005) The Cucurbit[n]uril Family: Prime Components for Self-Sorting Systems. Journal of the American Chemical Society, 127, 15959-15967.
[16] Masson, E., Ling, X.X., Joseph, R., Kyeremeh-Mensah, L. and Lu, X.Y. (2012) Cucurbituril Chemistry: A Tale of Supramolecular Success. RSC Advances, 2, 1213-1247.
[17] Maha, A.S. (2004) Spectrophotometric Determination of Amantadine in Dosage Forms. Current Topics in Analytical Chemistry, 4, 103-109.

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