Oxidative Stress Biomarkers Response to Exercise in Brazilian Junior Soccer Players


The purpose was to analyze biomarkers of oxidative stress and muscle damage in junior soccer players undergoing intermittent exercise to verify the biochemical changes. Ten trained, healthy male soccer players (age 18.3 ± 0.7 years, body mass 74.3 ± 7.4 kg, height 175.5 ± 6.7 cm, body mass index 24.14 ± 1.15 kg/m2; mean ± SD) from the junior cate-gory of an elite Brazilian football association participated in this study. They accomplished a running test (Loughbo-rough Intermittent Shuttle Test) which simulates common soccer activity patterns. Blood samples were collected before, during and immediately after the exercise for glucose, lactate, creatinine, urea, ascorbic acid, total plasma antioxidant potential, lipid hydroperoxides, malondialdehyde and creatine kinase concentrations. During the exercise no changes were observed in biomarkers, but, immediately after there was a significant decrease in total plasma antioxidant potential (ranging from 650.37 ± 66.53 µmol.L-1 to 559.95 ± 91.38 µmol.L-1, p < 0.05) while malondialdehyde and creatine kinase had increased (ranging, respectively, from 6.69 ± 0.81 µmol.L-1 to 8.35 ± 0.83 nmol.L-1 and from 272.01 ± 49.67 U.I.?L-1 to 304.65 ± 39.13 U.I.?L-1, p < 0.05). Findings suggest that the exercise protocol induced significant changes in oxidative stress selected biomarkers in the early stage of recovery. Thus, taking into account that soccer players’ competitive careers initiate very precociously being under constant physical exhaustion, further research on junior soccer players’ physiology and health are important for more effective physical and nutritional programming.

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C. Costa, M. Barbosa, J. Spineti, C. Pedrosa and A. Pierucci, "Oxidative Stress Biomarkers Response to Exercise in Brazilian Junior Soccer Players," Food and Nutrition Sciences, Vol. 2 No. 5, 2011, pp. 407-413. doi: 10.4236/fns.2011.25057.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] J. Hoff and J. Helgerud, “Endurance and Strength Training for Soccer Players—Physiological Considerations,” Sports Medicine, Vol. 34, No. 3, 2004, pp. 165-180. doi:10.2165/00007256-200434030-00003
[2] T. St?len, K. Chamari, C. Castagna and U. Wisl?ff, “Physiology of Soccer: An Update,” Sports Medicine, Vol. 35, No. 6, 2005, pp. 501-536.
[3] J. Str?yer, L. Hansen and K. Klausen, “Physiological Profile and Activity Pattern of Young Soccer Players during Match Play,” Medicine and Science in Sports and Exercise, Vol. 36, No. 1, 2004, pp. 168-174. doi:10.1249/01.MSS.0000106187.05259.96
[4] J. Helgerud, L. C. Engen, U. Wisl?ff and J. Hoff, “Aerobic Endurance Training Improves Soccer Performance,” Medicine and Science in Sports and Exercise, Vol. 33, No. 11, 2001, pp. 1925-1931. doi:10.1097/00005768-200111000-00019
[5] C. B. Wragg, N. S. Maxwell and J. H. Doust, “Evaluation of the Reliability and Validity of a Soccer-Specific Field Test of Repeated Sprint Ability,” European Journal of Applied Physiology, Vol. 83, No. 1, 2000, pp. 77-83. doi:10.1007/s004210000246
[6] N. B. Vollaard, J. P. Shearman and C. E. Cooper, “Exercise-Induced Oxidative Stress: Myths, Realities and Physiological Relevance,” Sports Medicine, Vol. 35, No. 12, 2005, pp. 1045-1062. doi:10.2165/00007256-200535120-00004
[7] M. L. Urso and P. M. Clarkson, “Oxidative Stress, Exercise, and Antioxidant Supplementation,” Toxicology, Vol. 189, No. 1-2, 2003, pp. 41-54. doi:10.1016/S0300-483X(03)00151-3
[8] J. Finaud, G. Lac and E. Filaire, “Oxidative Stress: Relationship with Exercise and Training,” Sports Medicine, Vol. 36, No. 4, 2006, pp. 327-358. doi:10.2165/00007256-200636040-00004
[9] C. K. Sen, “Antioxidant and Redox Regulation of Cellular Signaling: Introduction,” Medicine and Science in Sports and Exercise, Vol. 33, No. 3, 2001, pp. 368-370. doi:10.1097/00005768-200103000-00005
[10] A. Ascens?o, A. Rebelo, E. Oliveira, F. Marques, L. Pereira and J. Magalh?es, “Biochemical Impact of a Soccer Match—Analysis of Oxidative Stress and Muscle Damage Markers throughout Recovery,” Clinical Biochemistry, Vol. 41, No. 10-11, 2008, pp. 841-851. doi:10.1016/j.clinbiochem.2008.04.008
[11] J. Magalh?es, A. Rebelo, E. Oliveira, J. R. Silva, F. Marques and A. Ascens?o, “Impact of Loughborough Intermittent Shuttle Test Versus Soccer Match on Physiological, Biochemical and Neuromuscular Parameters,” European Journal of Applied Physiology, Vol. 108, No. 1, 2010, pp. 39-48. doi:10.1007/s00421-009-1161-z
[12] J. Cholewa, S. Poprzecki, A. Zajac, and Z. Waskiewicz, “The Influence of Vitamin C on Blood Oxidative Stress Parameters in Basketball Players in Response to Maximal Exercise,” Science & Sports, Vol. 23, No. 3-4, 2008, pp. 176-182. doi:10.1016/j.scispo.2008.01.004
[13] A. Mastaloudis, S. W. Leonard and M. G. Traber, “Oxidative Stress in Athletes during Extreme Endurance Exercise,” Free Radical Biology and Medicine, Vol. 37, No. 7, 2001, pp. 911-922. doi:10.1016/S0891-5849(01)00667-0
[14] A. Mastaloudis, J. D. Morrow, D. W. Hopkins, S. Devaraj and M. G. Traber, “Antioxidant Supplementation Prevents Exercise-Induced Lipid Peroxidation, But Not Inflammation, in Ultramarathon Runners,” Free Radical Biology and Medicine, Vol. 36, No. 10, 2004, pp. 1329- 1341. doi:10.1016/j.freeradbiomed.2004.02.069
[15] C. W. Nicholas, F. E. Nuttall and C. Williams, “The Loughborough Intermittent Shuttle Test: A Field Test That Simulates the Activity Pattern of Soccer,” Journal of Sports Sciences, Vol. 18, No. 2, 2000, pp. 97-104. doi:10.1080/026404100365162
[16] C. W. Nicholas, K. Tsintzas, L. Boobis and C. Williams, “Carbohydrate-Electrolyte Ingestion during Intermittent High-Intensity Running,” Medicine and Science in Sports and Exercise, Vol. 31, No. 9, 1999, pp. 1280-1286. doi:10.1097/00005768-199909000-00008
[17] S. D. Patterson and S. C. Gray, “Carbohydrate-Gel Supplementation and Endurance Performance during Intermittent High-Intensity Shuttle Running,” International Journal of Sport Nutrition and Exercise Metabolism, Vol. 17, No. 5, 2007, pp. 445-455.
[18] D. Thompson, C. Williams, M. Kingsley, C. W. Nicholas, H. K. Lakomy, F. McArdle and M. J. Jackson, “Muscle Soreness and Damage Parameters after Prolonged Intermittent Shuttle-Running Following Acute Vitamin C Supplementation,” International Journal of Sports Medicine, Vol. 22, No. 1, 2001, pp. 68-75. doi:10.1055/s-2001-11358
[19] D. Thompson, E. D. Bailey, E. J. Hill, E. T. Hurst, J. R. Powell and E. C. Williams, “Prolonged Vitamin C Supplementation and Recovery from Eccentric Exercise,” European Journal of Applied Physiology, Vol. 92, No. 1-2, 2004, pp. 133-138. doi:10.1007/s00421-004-1064-y
[20] A. S. Jackson and M. L. Pollock, “Generalized Equations for Predicting Body Density of Men,” The British Journal of Nutrition, Vol. 40, No. 3, November 1978, pp. 497-504. doi:10.1079/BJN19780152
[21] L. A. Léger and J. Lambert, “A Maximal Multistage 20-m Shuttle Run Test to Predict VO2 Max,” European Journal of Applied Physiology and Occupational Physiology, Vol. 49, No. 1, 1982, pp. 1-12. doi:10.1007/BF00428958
[22] N. R. Rodriguez, N. M. DiMarco, S. Langley, American Dietetic Association, Dietetians of Canada and American College of Sports Medicine, “Position of the American Dietetic Association, Dietitians of Canada, and the American College of Sports Medicine: Nutrition and Athletic Performance,” Journal of the American Dietetic Association, Vol. 109, No. 3, 2009, pp. 509-527. doi:10.1016/j.jada.2009.01.005
[23] E. Bobrowicz, J. W. Naskalski and A. Siedlecki, “Preanalytical factors in human plasma ascorbate assay,” Clinical Chimica Acta, Vol. 314, No. 1-2, 2001, pp. 237-239. doi:10.1016/S0009-8981(01)00577-0
[24] E. S?dergren, J. Nourooz-Zadeh, L. Berglund and B. Vessby, “Re-Evaluation of the Ferrous Oxidation in Xylenol Orange Assay for the Measurement of Plasma Lipid Hydroperoxides,” Journal of Biochemical and Biophysi- cal Methods, Vol. 37, No. 3, 1998, pp. 137-146. doi:10.1016/S0165-022X(98)00025-6
[25] J. Nourooz-Zadeh, J. Tajaddini-Sarmadi, S. P. Wolff, “Measurement of Plasma Hydroperoxide Concentrations by the Ferrous Oxidation-Xylenol Orange Assay in Con- juncttion with Triphenylphosphine,” Analytical Bio-che- mistry, Vol. 220, No. 2, 1994, pp. 403-409. doi:10.1006/abio.1994.1357
[26] F. Karatas, M. Karatepe and A. Baysar, “Determination of Free Malondialdehyde in Human Serum by High-Performance Liquid Chromatography,” Analytical Biochemis- try, Vol. 311, No. 1, 2002, pp. 76-79. doi:10.1016/S0003-2697(02)00387-1
[27] I. F. Benzie and J. J. Strain, “The Ferric Ability of Plasma (FRAP) as a Measure of ‘antioxidant power’: The FRAP Assay,” Analytical Biochemistry, Vol. 239, No. 1, July 1996, pp. 70-76. doi:10.1006/abio.1996.0292
[28] M. Mohr, P. Krustrup and J. Bangsbo, “Match Performance of High-Standard Soccer Players with Special Reference to Development of Fatigue,” Journal of Sports Sciences, Vol. 21, No. 7, 2003, pp. 519-528. doi:10.1080/0264041031000071182
[29] P. Krustrup, M. Mohr, A. Steensberg, J. Bencke, M. Kjaer and J. Bangsbo, “Muscle and Blood Metabolites during a Soccer Game: Implications for Sprint Performance,” Medicine and Science in Sports and Exercise, Vol. 38, No. 6, 2006, pp. 1165-1174. doi:10.1249/01.mss.0000222845.89262.cd
[30] C. C. Zoppi, R. Hohl, F. C. Silva, F. L. Lazarim, J. M. Neto, M. Stancanneli and D. V. Macedo, “Vitamin C and E Supplementation Effects in Professional Soccer Players under Regular Training,” Journal of the International Society of Sports Nutrition, Vol. 3, 2006, pp. 37-44. doi:10.1186/1550-2783-3-2-37
[31] S. R. McAnulty, L. S. McAnulty, D. C. Nieman, J. D. Morrow, L. A. Shooter, S. Holmes, C. Heward and D. A. Henson, “Effect of Alpha-Tocopherol Supplementation on Plasma Homocysteine and Oxidative Stress in Highly Trained Athletes before and after Exhaustive Exercise,” The Journal of Nutritional Biochemistry, Vol. 16, No. 9, 2005, pp. 530-537. doi:10.1016/j.jnutbio.2005.02.001

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