Optimization of Mycelial Biomass Production in Submerged Culture Fermentation of Pleurotus flabellatus Using Response Surface Methodology


The factors selected to optimize the productivity of Pleurotus flabellatus biomass in 250ml working volume Erlenmeyer flask were agitation rate, initial pH value and incubation temperature. The central composite design was applied to study the significant factors and the interactions between the chosen factors, if present. The Design Expert software generated 20 runs. The optimized conditions obtained were as follows: the agitation rate of 129.8 rpm, incubation temperature at 27.8°C, and initial pH of 6.06. The optimized conditions tripled the productivity at the range of 980 - 1040 mg/litre/day compared to the initial rate productivity at 310 mg/litre/day. From the quadratic equation,the agitation rate, temperature and the interaction between agitation rate and temperature were found to be significant (p < 0.05). At optimum conditions, the experimental data supported the theoretical estimate.

Share and Cite:

Mohamad, S. , Awang, M. , Rashid, R. , Ling, L. , Daud, F. , Hamid, A. , Ahmad, R. and Wan Yusoff, W. (2015) Optimization of Mycelial Biomass Production in Submerged Culture Fermentation of Pleurotus flabellatus Using Response Surface Methodology. Advances in Bioscience and Biotechnology, 6, 419-426. doi: 10.4236/abb.2015.66041.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Wasser, S.P. (2002) Medicinal Mushrooms as a Source of Antitumor and Immunomodulating Polysaccharides. Applied Microbiology and Biotechnology, 60, 258-274.
[2] Zhang, M., Cui, S.W., Cheung, P.C.K. and Wang, Q. (2007) Antitumor Polysaccharides from Mushrooms: A Review on Their Isolation Process, Structural Characteristics and Antitumor Activity. Trends in Food Science & Technology, 18, 4-19.
[3] Park, J.P., Kim, Y.M., Kim, S.W., Hwang, H.J., Cho, Y.J., Lee, Y.S., Song, C.H. and Yun, J.W. (2002) Effect of Agitation Intensity on the Exo-Biopolymer Production and Mycelia Morphology in Cordyceps militaris. Letters in Applied Microbiology, 34, 433-438.
[4] Shih, I.L., Tsai, K.L. and Hsieh, C. (2007) Effects of Culture Conditions on the Mycelia Growth and Bioactive Metabolite Production in Submerged Culture of Cordyceps militaris. Biochemical Engineering Journal, 33, 193-201.
[5] Lee, B.C., Bae, J.T., Pyo, H.B., Choe, T.B., Kim, S.W., Hwang, H.J. and Yun, J.W. (2004) Submerged Culture Conditions for the Production of Mycelial Biomass and Exopolysaccharides by the Edible Basidiomycete Grifola frondosa. Enzyme and Microbial Technology, 35, 369-376.
[6] Stamets, P. (2000) Growing Gourmet and Medicinal Mushrooms. 3rd Edition, Ten Speed Press, Berkeley.
[7] Mehta, K.B. and Bhandal, M.S. (1988) Mycelial Growth Variation of Six Pleurotus Species at Different Temperatures. Indian Journal of Mushroom, 14, 64-65.
[8] Kashangura, C., Hallsworth, J.E. and Mswaka, A.Y. (2006) Phenotypic Diversity amongst Strains of Pleurotus sajor Caju: Implications for Cultivation in Arid Environments. Mycological Research, 110, 312-317.
[9] Lakshmi, S.S. (2013) Comparative Study on Mycelia Growth Rate of Ganoderma lucidum and Pleurotus flabellatus on Agro-Wastes. International Journal of Advanced Research, 1, 199-203.
[10] Islam, M.Z., Rahman, M.H. and Hafiz, F. (2009) Cultivation of Oyster Mushroom (Pleurotus flabellatus) on Different Substrates. International Journal of Sustainable Crop Production, 4, 45-48.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.