Fluorescence Emission Spectrum of Elodea Leaves Exposed to Nanoparticles

DOI: 10.4236/jbnb.2015.63013   PDF   HTML     5,084 Downloads   5,770 Views   Citations


The intensive use of engineered nanoparticles (NPs) in industrial, agricultural and household applications will very likely lead to the release of such materials into the environment, especially water ecosystems. Water plants are an integral part of ecosystems; hence their interaction with NPs is inevitable. It is important to understand the consequences of this interaction and assess its potential effects. There are different types of approaches for investigating the toxic effects of NPs on plants. Chlorophyll fluorescence (ChlF) is one of interesting biophysical methods for testing the effects NPs on plants in vivo. ChlF is a suitable technique and a very powerful tool for the in vivo studying of photochemical and non-photochemical processes within thylakoid membranes, chloroplasts, plant tissues, and whole plants. The present work reports the in vivo observation of chlorophyll a fluorescence quenching induced by the iron (Fe3O4, Fe2O3) and aluminum oxide (Al2O3) nanoparticles. Excitation and emission spectra of intact leaves of Elodea were acquired by fluorescence spectrophotometer (Cary Eclipse) at room temperature. It was shown that the intensity of the ChlF decreased in the solution of Fe3O4 and Al2O3 nanoparticles on the light. Fe2O3 affected slightly and the toxicity of nanoparticles depended on dose and exposure period. It was clear from these experiments that the given nanoparticles penetrated into the cell and might decrease the chlorophyll content of leaves.

Share and Cite:

Maharramov, A. , Ahmadov, I. , Ramazanov, M. , Aliyeva, S. and Ramazanli, V. (2015) Fluorescence Emission Spectrum of Elodea Leaves Exposed to Nanoparticles. Journal of Biomaterials and Nanobiotechnology, 6, 135-143. doi: 10.4236/jbnb.2015.63013.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Rai, P.K. (2009) Heavy Metal Phytoremediation from Aquatic Ecosystems with Special Reference to Macrophytes. Critical Reviews in Environmental Science and Technology, 39, 697-753.
[2] Stampoulis, D. Sinha, S.K. and White, J.C. (2009) Assay-Dependent Phytotoxicity of Nanoparticles to Plants. Environmental Science and Technology, 43, 9473-9479.
[3] Arvizo, R.R., Miranda, O.R., Thompson, M.A., Pabelick, C.M., Bhattacharya, R., Robertson, J.D., Rotello, V.M., Prakash, Y.S. and Mukherjee, P. (2010) Effect of Nanoparticle Surface Charge at the Plasma Membrane and Beyond. Nano Letters, 10, 2543-2548.
[4] Leroueil, P.R., Berry, S.A., Duthie, K., Han, G., Rotello, V.M., McNerny, D.Q., Baker, J.R., Orr, B.G. and Holl, M.M.B. (2008) Wide Varieties of Cationic Nanoparticles Induce Defects in Supported Lipid Bilayers. Nano Letters, 8, 420-424.
[5] Atha, D.H., Wang, H.H., Petersen, E.J., Cleveland, D., Holbrook, D.R., Jaruga, P. et al. (2012) Copper Oxide Nanoparticle Mediated DNA Damage in Terrestrial Plant Models. Environmental Science and Technology, 46, 1819-1827.
[6] Dewez, D. and Oukarroum, A. (2012) Silver Nanoparticles Toxicity Effect on Photosystem II Photochemistry of the Green Alga Chlamydomonas reinhardtii Treated in Light and Dark Conditions. Toxicological & Environmental Chemistry, 94, 1536-1546.
[7] Santos, A.R., Miguel, A.S., Tomaz, L., Malhó, R., Maycock, C., Vaz Patto, M.C., Fevereiro, P. and Oliva, A. (2010) The Impact of CdSe/ZnS Quantum Dots in Cells of Medicago sativa in Suspension Culture. Journal of Nanobiotechnology, 8, 24.
[8] Lu, C.M., Zhang, C.Y., Wen, J.Q., Wu, G.R. and Tao, M.X. (2002) Research of the Effect of Nanometer Materials on Germination and Growth Enhancement of Glycine max and Its Mechanism. Soybean Science, 21, 168-171.
[9] Hak, R., Lichtenthaler, H.K. and Rinderle, U. (1990) Decrease of the Fluorescence Ratio F690/F730 during Greening and Development of Leaves. Radiation and Environmental Biophysics, 29, 329-336.
[10] Lichtenthaler, H.K., Hak, R. and Rinderle, U. (1990) The Chlorophyll Fluorescence Ratio F690/F730 in Leaves of Different Chlorophyll Content. Photosynthesis Research, 25, 295-298.
[11] Lichtenthaler, H.K., Stober, F. and Lang, M. (1992) The Nature of the Different Laser-Induced Fluorescence Signatures of Plants. EARSeL Advances in Remote Sensing, 1, No. 2-II.
[12] Juneau, P. and Popovic, R. (1999) Evidence for the Rapid Phytotoxicity and Environmental Stress Evaluation Using the PAM Fluorometric Method: Importance and Future Application. Ecotoxicology, 8, 449-455.
[13] Baker, N.R. (2008) Chlorophyll Fluorescence: A Probe of Photosynthesis in Vivo. Annual Review of Plant Biology, 59, 89-113.
[14] Johnson, M.E., Ostroumov, S.A., Tyson, J.F. and Xing, B. (2011) Study of the Interactions between Elodea canadensis and CuO Nanoparticles. Russian Journal of General Chemistry, 81, 2688-2693.
[15] Lichtenthaler, H.K. and Rinderle, U. (1988) The Role of Chlorophyll Fluorescence in the Detection of Stress Conditions in Plants. CRC Critical Reviews in Analytical Chemistry, 19, S29-S85.
[16] Rinderle, U. and Lichtenthaler, H.K. (1988) The Chlorophyll Fluorescence Ratio F690/F735 as Possible Stress Indicator. In: Lichtenthaler, H.K. Applications of Chlorophyll Fluorescene in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing, Springer Netherlands, City name, 189-196.
[17] Zhu, H., Han, J., Xiao, J.Q. and Jin, Y. (2008) Uptake, Translocation, and Accumulation of Manufactured Iron Oxide Nanoparticles by Pumpkin Plants. Journal of Environmental Monitoring, 10, 713-717.
[18] Wilson, M.R., Lightbody, J.H., Donaldson, K., Sales, J. and Stone, V. (2002) Interactions between Ultrafine Particles and Transition Metals in Vivo and in Vitro. Toxicology and Applied Pharmacology, 184, 172-179.
[19] Gonzalez-Melendi, P., Fernández-Pacheco, R., Coronado, M.J., et al. (2008) Nanoparticles as Smart Treatment Delivery Systems in Plants: Assessment of Different Techniques of Microscopy for Their Visualization in Plant Tissues. Annals of Botany, 101, 187-195.
[20] Corredor, E., Testillano, P.S., Coronado, M.-J., et al. (2009) Nanoparticle Penetration and Transport in Living Pumpkin Plants: In Situ Sub Cellular Identification. BMC Plant Biology, 9, 45.
[21] Spori, C.L., Prigent, G., Schaer, M., Crittin, M., Matus, P., Laroche, T., Sikora, B., Kaminska, I., Fronc, K., Elbaum, D., Digigow, R., Fink, A., Ahmadov, I., Khalilov, R., Ramazanov, M., Forró, L. and Sienkiewicz, A. (2014) Uptake and Biomagnification of Multifunctional Magnetic and NIR Sensitive Nanoparticles by NIR-Aquatic Plants: Electron Spin Resonance, Twophoton and Confocal Microscopy Studies. Proceedings of the Nano-Tera Annual Plenary Meeting, Lausanne, 19-20 May 2014, 82.
[22] Falco, W.F., Botero, E.R., Falco, E.A., Santiag, E.F., Bagnato, V.S. and Caires, A.R.L. (2011) In Vivo Observation of Chlorophyll Fluorescence Quenching Induced by Gold Nanoparticles. Journal of Photochemistry and Photobiology A: Chemistry, 225, 65-71.
[23] Ursache-Oprisan, M., Focanici, E., Creanga, D. and Caltun, O. (2011) Sunflower Chlorophyll Levels after Magnetic Nanoparticle Supply. African Journal of Biotechnology, 10, 7092-7098.
[24] Jiang, H.-S., Li, M., Chang, F.-Y., Li, W. and Yin, L.-Y. (2012) Physiological Analysis of Silver Nanoparticles and AgNO3 Toxicity to Spirodela polyrhiza. Environmental Toxicology and Chemistry, 31, 1880-1886.
[25] Dash, A., Singh, A.P., Chaudhary, B.R., Singh, S.K. and Dash, D. (2012) Effect of Silver Nanoparticles on Growth of Eukaryotic Green Algae. Nano-Micro Letters, 4, 158-165.
[26] Racuciu, M. (2012) Iron Oxide Nanoparticles Coated with β-Cyclodextrin Polluted of Zea mays Plantlets.

comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.