Share This Article:

Schistosoma mansoni: Phytochemical Effect on Aquatic Life Cycle

Abstract Full-Text HTML XML Download Download as PDF (Size:297KB) PP. 127-132
DOI: 10.4236/ojvm.2015.56017    3,936 Downloads   4,361 Views   Citations

ABSTRACT

Background: Two aspects need to be considered for schistosomiasis control: morbidity and transmission. In this context, many soluble substances have been tested and Euphorbia milii latex is one of the most promising Brazilian molluscicides. Phytochemical studies involving simulation of the applicability of this latex on all aquatic forms of the S. mansoni life cycle are rare in the literature. The aim of this work was to evaluate the effect of E. milii latex on S. mansoni in the egg, miracidium and different developmental stages in Biomphalaria glabrata. Methods: The laboratory study was designed to simulate the different forms of exposure of the life cycle stages of S. mansoni to the LC50 of E. milii latex; we tested the exposition from four situations of S. mansoni contact with the latex and observed the exposure on different snails’ infection stage too. All snails were analyzed weekly for cercarial shedding and reproductive biology. Results: The results showed that contact of S. mansoni eggs and miracidia with the LC50 of E. milii negatively influenced the development of the parasite life cycle in the intermediate host, with consequent reduction of cercarial shedding. The exposure of infected snails affected the reproductive biology and cercarial shedding in all intra-mollusk development stages of S. mansoni, but the reduction was greater in the first, fourth, fifth, sixth, seventh and eighth weeks of infection. The LC50 of E. milii latex had toxic action on eggs and miracidia, and the number of cercariae shed by snails during the study period declined by about 80%. Conclusions: We can conclude that the use of natural biodegradable compounds containing low concentrations of substances already characterized as having eco-toxico- logical potential can be an important tool to reduce the transmission of Schistosomiasis.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Augusto, R. C., Friani, G., de Vasconcellos, M. C., de Azevedo Rodrigues, M. de L. and Mello-Silva, C. C. (2015) Schistosoma mansoni: Phytochemical Effect on Aquatic Life Cycle. Open Journal of Veterinary Medicine, 5, 127-132. doi: 10.4236/ojvm.2015.56017.

References

[1] Giovanelli, A., Silva, C.L.P.A.C., Medeiros, L. and Vasconcelos, M.C. (2001) The Molluscicidal activity of the Latex of Euphorbia splendens var. hislopii on Melanoides tuberculata (Thiaridae), a Snail Associated with Habitats of Bio- mphalaria glabrata (Planorbidae). Memórias do Instituto Oswaldo Cruz, 96, 123-125.
http://dx.doi.org/10.1590/S0074-02762001000100014
[2] Mello-Silva, C.C., Vilar, M.M., Vasconcellos, M.C., Pinheiro, J. and Rodrigues, M.L.A. (2006) Physiological Changes in Biomphalaria glabrata Say, 1818 (Pulmonata: Planorbidae) Caused by Sub-Lethal Concentrations of the Latex of Euphorbia splendens var. Hislopii N.E.B (Euphorbiaceae). Memórias do Instituto Oswaldo Cruz, 101, 3-8.
http://dx.doi.org/10.1590/S0074-02762006000100002
[3] Lima, M.G., Augusto, R.C., Vasconcellos, M.C., Mello-Silva, C.C. and Pinheiro, J. (2012) Metabolic Changes in Biomphalaria glabrata Infected with Schistosoma mansoni Exposed to Latex of Euphorbia milii Solution versus Times of Preparation. Journal of Natural Products, 5, 222-232.
[4] Mello-Silva, C.C., Vilar, M.M., Vasconcellos, M.C., Pinheiro, J. and Rodrigues, M.L.A. (2010) Carbohydrate Metabolism Alterations in the Biomphalaria glabrata Infected with Schistosoma mansoni and Exposed to Euphorbia splendens var. hislopii Latex. Memórias do Instituto Oswaldo Cruz, 105, 492-495.
http://dx.doi.org/10.1590/S0074-02762010000400024
[5] Vasconcellos, M.C. and Amorin, A. (2003) Molluscicidal Action of the Latex of Euphorbia splendens var. hislopii N.E.B (“Christ’s Crown”) (Euphorbiaceae) against Lymnaea columella (Say, 1817) (Pulmonata: Lymnaeidae), Intermediate Host of Fasciola hepatica Linnaeus, 1758 (Trematoda: Fasciolidae). 1. Test in Laboratory. Memórias do Instituto Oswaldo Cruz, 98, 557-563.
http://dx.doi.org/10.1590/S0074-02762003000400025
[6] Schall, V.T., Vasconcellos, M.C., Rocha, R.S., Souza, C.P. and Mendes, N.M. (2001) The Control of the Schistosome-Transmitting Snail Biomphalaria glabrata by the Plant Molluscicide Euphorbia splendens var. hislopii (syn milli Des. Moul): A Longitudinal Field Study in an Endemic Area in Brazil. Acta Tropica, 79, 165-170.
http://dx.doi.org/10.1016/S0001-706X(01)00126-7
[7] Thornhill, J., Coelho, P.M., McVeigh, P., Maule, A., Jurberg, A.D. and Kusel, J.R. (2009) Schistosoma mansoni Cercariae Experience Influx of Macromolecules during Skin Penetration. Parasitology, 136, 1257-1267.
http://dx.doi.org/10.1017/S0031182009990692
[8] Mello-Silva, C.C., Vilar, M.M., Bezerra, J.C., Vasconcellos, M.C., Pinheiro, J. and Rodrigues, M.L.A. (2007) Reproductive Activity Alterations on the Biomphalaria glabrata Exposed to Euphorbia splendens var. hislopii Latex. Memórias do Instituto Oswaldo Cruz, 102, 671-674.
http://dx.doi.org/10.1590/S0074-02762007000600002
[9] Mello-Silva, C.C., De Vasconcellos, M.C., Bezerra, J.C.B., Rodrigues, M.L.A. and Pinheiro, J. (2011) The Influence of Exposure to Euphorbia splendens var. hislopii Latex on the Concentrations of Total Proteins and Nitrogen Products in Biomphalaria glabrata Infected with Schistosoma mansoni. Acta Tropica, 117, 101-104.
http://dx.doi.org/10.1016/j.actatropica.2010.10.007
[10] Finney, D.J. (1971) Probit Analysis. Cambridge University Press, Cambridge, 333 p.
[11] Jong-Brink, M., Elsaadany, M.M. and Boer, H.H. (1988) Schistosomin, an Antagonist of Calfluxin. Experimental Parasitology, 65, 109-118.
http://dx.doi.org/10.1016/0014-4894(88)90112-9
[12] Faro, M.J., Perazzini, M., Corrêa, L.R., Mello-Silva, C.C., Pinheiro, J., Mota, E.M., Souza, S., Andrade, Z. and Maldonado Jr., A. (2013) Biological, Biochemical and Histopathological Features Related to Parasitic Castration of Biomphalaria glabrata Infected by Schistosoma mansoni. Experimental Parasitology, 134, 228-234.
http://dx.doi.org/10.1016/j.exppara.2013.03.020
[13] Carvalho, O. dos S., Coelho, P.M.Z. and Lenzi, H.L. (2008) Schistosoma mansoni e esquistossomose: Uma visão multidisciplinar. Editora Fiocruz, Rio de Janeiro, 1123 p.
http://dx.doi.org/10.7476/9788575413708

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.