Effectiveness of Selected Reaction Monitoring for rapid assay of Cypermethrin Residue in Perilla Leaves
Noriyasu Niimura
DOI: 10.4236/ampc.2011.11001   PDF   HTML     3,514 Downloads   7,926 Views   Citations


Many kinds of pesticides have been developed and used to yield a good harvest but the residues in agricul-tural products cause health problems. It is important to keep watch on these residues by using adequate methods of analysis. Pretreatment such as gel permeation chromatography (GPC) or column chromatography is often needed for the quantitative analysis of pesticide in agricultural products by conventional methods such as gas chromatography/low resolving power mass spectrometry (GC/LRMS). However, these pretreat-ments need a lot of work and take time. New methods saving the necessity of these pretreatments have been desired. We have applied selected reaction monitoring (SRM) to quantitatively determine cypermethrin residues in Perilla frutescens samples and compared the results with LRMS and HRMS in SIM mode. A background peak caused by the matrix overlapped the cypermethrin peak in the analysis using LRMS. SRM and HRMS in SIM mode provided chromatograms without matrix interference. The high selectivity of the product ion (m/z 127) produced from precursor ion (m/z 163) isolated the target peaks from the matrix peaks when using SRM. This method eliminates the pretreatment step, thus saving time and simplify ing the ana-lytical process.

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N. Niimura, "Effectiveness of Selected Reaction Monitoring for rapid assay of Cypermethrin Residue in Perilla Leaves," Advances in Materials Physics and Chemistry, Vol. 1 No. 1, 2011, pp. 1-5. doi: 10.4236/ampc.2011.11001.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] European Food Safety Authority, “Conclusion on Pesti-cide Peer Review,” EFSA Scientific Report, Vol. 196, 2008, pp. 1-119196 (2008) 2.
[2] M. Okihashi, H. Obana, S. Hori, T. Nishimune, “Devel-opment of Simultaneous Analysis for Organonitrogen and Pyrethroid Pesticides with GC/MS,” Shokueishi, Vol. 35, No. 3, 1994, 258-261.
[3] Ch. Lentza-Rizos, E. J. Avramides and E. Visi, “Deter-mination of Residues of Endosulfan and Five Pyrethroid Insecticides in Virgin Olive Oil Using Gas Chromatog-raphy with Electron-capture Detection,” Journal of Chromatography A, Vol. 921, No. 2, 2001, pp. 297-304. doi:10.1016/S0021-9673(01)00874-3
[4] Y. Chatani and M. Komatsu, “Studies on Simultaneous Determination of Pesticide Residues in Tabacco Leaf by GC/MS,” Kyouto-Fu Hoken-Kan-kyou-Kenkyujo Nenpou, Vol.45, 2000, pp. 12-15.
[5] J. Cai, B. Z. Liu, X. L. Zhu and Q. D. Su, “Determination of Pyrethroid Residues in Tobacco and Cigarette Smoke by Capillary Gas Chromatography,” Journal of Chroma-tography A, Vol. 964, No. 1-2, 2002, pp. 205-211. doi:10.1016/S0021-9673(02)00586-1
[6] C. Ferrer, M. J. Gomez, J. F. Garcia-Reyes, I. Ferrer, E. M. Thurman and A. R. Fernandes-Alba, “Determina-tion of Pesticide Residues in Olives and Olive Oil by Matrix Solid-Phase Dispersion Followed by Gas Chromatography/Mass Spectrometry and Liquid Chromatography/Tandem Mass Spectrometry,” Journal of Chromatography A, Vol. 1069, No. 2, 2005, pp. 183- 194. doi:10.1016/j.chroma.2005.02.015
[7] I. R. Pizzutti, R. J. J. Vreuls, A. de Kok, R. Roehrs, S. Martel, C. A. Friggi and R. Zanella, “Design of a Com-pressed Air Modulator to Be Used in Comprehensive Multidimensional Gas Chromatography and Its Applica-tion in the Determination of Pesticide Residues in Grapes,” Journal of Chromatography A, Vol. 1216, No. 15, 2009, pp. 3305-3311. doi:10.1016/j.chroma.2009.01.088
[8] A. Di Muccio, D. A. Barbini, T. Generali, P. Pelosi, A. Ausili, F. Vergori and I. Camoni, “Clean-up of Aqueous Acetone Vegetable Extracts by Solid-Matrix Partition for Pyrethroid Residue Determination by Gas Chromatogra-phy—Electron-capture Detection,” Journal of Chroma-tography A, Vol. 765, No. 1, 1997, pp. 39-49.
[9] Y. Nakamura, Y. Tonogai, Y. Tsumura and Y. Ito, “De-termination of pyrethroid residues in vegetables, fruits, grains, beans and green tea leaves: applications to pyre-throid residue monitoring studies,” J. AOAC Interna-tional, Vol. 76, No. 6, 1993, pp. 1348-1361.
[10] K. L. Busch, G. L. Glish and S. A. McLuckey, “Mass Spectrometry/Mass Spectrometry: Techniques and Ap-plications of Tandem Mass Spectrometry,” VCH, New York, 1988.
[11] E. D. Hoffmann and V. Stroobant, “Mass Spectrometry: Principle and Applications,” 3rd Edition, John Wiley & Sons Ltd, Chichester, 2007.

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