Induced Monocytes-Derived HSCs (CD34+) with LPS Accelerated Homing Rat Bone Marrow-Mesenchymal Stem Cell (BM-MSCs, CD105) in Injured Pancreas
Fedik A. Rantam1,2*,   Purwati1,3, Budi Setiawan4, Sony Wibisono3,   Ferdiansyah1,5, Joni Wahyuhadi1,6, Edward Mouli1,5, Dwikora N. Utomo1,5, Heri Suroto1,5, Candra Bumi1
1Stem Cell Laboratory, Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia.
2Virology and Immunology Laboratory, Department of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
3Tropic and Infection Division of Internal Medicine, School of Medicine, Airlangga University, Surabaya, Indonesia.
4Animal Surgery, Animal Hospital, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
5Regenerative Medicine, Department of Orthopedic, Dr. Soetomo Teaching Hospital/School of Medicine, Airlangga University, Surabaya, Indonesia.
6Department of Neurosurgery, Dr. Soetomo Teaching Hospital/School of Medicine, Airlangga University, Surabaya, Indonesia.
DOI: 10.4236/jbise.2015.85031   PDF   HTML   XML   3,357 Downloads   4,426 Views   Citations

Abstract

Investigating the function of combining induced rat monocytes-derived bone marrow-haemopoietic stem cell (rat BM-HSCs) with LPS and rat bone marrow-mesenchymal stem cell (rat BM-MSCs) was to analyze the acceleration of homing process mechanism in injured pancreas. Mononucleated stem cells were isolated from aspirated whole rat BM using ficoll and cultured in α-MEM complete growth medium in 10 cm petridish. After two days, adherent cells after washing twice in petridish were added α-MEM growth medium and then mesenchymal cells were characterized using CD105 marker in third passage and labeled PKH26. Then haemopoietic stem cells (HSCs) were isolated with magnetic beads CD34+ and differentiated in vitro, and then induced monocytes with LPS. Animal experiment used 28 male Wistar rats, and divided them into 4 groups. After transplantation combined, both cells between monocyte derived HSc (mHSCs) and rat BM-MSC were analyzed expression of pair box gen 4 (Pax4), pancreatic and duodenal homeobox (Pdx1), C-peptide using immunohistochemistry, then secretion of insulin and C-peptide analyzed using indirect ELISA. Results showed that the expressions of Pax4, Pdx1, C-peptide found in the surface membrane cell of pancreatic cell, and secreted C-peptide and insulin were shown significant (P < 0.05) in transplanted group 2, 3 and 4, but in group 3 were transplanted with combined cells more dominant than non-combined cells. Conclusions suggested that combining of induced monocytes-derived HSCs and rat BM-MSCs has accelerated homing MSCs into injured pancreatic tissue.

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Rantam, F. , Purwati,  . , Setiawan, B. , Wibisono, S. , Ferdiansyah,  . , Wahyuhadi, J. , Mouli, E. , Utomo, D. , Suroto, H. and Bumi, C. (2015) Induced Monocytes-Derived HSCs (CD34+) with LPS Accelerated Homing Rat Bone Marrow-Mesenchymal Stem Cell (BM-MSCs, CD105) in Injured Pancreas. Journal of Biomedical Science and Engineering, 8, 333-344. doi: 10.4236/jbise.2015.85031.

Conflicts of Interest

The authors declare no conflicts of interest.

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