Plant Regeneration Through Indirect Somatic Embryogenesis in Coelogyne Cristata Orchid

DOI: 10.4236/ajps.2011.22028   PDF   HTML     7,723 Downloads   15,759 Views   Citations


A newly efficient protocol has been established for high frequency somatic embryogenesis through callus culture of Coelogyne cristata. The best frequency of callusing was obtained from leaf segments (3-5 mm) cultured on the MS medium supplemented with 2 mg?L–1 2,4-D and 2 mg?L–1 BA combination. A negative effect of coconut water was ob-served on the callus induction medium. When callus lines no. 4, 6 and 8 induced from leaf segments were sub-cultured separately on ½ MS and MS media containing AC (1-3 g?L–1), formation of somatic embryos was found. However, percentages of embryo formation and the number of embryos per explants were strongly affected by media and callus lines used. The effect of 1/2 MS media is definitely better than MS medium for somatic embryogenesis from the selected lines of leaf derived callus. Among the callus lines, line no. 4 is the best for somatic embryogenesis followed by line no. 6 and 8. The somatic embryos converted into healthy plants with well developed shoots on the same media. The plant-lets were transferred to 1/2 MS medium containing 1 g?L–1 AC for plant regeneration until 8 weeks of culture and successfully acclimatized in the greenhouse.

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A. Naing, J. Chung and K. Lim, "Plant Regeneration Through Indirect Somatic Embryogenesis in Coelogyne Cristata Orchid," American Journal of Plant Sciences, Vol. 2 No. 2, 2011, pp. 262-267. doi: 10.4236/ajps.2011.22028.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] G. M. Andrade and S. A. Merkle, “Enhancement of American chestnut somatic seedling production,” Plant Cell Rep, Vol. 24, No. 6, August 2005, pp. 326-334A.
[2] A. Begum, M. Tamaki and S. Kako, “Formation of Protocorm-like-bodies (PLB) and shoot development through in vitro culture of outer tissue of Cymbidium PLB,” J. Japan.Soc. Hort. Sci, Vol. 63, No. 3, 1994, pp.663-673
[3] J. T. Chen, C. Chang and W. C. Chang, “Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey and subsequent plant regeneration,” Plant Cell Report,. Vol. 19, No. 2, December 1999, pp. 143-149.
[4] J. T. Chen and W. C. Chang, “Effects of auxins and cytokinins on direct somatic embryogenesis on leaf explants of Oncidium ‘Gower Ramsey,” Plant Growth Regulation, Vol. 34, No. 2, June 2001, pp. 229-232.
[5] H. H. Chung, J. T. Cheng and W. C. Chang, “Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium,” Biologia Plantarum Vol. 51, No. 2, June 2007, pp. 346-350.
[6] S. Fiore, F. D. Pasquale, F. Carimi and M. Sajeva, “Effect of 2,4-D and 4-CPPU on somatic embryogenesis from stigma and style transverse thin cell layers of Citrus,” Plant Cell Tiss Organ Cult, Vol. 68, No. 1, January 2002, pp. 57-63
[7] G. Gambino, P. Ruffa, R, Vallania and I. Gribaudo, “Somatic embryogenesis from whole flowers, anthers and ovaries of grapevine (Vitis spp),” Plant Cell Tiss Organ Cult, Vol. 90, No. 1, January 2007, pp. 79-83.
[8] L. V. T. Huana, T. Takamura and M. Tanaka, “Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid,” Plant Science, Vol. 166, No. 6, June 2004, pp. 1443-1449.
[9] Y. Ishii, T. Takamura, M. Goi and M. Tanaka, “Callus induction and somatic embryogenesis of Phalaenopsis,” Plant Cell Reports, Vol. 17, No. 6-7, April 1998, pp. 446-450.
[10] G. B. Kerbauy, “Plant regeneration of Oncidium varicosum (Orchidaceae) by means of root tip culture,” Plant Cell Reports, Vol. 3, No. 1, February 1984, pp. 27-29.
[11] H. L. Kuo, J. T. Chen and W. C. Chang, “Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis “Little Steve”,” In Vitro Cell. Dev. Biol.—Plan,. Vol. 41, No. 4, July 2005, pp. 453-456.
[12] N. T. Lang and N. T. Hang, “Using biotechnological approaches for Vanda orchid Improvement,” Omonrice, Vol. 14, 2006, pp.140-143.
[13] Y. Lee and N. Lee, “Plant regeneration from protocorm-derived callus of Cypripedium formosanmun,” In Vitro Cell. Dev. Biol.—Plan, Vol. 39, No. 5, September 2003, pp. 475-479.
[14] Y. H. Lin, C. Chang and W. C Chang, “Plant regeneration from callus culture of a Paphiopedilum hybrid,” Plant Cell Tiss Organ Cult, Vol. 62, No. 1, July 2000, pp. 21-25.
[15] S. Y. Motoike, E. S. Saraiva, M. C. Ventrella, C. V. Silva and L. C. C Saloma?o, “Somatic embryogenesis of Myrciaria aureana (Brazilian grape tree),” Plant Cell Tiss Organ Cult, Vol. 89, No. 1, April 2007, pp.75-81.
[16] J. Roy and N. Banerjee, “Induction of callus and plant regeneration from shoot tip explants of Dendrobium fimbriatum Lindl. var. oculatum Hk.f,” Scientia Horticulturae, Vol. 97, No. 3, February 2003, pp. 333–340.
[17] J. F. Wu, J. T. Chen and W. C. Chang, “Effects of auxins and cytokinins on embryo formation from root-derived callus of Oncidium ‘Gower Ramsey,” Plant Cell, Tiss Organ Cult, Vol. 77, No. 1, April 2004, pp. 107-109.

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