The Effects of Multilocus Heterozygosity on the Longevity of Seedlings Established on Fallen Logs in Picea jezoensis and Abies sachalinensis


Seedling establishment on fallen logs is a major regeneration system for tree species in boreal forests. Seedling survival on fallen logs is affected not only by the microsite environment but also by the genetic factors of individuals. To quantify the genetic effects on seedling longevity, we identified seedlings using a number tag system and collected needles of Picea jezoensis and Abies sachalinensis established on fallen logs in spring 2006. Survival or death of each seedling was investigated during 2006-2012. We genotyped seedlings with microsatellite markers and calculated individual-based multilocus heterozygosity (MLH) for each seedling. A Cox proportional hazards model was applied to evaluate the effects of MLH on seedling longevity of the two species considering the fallen log conditions. The model indicated that MLH positively affected seedling longevity in P. jezoensis, whereas the effects of MLH were not significant in A. sachalinensis. Here, we discuss differences in the effects of MLH on seedling longevity between the two species, considering species characteristics and MLH frequency distribution.

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Okada, M. , Kitamura, K. , Lian, C. and Goto, S. (2015) The Effects of Multilocus Heterozygosity on the Longevity of Seedlings Established on Fallen Logs in Picea jezoensis and Abies sachalinensis. Open Journal of Forestry, 5, 422-430. doi: 10.4236/ojf.2015.54036.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Asakawa, S., Katsuta, M., & Yokoyama, T. (1981). Seeds of Woody Plants in Japan: Gymnospermae (pp. 150). Tokyo: Forest Tree Breeding Association. (In Japanese)
[2] Besnard, G., Acheré, V., Besnard, G., Faivre, V., Rampant, P., Favre, J. M., & Jendroz, S. (2003). A Set of Cross-Species Amplifying Microsatellite Markers Developed from DNA Sequence Databanks in Picea (Pinaceae). Molecular Ecology, 3, 380-383.
[3] Chapman, J. R., Nakagawa, S., Coltman, D. W., Slate, J., & Sheldon, B. C. (2009). A Quantitative Review of Heterozygosity-Fitness Correlations in Animal Populations. Molecular Ecology, 18, 2746-2765.
[4] Collevatti, R. G., & Hay, D. (2011). Kin Structure and Genotype-Dependent Mortality: A Study Using the Neotropical Tree Caryocar brasiliense. Journal of Ecology, 99, 757-763.
[5] Forstmeier, W., Schielzeth, H., Mueller, J. C., Ellegren, H., & Kempenaers, B. (2012). Heterozygosity-Fitness Correlations in Zebra Finches: Microsatellite Markers Can Be Better than Their Reputation. Molecular Ecology, 21, 3237-3249.
[6] Hansson, B., Bensch, S., Hasselquist, D., & Akesson, M. (1996). Microsatellite Diversity Predicts Recruitment of Sibling Great Reed Warblers. Proceedings of the Royal Society of London Series B-Biological Sciences, 268, 1287-1291.
[7] Harmon, M. E., & Franklin, J. F. (1989). Tree Seedlings on Logs in Picae-Tsuga Forests of Oregon and Washington. Ecology, 70, 48-59.
[8] Hirao, A. S. (2010). Kinship between Parents Reduces Offspring Fitness in a Natural Population of Rhododendron brachycarpum. Annals of Botany, 105, 637-646.
[9] Hoffman, J. I., Simpson, F., David, P., Rijks, J. M., Kuiken, T., Thorne, M. A., Lacy, R. C., & Dasmahapatra, K. K. (2014). High-Throughput Sequencing Reveals Inbreeding Depression in a Natural Population. Proceedings of the National Academy of Sciences, 111, 3775-3780.
[10] Hufford, K., & Hamrick, J. (2003). Viability Selection at Three Early Life Stages of the Tropical Tree, Platypodium elegans (Fabaceae, Papilionoideae). Evolution, 57, 518-526.
[11] Husband, B. C., & Schemske, D. W. (1996). Evolution of the Magnitude and Timing of Inbreeding Depression in Plants. Evolution, 50, 54-70.
[12] Iijima, H., Shibuya, M., & Saito, H. (2007). Effects of Surface and Light Conditions of Fallen Logs on the Emergence and Survival of Coniferous Seedlings and Saplings. Journal of Forest Research, 12, 262-269.
[13] Iijima, H., & Shibuya, M. (2010). Evaluation of Suitable Conditions for Natural Regeneration of Picea jezoensis on Fallen Logs. Journal of Forest Research, 15, 46-54.
[14] Isagi, Y., Saito, D., Kawaguchi, H., Tateno, R., & Watanabe, S. (2007). Effective Pollen Dispersal Is Enhanced by the Genetic Structure of an Aesculus turbinata Population. Journal of Ecology, 95, 983-990.
[15] Kadomatsu, M., Kudo, H., Noda, M., Natsume, S., & Kanno, T. (1997). Height Growth of Selfed-Pollinated Abies sachalinensis Seedlings over a Fourteen-Year Period. Journal of Japanese Forest Society, 79, 119-122. (In Japanese with English Summary)
[16] Karkkainen, K., Koski, V., & Savolainen, O. (1996). Geographical Variation in the Inbreeding Depression of Scots Pine. Evolution, 50, 111-119.
[17] Keller, L. F., & Waller, D. (1996). Inbreeding Effects in Wild Population. Trends in Ecology and Evolution, 17, 230-241.
[18] Lian, C., Goto, S., Kubo, T., Takahashi, Y., Nakagawa, M., & Hogetsu, T. (2008). Nuclear and Chloroplast Microsatellite Analysis of Abies sachalinensis Regeneration on Fallen Logs in a Subboreal Forest in Hokkaido, Japan. Molecular Ecology, 17, 2948-2962.
[19] Naito, Y., Konuma, A., Iwata, H., Suyama, Y., Seiwa, K., Okuda, T., Lee, S. L., Muhammand, N., & Tsumura, Y. (2005). Selfing and Inbreeding Depression in Seeds and Seedlings of Neobalanocarpus heimii (Dipterocarpaceae). Journal of Plant Research, 118, 423-430.
[20] Narukawa, Y., Iida, S., Tanouchi, H., Abe, S., & Yamamoto, S. I. (2003). State of Fallen Logs and the Occurrence of Conifer Seedlings and Saplings in Boreal and Subalpine Old-Growth Forests in Japan. Ecological Research, 18, 267-277.
[21] O’Hanlon-Manners, D. L., & Kotanen, P. M. (2004). Logs as refuges from fungal pathogens for seeds of eastern hemlock (Tsuga candadensis). Ecology, 85, 284-289.
[22] Pfeiffer, A., Olivieri, A. M., & Morgante, M. (1997). Identification and Characterization of Microsatellites in Norway Spruce (Picea abies K.). Genome, 40, 411-419.
[23] R Development Core Team (2012). R: A Language and Environment for Statistical Computing. Vienna: R Foundation for Statistical Computing
[24] Scotti, I., Magni, F., Paglia, G., & Morgante, M. (2002). Trinucleotide Microsatellites in Norway Spruce (Picea abies): Their Features and the Development of Molecular Markers. Theoretical and Applied Genetics, 106, 40-50.
[25] Takahashi, M., Sakai, Y., Ootomo, R., & Shiozaki, M. (2000). Establishment of Tree Seedlings and Water-Soluble Nutrients in Coarse Woody Debris in an Old-Growth Picea-Abies Forest in Hokkaido, Northern Japan. Canadian Journal of Forest Research, 30, 1148-1155.
[26] Tomita, M., Suyama, Y., & Saito, H. (2008). Effect of Local Stand Density on Reproductive Processes of the Sub-Boreal Conifer Picea jezoensis Carr. (Pinaceae). Forest Ecology and Management, 256, 1350-1355.
[27] Ubukata, M., Koono, K. & Itahana, N. (2000). Estimation of Genetic Parameters on Height Growth Using Artificial Mating Families between Plus Trees of Abies sachalinensis. Bulletin of Forest Tree Breeding Center, 17, 135-151. (In Japanese with English Summary)

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