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RimJ-Catalyzed Sequence-Specific Protein N-Terminal Acetylation in Escherichia coli

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DOI: 10.4236/abb.2015.63018    4,287 Downloads   4,703 Views  

ABSTRACT

In order to establish the sequence dependence of RimJ-mediated protein N-terminal acetylation in E. coli, the Z-domain variants differing by the second or third amino acid residue were expressed and analyzed by mass spectrometry. Only subsequent to the initiating methionine residue cleavage, the RimJ-catalyzed N-terminal acetylation mainly occurred at the N-terminal serine and threonine residues and was significantly enhanced by hydrophobic or negatively charged residues in the penultimate position.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Perez, L. and Ryu, Y. (2015) RimJ-Catalyzed Sequence-Specific Protein N-Terminal Acetylation in Escherichia coli. Advances in Bioscience and Biotechnology, 6, 182-193. doi: 10.4236/abb.2015.63018.

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