Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker
Letícia Sayuri Murate1, Admilton Gonçalves de Oliveira1, Allan Yukio Higashi1, André Riedi Barazetti1, Ane Stefano Simionato1, Caroline Santos da Silva1, Glenda Cavalari Simões1, Igor Matheus Oliveira dos Santos1, Marlon Renan Ferreira1, Martha Viviana Torres Cely1, Miguel Octavio Pérez Navarro1, Vanessa Fogaça de Freitas1, Marco Antonio Nogueira2, João Carlos Palazzo de Mello3, Rui Pereira Leite Jr.4, Galdino Andrade1*
1Department of Microbiology, Londrina State University, Londrina, Brazil.
2Embrapa Soja, Londrina, Brazil.
3Department of Pharmacy and Pharmacology, Maringá State University, Maringá, Brazil.
4Plant Protection, Agronomic Institute of Paraná, IAPAR, Londrina, Brazil.
DOI: 10.4236/as.2015.63030   PDF   HTML   XML   5,329 Downloads   6,571 Views   Citations


This study investigated the protective effects of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was cultured in liquid medium and the supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was concentrated, filtrated, lyophilized and fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions were obtained. All fractions’ activity against Xac 306 by agar well diffusion assay and minimum inhibitory concentration but in different concentrationswere tested. Cytotoxicity effects were observed in all fractions in 50 μg·mL-1 concentration. The comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no genotoxic effects at tested concentrations. In plants only VLC3 showed significant results (p<0.05), reducing the incidence of citrus canker lesions.

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Murate, L. , Oliveira, A. , Higashi, A. , Barazetti, A. , Simionato, A. , Silva, C. , Simões, G. , Santos, I. , Ferreira, M. , Cely, M. , Navarro, M. , Freitas, V. , Nogueira, M. , Mello, J. , Leite Jr., R. and Andrade, G. (2015) Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker. Agricultural Sciences, 6, 295-303. doi: 10.4236/as.2015.63030.

Conflicts of Interest

The authors declare no conflicts of interest.


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