Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

Letícia Sayuri Murate¹, Admilton Gonçalves de Oliveira¹, Allan Yukio Higashi¹, André Riedi Barazetti¹, Ane Stefano Simionato¹, Caroline Santos da Silva¹, Glenda Cavalari Simões¹, Igor Matheus Oliveira dos Santos¹, Marlon Renan Ferreira¹, Martha Viviana Torres Cely¹, Miguel Octavio Pérez Navarro¹, Vanessa Fogaça de Freitas¹, Marco Antonio Nogueira², João Carlos Palazzo de Mello³, Rui Pereira Leite Jr.⁴, Galdino Andrade^1*

¹Department of Microbiology, Londrina State University, Londrina, Brazil.
²Embrapa Soja, Londrina, Brazil.
³Department of Pharmacy and Pharmacology, Maringá State University, Maringá, Brazil.
⁴Plant Protection, Agronomic Institute of Paraná, IAPAR, Londrina, Brazil.

DOI: 10.4236/as.2015.63030                

Abstract

This study investigated the protective effects of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was cultured in liquid medium and the supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was concentrated, filtrated, lyophilized and fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions were obtained. All fractions’ activity against Xac 306 by agar well diffusion assay and minimum inhibitory concentration but in different concentrationswere tested. Cytotoxicity effects were observed in all fractions in 50 μg·mL^-1 concentration. The comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no genotoxic effects at tested concentrations. In plants only VLC3 showed significant results (p<0.05), reducing the incidence of citrus canker lesions.

Keywords

X. axonopodis pv. citri, Citrus sinensis, Antibiosis, Biological Control, Natural Antibiotic

Conflicts of Interest

The authors declare no conflicts of interest.

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