Survival Rate and Biochemical Parameters in Mugil cephalus (Linnaeus, 1758) Larvae Fed Garlic (Allium sativum L.) Extract

DOI: 10.4236/ajmb.2015.51002   PDF   HTML   XML   4,617 Downloads   5,551 Views   Citations


This study was carried out to investigate the effect of different concentrations of garlic (Allium sativum L.) extract in fish diet on survival rate, digestive enzymes and some biochemical parameters of Mugil cephalus larvae. Three hundred and sixty fish with mean weight 0.45 g were randomly divided into equal four groups; each one contained 3 replicates. Fish was fed with diets containing 0% (control), 0.5%, 1% and 3% concentrations of garlic extract (GE) in diet. 30 days after feeding, survival rate, different biochemical (larvae extract total protein, albumin, globulin, glucose, cholesterol and triglyceride) parameters and digestive enzyme activity (lipase, protease and amylase) were evaluated. There was a significant increase in survival rate of all groups fed with GE as compared to the control diet (P < 0.05). The administration of A. sativum in all levels significantly decreased (P < 0.05) the content of cholesterol, triglyceride and glucose in larvae extract. Also total protein, albumin and globulin levels had significantly increased in all groups fed GE (P < 0.05). The highest total protein (2.13 ± 0.12 g/dL), albumin (0.37 ± 0 g/dL), globulin (1.76 ± 0.12 g/dL), amylase (9.25 ± 0.14 U/mg protein), protease (4.20 ± 0.08 U/mg protein) and lipase (2.62 ± 0.14 U/mg protein) and the lowest serum triglyceride (22.78 ± 0.20 mg/dL), glucose (8.76 ± 0.09 mg/dL), cholesterol (3.69 ± 0.07 mg/dL) levels were observed in fish fed 3% GE in diet. Garlic inclusion in fish diet at 3% concentration is therefore beneficial for use in aquaculture to improve the general health and digestive enzyme activity of M. cephalus larvae.

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Fereidouni, M. , Akbary, P. and Soltanian, S. (2015) Survival Rate and Biochemical Parameters in Mugil cephalus (Linnaeus, 1758) Larvae Fed Garlic (Allium sativum L.) Extract. American Journal of Molecular Biology, 5, 7-15. doi: 10.4236/ajmb.2015.51002.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Yelghi, S., Shirangi, S.A., Ghorbani, R. and Khoshbavar Rostami, H.A. (2012) Annual Cycle of Ovarian Development and Sex Hormones of Grey Mullet (Mugil cephalus) in Captivity. Iranian Journal of Fisheries Sciences, 11, 693-703.
[2] Akbary, P., Hosseini, S.A. and Imanpoor, M.R. (2011) Enrichment of Artemia Nauplii with Essential Fatty Acids and Vitamin C: Effect on Rainbow Trout (Oncorhynchus mykiss) Larvae Performance. Iranian Journal of Fisheries Sciences, 10, 557-569.
[3] Girri, S.S., Sahoo, S.K., Shu, B.B., Sahu, A.H., Mohanty, S.N., Mohanty, P.K. and Ayyappan, S. (2002) Larval Survival and Growth in Wallago attu (Bloch and Schneider): Effects of Light Photoperiod and Feeding Regims. Aquaculture, 213, 157-161.
[4] Citarasu, T., Sekar, R.R., Babu, M.M. and Marian, M.P. (2002) Developing Artemia Enriched Herbal Diet for Producing Quality Larvae in Penaeus monodon. Asian Fisheries Science, 15, 21-32.
[5] Sivaram, V., Babu, M.M., Citarasu, T., Immanuel, G., Murugadass, S. and Marian, M.P. (2004) Growth and Immune Response of Juvenile Greasy Groupers (Epinephelus tauvina) Fed with Herbal Antibacterial Active Principle Supplemented Diets against Vibrio harveyi Infections. Aquaculture, 237, 9-20.
[6] Shin, S.H. and Kim, M.K. (2004) Effect of Dired Powders or Ethanol Extracts of Garlic Flesh and Peel on Lipid Metabolism and Antithrombogenic Capacity in 16-Month-Old Rats. Hanguk Yongyang Hakhoechi, 37, 515-524.
[7] Ebrahim Dorche, I., Tangestani, R., Alizadeh Dvghyklayy, E. and Zare, P. (2013) Effect of Different Levels of Garlic Essential Oil on Growth, Feed and Carcass Composition of Beluga (Huso huso) Rearing Young. Journal of Marine Science and Technology, 11, 1-12.
[8] Sahu, S., Das, B.K., Mishra, B.K., Pradhan, J. and Sarangi, N. (2007) Effect of Allium sativum on the Immunity and Survival of Labeo rohita Infected with Aeromonas hydrophila. Journal of Applied Ichthyology, 23, 80-86.
[9] Shalaby, A.M., Khattab, Y.M. and Abdel Rahman, A.M. (2006) Effects of Garlic (Allium sativum) and Chloramphenicol on Growth Performance, Physiological Parameters and Survival of Nile Tilapia (Oreochromis niloticus). Journal of Venomous Animals and Toxins Including Tropical Diseases, 12, 172-201.
[10] Nwabueze, A.A. (2012) The Effect of Garlic (Allium sativum) on Growth and Haematological Parameters of Clarias gariepinus (Burchell, 1822). Sustainable Agriculture Research, 1, 222-228.
[11] Lee, D.H., Ra, C.S., Song, Y.H., Sung, K.I. and Kim, J.D. (2012) Effects of Dietary Garlic Extract on Growth, Feed Utilization and Whole Body Composition of Juvenile Sterlet Sturgeon (Acipenser ruthenus). Asian-Australasian Journal of Animal Sciences, 25, 577-583.
[12] Khalil, R.H., Nadia, B.M. and Soliman, M.K. (2001) Effects of Biogen and Levamisol HCl on the Immune Response of Cultured Oreochromis niloticus to Aeromonas hydrophila Vaccine. Beni-Suef Veterinary Medicine Journal Egypt, 11, 381-392.
[13] Lee, D.H., Lim, S.R., Ra, C.S. and Kim, J.D. (2012) Effects of Dietary Garlic Extracts on Whole Body Amino Acid and Fatty Acid Composition, Muscle Free Amino Acid Profiles and Blood Plasma Changes in Juvenile Sterlet Sturgeon, Acipenser ruthenus. Asian-Australasian Journal of Animal Sciences, 25, 1419-1429.
[14] Vazquez-Prieto, M.A., Lanzi, C.R., Lembo, C., Galmarini, C.R. and Miatello, R.M. (2011) Garlic and Onion Attenuates Vascular Inflammation and Oxidative Stress in Fructose-Fed Rats. Journal of Nutrition and Metabolism, 2011, Article ID: 475216.
[15] Ademiluyi, A.O., Oboh, G., Owoloye, T.R. and Agbebi, O.J. (2013) Modulatory Effects of Dietary Inclusion of Garlic (Allium sativum) on Gentamycin-Induced Hepatotoxicity and Oxidative Stress in Rats. Asian Pacific Journal of Tropical Biomedicine, 3, 470-475.
[16] Yousefian, M., Ghanei, M., Pourgolam, R. and Rostami, H.K. (2009) Gonad Development and Hormonal Induction in Artificial Propagation of Grey Mullet, Mugil cephalus L. Research Journal of Fisheries and Hydrobiology, 4, 35-40.
[17] Fawcette, J.K. and Scott, J.E. (1960) Practical Clinical Biochemistry. 4th Edition, Arnold Harold Varley, 119-122.
[18] Henry, R.J. (1968) Clincal Chemistry, Principles and Techniques. Harper and Row, New York, 664-666.
[19] Bernfeld, P. (1951) Amylase a and b in Methods in Enzymes. Academic Press, New York, 144-147.
[20] Worthington, C.C. (1991) Worthington Enzyme Manual Related Biochemical. 3rd Edition, Freehold, 212-215.
[21] Javadzadeh, M., Salarzadeh, A.R., Yahyavi, M., Hafezieh, M. and Darvishpour, H. (2012) Effect of Garlic Extract on Growth and Survival Rate in Litopenaeus vannami Post Larval. Iranian Scientific Fisheries Journal, 21, 39-46.
[22] Hussein, S.A. (1996) Electrophoretic Pattern of Serum Protein and Immunoglobulin Level in Chickens in Relation of Age. Benha Veterinary Medicine Journal, 7, 95-107.
[23] Wu, G., Yuan, C., Shen, M., Tang, J., Gong, Y., Li, D., Sun, F., Huang, C. and Han, X. (2007) Immunological and Biochemical Parameters in Carp (Cyprinus carpio) after Qompsell Feed Ingredients for Long-Term Administration. Aquaculture Research, 38, 246-255.
[24] Ravi, S.P. and Jitender, K.N. (2005) Fish Biology and Ecology Theory: Circulatory System of Fish. University College of Sciences, Osmania University, Hyderabad, 347.
[25] Kumar, G.R. and Reddy, K.P. (1999) Reduced Nociceptive Responses in Mice with Alloxan Induced Hyperglycemia after Garlic (Allium sativum Linn.) Treatment. Indian Journal of Experimental Biology, 37, 662-666.
[26] Thomson, M. and Alim, M. (2003) Garlic (Allium sativum): A Review of Its Potential Use as an Anti-Cancer Agent. Current Cancer Drug Targets, 3, 67-81.
[27] Sheela, C.G. and Augusti, K.T. (1992) Antidiabetic Effects of S-Allyl Cysteine Sulphoxide Isolated from Garlic, Allium sativum Linn. Indian Journal of Experimental Biology, 30, 523-526.
[28] Adler, A.J. and Holub, B.J. (1997) Effect of Garlic and Fish-Oil Supplementation on Serum Lipid and Lipoprotein Concentrations in Hypercholesterolemic Men. The American Journal of Clinical Nutrition, 65, 445-450.
[29] Warshafsky, S., Kamer, R.S. and Sivak, S. (1993) Effect of Garlic on Total Serum Cholesterol: A Meta-Analysis. Annals of Internal Medicine, 119, 599-605.
[30] Venkatramalingam, K., Godvin Christopher, J. and Citarasu, T. (2007) Zingiber officinalis an Herbal Appetizer in the Tiger Shrimp Penaeus monodon (Fabricius) Larviculture. Aquaculture Nutrition, 13, 439-443.
[31] Citarasu, T. (2010) Herbal Biomedicines: A New Opportunity for Aquaculture Industry. Aquaculture International, 18, 403-414.
[32] Platel, K. and Srinivasan, K. (2004) Digestive Stimulant Action of Spices: A Myth or Reality? Indian Journal of Medical Research, 119, 167-179.
[33] Mikaili, P., Maadirad, S., Moloudizargari, M., Aghajanshakeri, S. and Sarahroodi, S. (2013) Therapeutic Uses and Pharmacological Properties of Garlic, Shallot, and Their Biologically Active Compounds. Iranian Journal of Basic Medical Sciences, 16, 1031-1048.

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