Influence of Heavy Metals on Seed Germination and Early Seedling Growth in Crambe abyssinica, a Potential Industrial Oil Crop for Phytoremediation

DOI: 10.4236/ajps.2015.61017   PDF   HTML   XML   4,119 Downloads   4,978 Views   Citations

Abstract

The influence of essential (Cu, Ni and Zn) and non-essential heavy metals (Hg, Cr, Pb and Cd) on seed germination and early seedling growth in industrial oil crop Crambe abyssinica was evaluated under laboratory conditions. Our results indicated that among the 7 heavy metals tested only Cu and Hg significantly (P < 0.01) decreased Crambe seed germination in a dose-dependent manner at higher concentrations while certain Cr concentrations significantly increased the seed germination (P < 0.05). All the 7 heavy metals decreased significantly relative root length, shoot length and fresh seedling weight in a dose-dependent manner (P < 0.01). The heavy metals except Ni decreased relative root length first, then shoot length or fresh seedling weight, and finally seed germination. Ni seemed to influence the relative fresh seedling weight first, then shoot length, root length and finally seed germination at lower concentrations, but the decrease in relative root length became faster when the Ni concentrations were increased. Our results indicated that Crambe is tolerant or moderately tolerant to the heavy metals tested except Ni and can be improved for phytoremediation of soils contaminated by heavy metals.

Share and Cite:

Hu, J. , Deng, Z. , Wang, B. , Zhi, Y. , Pei, B. , Zhang, G. , Luo, M. , Huang, B. , Wu, W. and Huang, B. (2015) Influence of Heavy Metals on Seed Germination and Early Seedling Growth in Crambe abyssinica, a Potential Industrial Oil Crop for Phytoremediation. American Journal of Plant Sciences, 6, 150-156. doi: 10.4236/ajps.2015.61017.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Munzuroglu, O. and Geckil, H. (2002) Effects of Metals on Seed Germination, Root Elongation, and Coleoptile and Hypocotyls Growth in Triticum aestivum and Cucumis sativus. Archives of Environment Contamination and Toxicology, 43, 203-213. http://dx.doi.org/10.1007/s00244-002-1116-4
[2] Kramer, U. (2005) Phytoremediation: Novel Approaches to Cleaning up Polluted Soils. Current Opinion in Biotechnology, 16, 133-141. http://dx.doi.org/10.1016/j.copbio.2005.02.006
[3] Cunningham, S.D. and Ow, D.W. (1996) Promises and Prospects of Phytoremediation. Plant Physiology, 110, 715-719.
[4] Salt, D.E., Smith, R.D. and Raskin, I. (1998) Phytoremediation. Annual Review of Plant Physiology and Plant Molecular Biology, 49, 643-668. http://dx.doi.org/10.1146/annurev.arplant.49.1.643
[5] Mitch, M.L. (2002) Phytoextration of Toxic Metals: A Review of Biological Mechanism. Journal of Environment Quality, 31, 109-120. http://dx.doi.org/10.2134/jeq2002.0109
[6] McGrath, S.P. and Zhao, F.J. (2003) Phytoextraction of Metals and Metalloids from Contaminated Soils. Current Opinion in Biotechnology, 14, 277-282. http://dx.doi.org/10.1016/S0958-1669(03)00060-0
[7] Glick, B.R. (2003) Phytoremediation: Synergistic Use of Plants and Bacteria to Clean up the Environment. Biotechnology Advances, 21, 383-393. http://dx.doi.org/10.1016/S0734-9750(03)00055-7
[8] Pulford, I.D. and Watson, C. (2003) Phytoremediation of Heavy Metal-Contaminated Land by Tree—A View. Environment International, 29, 529-540. http://dx.doi.org/10.1016/S0160-4120(02)00152-6
[9] Baker, A.J.M., McGrath, S.P., Reeves, R.D. and Smith, J.A.C. (2000) Metal Hyperaccumulator Plants: A Review of the Ecology and Physiology of a Biochemical Resource for Phytoremediation of Metal-Polluted Soils. In: Terry, N. and Bauelos, G., Eds., Phytoremediation of Contaminated Soil and Water, Lewis Publishers, Florida, 85-107.
[10] Liu, Y., G., Zhang, Y.X. and Chai, T.Y. (2011) Phytochelatin Synthase of Thlaspi caerulescens Enhanced Tolerance and Accumulation of Heavy Metals When Expressed in Yeast and Tobacco. Plant Cell Reports, 30, 1067-1076. http://dx.doi.org/10.1007/s00299-011-1013-2
[11] Yang, X.E., Long, X.X., Ye, H.B., He, Z.L., Stoffella, P.J. and Calvert, D.V. (2004) Cadmium Tolerance and Hyperaccumulation in a New Zn-Hyperaccumulating Plant Species (Sedum alfredii Hance). Plant and Soil, 259, 181-189. http://dx.doi.org/10.1023/B:PLSO.0000020956.24027.f2
[12] Li, T.Q., Yang, X.E., Jin, X.F., He, Z.L., Stoffella, P.J. and Hu, Q.H. (2005) Root Responses and Metal Accumulation in Two Contrasting Ecotypes of Sedum alfredii Hance under Lead and Zinc Toxic Stress. Journal of Environment Science and Health, Part A. Toxic and Hazardous Substance Environmental Engineering, 40, 1081-1096.
[13] Ma, L.Q., Komar, K.M., Tu, C., Zhang, W.H., Cai, Y. and Kennelley, E.D. (2001) A Fern That Hyperaccumulates Arsenic: A Hardy, Versatile, Fast-Growing Plant Helps to Remove Arsenic from Contaminated Soils. Nature, 479, 579. http://dx.doi.org/10.1038/35054664
[14] Mathews, S., Rathinasabapathi, B. and Ma, L.Q. (2011) Uptake and Translocation of Arsenite by Pteris vittala L.: Effects of Glycerol, Antimonite and Silver. Environmental Pollution, 159, 3490-3495. http://dx.doi.org/10.1016/j.envpol.2011.08.027
[15] Yang, C.J., Zhou, Q.X., Wei, S.H., Hu, Y.H. and Bao, Y.Y. (2011) Chemical-Assisted Phytoremediation of Cd-PAHs Contaminated Soils Using Solanum nigrum L. International Journal of Phytoremediation, 13, 818-833. http://dx.doi.org/10.1080/15226514.2010.532179
[16] Kupper, H., Lombi, E., Zhao, F.J. and McGrath, S.P. (2000) Cellular Compartmentation of Cadmium and Zinc in Relation to Other Elements in the Hyperaccumulator Arabidopsis halleri. Planta, 212, 75-84. http://dx.doi.org/10.1007/s004250000366
[17] Morishirta, T. and Boratynski, K. (1992) Accumulation of Cd and Other Metals in Organs of Plants Growing around Metal Smelters in Japan. Soil Science and Plant Nutrition, 38, 781-785.
http://dx.doi.org/10.1080/00380768.1992.10416712
[18] Carlson, K.D., Gardner, J.C., Anderson, V.L. and Hanzel, J.J. (1996)Crambe: New Crop Success. In: Janick, J., Ed., Progress in New Crops, ASHS Press, Alexandria, 306-322.
[19] Wang, Y.P., Tang, J.S., Chu, C.Q. and Tian, J. (2000) A Preliminary Study on the Introduction and Cultivation of Crambe abyssinica in China, an Oil Plant for Industrial Uses. Industrial Crop Production, 12, 47-52. http://dx.doi.org/10.1016/S0926-6690(99)00066-7
[20] Huang, B., Yang, Y., Luo, T., Wu, S., Du, X., Cai, D., Loo, E.N.V. and Huang, B. (2013) Correlation, Regression and Path Analyses of Seed Yield Components in Crambe abyssinica, a Promising Industrial Oil Crop. American Journal of Plant Sciences, 4, 42-47. http://dx.doi.org/10.4236/ajps.2013.41007
[21] Li, X., van Loo, E.N., Gruber, J., Fan, J., Guan, R., Frentzen, M., Stymne, S. and Zhu, L.H. (2012) Development of Ultra-High Erucic Acid Oil in the Industrial Oil Crop Crambe abyssinica. Plant Biotechnology Journal, 10, 862-870. http://dx.doi.org/10.1111/j.1467-7652.2012.00709.x
[22] European Union Strategic Research Agenda (2005) Strategic Research Agenda, Part II: Plant for the Future. Stakeholder Proposal for a Strategic Research Agenda 2005 Including Draft Action Plan 2010.
[23] Artus, N.N. (2006) Arsenic and Cadmium Phytoextraction Potential of Crambe Compared with Indian Mustard. Journal of Plant Nutrition, 29, 667-679. http://dx.doi.org/10.1080/01904160600564444
[24] Paulose, B., Kandasamy, S. and Dhankher, O.P. (2010) Expression Profiling of Crambe abyssinica under Arsenate Stress Identifies Genes and Gene Networks Involved in Arsenic Metabolism and Detoxification. BMC Plant Biology, 10, 108. http://dx.doi.org/10.1186/1471-2229-10-108
[25] Mahmood, T., Islam, K.R. and Muhammad, S. (2007) Toxic Effects of Heavy Metals on Early Growth and Tolerance of Cereal Crops. Pakistan Journal of Botany, 39, 451-462.
[26] Aydinalp, C. and Marinova, S. (2009) The Effects of Heavy Metals on Seed Germination and Plant Growth on Alfalfa Plant (Medicago sativa). Bulgarian Journal of Agricultural Science, 15, 347-350.
[27] Taylor, G.J. and Foy, C.D. (1985) Differential Uptake and Toxicity of Ionic and Chelated Copper in Triticum aestivum. Canadian Journal of Botany, 63, 1271-1275. http://dx.doi.org/10.1139/b85-176
[28] Wheeler, D.M., Power, I.L. and Edmeades, D.C. (1993) Effect of Various Metal Ions on Growth of Two Wheat Lines Known to Differ in Aluminium Tolerance. Plant and Soil, 155-156, 489-492.
http://dx.doi.org/10.1007/BF00025090
[29] Li, W., Khan, M.A., Yamaguchi, S. and Kamiya, Y. (2005) Effects of Heavy Metals on Seed Germination and Early Seedling Growth of Arabidopsis thaliana. Plant Growth Regulation, 46, 45-50.
http://dx.doi.org/10.1007/s10725-005-6324-2
[30] Shaikh, I.R., Shaikh, P.R., Shaikh, R.A. and Shaikh, A.A. (2013) Phytotoxic Effects of Heavy Metals (Cr, Cd, Mn and Zn) on Wheat (Triticum aestivum L.) Seed Germination and Seedlings Growth in Black Cotton Soil of Nanded, India. Research Journal of Chemical Science, 3, 14-23.
[31] Al-Qurainy, F. (2010) Application of Inter Simple Sequence Repeat (ISSR Marker) to Detect Genotoxic Effect of Heavy Metals on Eruca sativa (L.). African Journal of Biotechnology, 9, 467-474.
[32] Freeman, J.L. and Salt, D.E. (2007) The Metal Tolerance Profile of Thlaspi goesingense Is Mimicked in Arabidopsis thaliana Heterologously Expressing Serine Acetyl-Transferase. BMC Plant Biology, 7, 63. http://dx.doi.org/10.1186/1471-2229-7-63
[33] Nasr, N. (2013) Germination and Seedling Growth of Maize (Zea mays L.) Seeds in Toxicity of Aluminum and Nickel. Merit Research Journal of Environmental Science and Toxicology, 1, 110-113.
[34] Heidari, M. and Sarani, S. (2011) Effects of Lead and Cadmium on Seed Germination, Seedling Growth and Antioxidant Enzymes Activities of Mustard (Sinapis arvensis L.). ARPN Journal of Agricultural and Biological Science, 6, 44-47.
[35] Zhao, F.J., Jiang, R.F., Dunham, S.J. and McGrath, S.P. (2006) Cadmium Uptake, Translocation and Tolerance in the Hyperaccumulator Arabidopsis halleri. New Phytologist, 172, 646-654. http://dx.doi.org/10.1111/j.1469-8137.2006.01867.x
[36] Meng de, K., Chen, J. and Yang, Z.M. (2011) Enhancement of Tolerance of Indian Mustard (Brassica juncea) to Mercury by Carbon Monoxide. Journal of Hazardous Materials, 186, 1823-1829. http://dx.doi.org/10.1016/j.jhazmat.2010.12.062
[37] Shen, Q., Jiang, M., Li, H., Che, L.L. and Yang, Z.M. (2011) Expression of a Brassica napus Heme Oxygenase Confers Plant Tolerance to Mercury Toxicity. Plant Cell Environment, 34, 752-763.
http://dx.doi.org/10.1111/j.1365-3040.2011.02279.x
[38] Zulfiqar, A., Paulose, B., Chhikara, S. and Dhankher, O.P. (2011) Identifying Genes and Gene Networks Involved in Chromium Metabolism and Detoxification in Crambe abyssinica. Environmental Pollution, 159, 3123-3128.
http://dx.doi.org/10.1016/j.envpol.2011.06.027

  
comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.