Effect of Row Spacing on Seed Isoflavone Contents in Soybean [Glycine max (L.) Merr.]

Bobby Ragin¹, Masum Akond¹, Stella Kantartzi², Khalid Meksem², Harmin Herrera³, Cevdet Akbay³, David A. Lightfoot², My Abdelmajid Kassem^1,4*

¹Plant Genomics and Biotechnology Lab, Department of Biological Sciences, Fayetteville State University, Fayetteville, NC, USA.
²Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL, USA.
³Department of Chemistry and Physics, Fayetteville State University, Fayetteville, NC, USA.
⁴The School of Arts and Sciences, American University of Ras Al Khaimah, Ras Al Khaimah, United Arab Emirates.

DOI: 10.4236/ajps.2014.526418                

Abstract

Soybean isoflavones compounds such as genistein, daidzein, and glycitein have numerous human health benefits including the reduction of risks of cardiovascular diseases, breast and prostate cancers, and menaupose symptoms in women. Understanding the genetic and environmental control of isoflavones accumulation is of great importance for developing new cultivars with high amounts of seed isoflavones. This study was conducted to analyze the effect of row spacing (25 cm vs. 50 cm) on seed isoflavones accumulation using a recombinant inbred line (RIL) population derived from the cross of PI 438489B and “Hamilton” (PIxH, n = 50). The two row spaces generated plant densities of 250,000 plants/ha and 90,000 plants/ha, respectively. Significant differences in soybean seed isoflavones (daidzein, genistein and glycitein) contents have been observed between plants grown in the two different plant densities. The mean daidzein content was 0.03458 μg·g^-1 in plants grown in 50 cm row spaces (low plant density), which was significantly higher than its content (0.03019 μg·g^-1) in plants grown in 25 cm row spaces (high plant density). Similarly, the mean glycitein content in plants grown in 50 cm row spaces (0.01905 μg·g^-1) was significantly higher than its content in plants grown in 25 cm row spaces (0.00498 μg·g^-1. Also, the mean genistein content in plants grown in 50 cm row spaces (0.01466 μg·g^-1) was higher than its content in plants grown in 25 cm row spaces (0.00831 μg·g^-1). These preliminary results are important in guiding farmers and breeders on choosing the best row spaces to grow soybean plants in order to optimize isoflavones contents. Further studies are needed to understand the correlation between seed isoflavones contents and other agronomic traits such as seed yield, protein, and oil contents.

Keywords

Daidzein, Genistein, Glycitein, Row Space, Plant Density

Conflicts of Interest

The authors declare no conflicts of interest.

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