Partial Characterization of Bacteriocins from Two Pediococcus acidilactici Strains Isolated during Traditional Sorghum Beer Processing in Côte d’Ivoire

Solange Aka-Gbezo; Florent Kouadio N’Guessan; Théodore N’Dédé Djeni; Marcellin Koffi Djè; Bassirou Bonfoh

doi:10.4236/aim.2014.416135

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AiM> Vol.4 No.16, December 2014

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Partial Characterization of Bacteriocins from Two Pediococcus acidilactici Strains Isolated during Traditional Sorghum Beer Processing in Côte d’Ivoire

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DOI: 10.4236/aim.2014.416135 3,320 Downloads 4,008 Views Citations

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Solange Aka-Gbezo^1,2*, Florent Kouadio N’Guessan¹, Théodore N’Dédé Djeni¹, Marcellin Koffi Djè¹, Bassirou Bonfoh²

Affiliation(s)

¹UFR of Sciences and Food Technologies, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire.
²Centre Suisse de Recherches Scientifiques en Côte d’Ivoire (CSRS), Abidjan, Côte d’Ivoire.

ABSTRACT

Two lactic acid bacteria strains (At1BEAE22 and At344E21) isolated during tchapalo production were identified on the basis of phenotypic analyses. Bacteriocins produced by these strains were tested for their antimicrobial activities using well diffusion agar method. Heat resistance, pH sensitivity and enzyme treatments were also analyzed. Results showed that both lactic acid bacteria strains were identified as Pediococcus acidilactici. Their bacteriocins inhibited growth of Lactobacillus delbrueckii F/31, Listeria innocua ATCC 33090, Enterococcus faecalis, Enterococcus faecalis ATCC 29212, Streptococcus sp, Enterococcus faecalis CIP 105042 and Enterococcus faecium ATCC 51558. These bacteriocins were heat stable at 60°C for 30 min for all indicator bacteria. However, they remained active only against Lactobacillus delbrueckii and Listeria innocua at 121°C for 60 min. Moreover, they were active in a wide range of pH (3 to 9) with a maximum activity observed at pH 5 and 6 on all indicator bacteria. But, bacteriocin from Pediococcus acidilactici At34E21 was more stable at acidic pH than basic one. The fact that the bacteriocin was inactivated by proteinase K and α-chymotrypsin indicated its proteinaceous nature, a general characteristics of bacteriocins.

KEYWORDS

Bacteriocin, Pediococcus acidilactici, Lactic Acid Bacteria, Tchapalo Production

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Aka-Gbezo, S. , N’Guessan, F. , Djeni, T. , Djè, M. and Bonfoh, B. (2014) Partial Characterization of Bacteriocins from Two Pediococcus acidilactici Strains Isolated during Traditional Sorghum Beer Processing in Côte d’Ivoire. Advances in Microbiology, 4, 1250-1259. doi: 10.4236/aim.2014.416135.

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