Mice Heat Chamber Calibration ()
Paula Y. S. Poh1,2*,
Samuel Carus1,
Yifan Chen1,
Patricia A. Deuster1
1Department of Military and Emergency Medicine, Uniformed Services University of the Health Sciences,
Bethesda, MD, USA.
2University of Illinois at Urbana-Champaign, Urbana-Champaign, IL, USA.
DOI: 10.4236/jasmi.2014.44013
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Abstract
Background: Firm
conclusions regarding the etiology of heat exposure responses among animals are
difficult to draw due to different experimental designs and methodological
confounders, such as environmental chamber set-up and heating rates. The
purpose of this investigation was to 1) validate the heat test protocol for
mice models via cage location and orientation; and to 2) determine the maximum
number of cages that can be used without compromising individual heating rates.
Methods: A mice temperature transponder (G2 E-Mitter, Mini Meter, Respironics)
was centrally positioned inside each mice cage set in the environmental chamber
(Thermo Scientific Forma, Model 3961). Two cage orientations (adjacent,
left-to-right and parallel, front-to-back) with 3 set-ups (top shelf, bottom shelf
and both shelves) using 2 and 4 cages were examined in triplicate and averaged.
Transponders equilibrated at 21.5℃ for 5 min, then exposed to
39.5℃ for a minimum of 60 min. Results: A major finding was that adjacent (L-R) top
shelf set-up had the smallest temperature difference throughout the heat test
(Δ = 0.43℃ vs. Δ = 2.2℃)
and at minute 60 (Δ = 0.2℃ vs. Δ = 1.8℃).
Both orientations for the bottom shelf
set-up had a slower rise in temperature (0.04℃·min-1) than other set-ups (0.3℃·min-1). Using both shelves, top shelf cages were consistently warmer than
bottom shelf cages (1.0℃ - 3.6℃) for both orientations. Conclusions:
We strongly suggest using an adjacent (L-R) top shelf set-up since it enabled
uniform chamber heating rates and standardized heat exposure. Bottom shelf is
not recommended for use due to poor heating rate performance. Since an
increased number of cages may obstruct heat flow patterns, a one shelf set-up
with 2 cages should be used.
Share and Cite:
Poh, P. , Carus, S. , Chen, Y. and Deuster, P. (2014) Mice Heat Chamber Calibration.
Journal of Analytical Sciences, Methods and Instrumentation,
4, 95-101. doi:
10.4236/jasmi.2014.44013.
Conflicts of Interest
The authors declare no conflicts of interest.
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