Derivation Method for Determining Sorbitol in Fruit Trees

DOI: 10.4236/ajps.2014.523361   PDF   HTML   XML   3,748 Downloads   4,376 Views   Citations

Abstract

The goal of this study is to describe a methodology for the analysis of D-sorbitol using benzyl alcohol as derivatizing agent and liquid chromatography equipped with a diode-array detector (HPLC-DAD). The study was performed at the University of São Paulo State (UNESP), Ilha Solteira Campus, Brazil. The axillary buds of fruit trees were collected from the UNESP Teaching and Research Farm. Benzyl alcohol was used as derivatizing agent to extract D-sorbitol from the buds. Next, the D-sorbitol underwent solid phase extraction (SPE) using C18 cartridges and was then analyzed by liquid chromatography (HPLC-DAD). The HPLC-DAD derivatization method proposed showed excellent chromatography resolution and high accuracy in the separation of D-sorbitol (derivatized) for the axillary buds of avocado, black mulberry, Japanese pear and peach and also enabled eliminating the use of hazardous derivatizing agents.

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Canesin, R. , Isique, W. , Buzetti, S. and Souza, J. (2014) Derivation Method for Determining Sorbitol in Fruit Trees. American Journal of Plant Sciences, 5, 3457-3463. doi: 10.4236/ajps.2014.523361.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Stoop, J.M.H., Williamson, J.D. and Pharr, M. (1996) Manitol Metabolism in Plants: A Method for Coping with Stress. Trends in Plant Science, 1, 139-144. http://dx.doi.org/10.1016/S1360-1385(96)80048-3
[2] Negm, F.B. and Loeschier, W.H. (1981) Characterization and Partial Purification of Aldose-6-Phosphate Reductase (Alditol-6-Phosphate:NADP 1-Oxidoreductase) from Apple Leaves. Plant Physiology, 67, 139-142.
http://dx.doi.org/10.1104/pp.67.1.139
[3] Noiraud, N., Maurousset, L. and Lemoine, R. (2001) Transport of Polyols in Higher Plants. Plant Physiology Biochemistry, 39, 717-728. http://dx.doi.org/10.1016/S0981-9428(01)01292-X
[4] Belitz, H.D., Grosch, W. and Schieberle, P. (2009) Fruits and Fruit Products. Food Chemistry, 34, 807-861.
[5] Ball, G.F.M. (1990) The Application of HPLC to the Determination of Low Molecular Weight Sugars and Polyhydric Alcohols in Foods: A Review. Food Chemistry, 35, 117-152. http://dx.doi.org/10.1016/0308-8146(90)90026-Z
[6] Miyagi, M., Yokoyama, H. and Hibi, T. (2007) Sugar Microanalysis by HPLC with Benzoylation: Improvement via Introduction of a C-8 Cartridge and a High Efficiency ODS Column. Journal of Chromatography B, 854, 286-290.
http://dx.doi.org/10.1016/j.jchromb.2007.04.044
[7] Oehlke, J., Brudel, M. and Blasig, I.E. (1994) Benzylation of Sugars, Polyols and Amino Acids in Biological Fluids for High-Performance Liquid Chromatographic Analysis. Journal Chromatography B: Biomedical Science Applied, 655, 105. http://dx.doi.org/10.1016/0378-4347(94)00067-0
[8] Ribeiro, F.A.L., Ferreira, M.M.C., Morano, S.C., Da Silva, L.R. and Schneider, R.P. (2008) Validation Spreadsheet: A New Tool for Estimating the Analytical Figures of Merit for the Validation of Univariate Methods. Quimica Nova, 31, 164-171. http://dx.doi.org/10.1590/S0100-40422008000100029
[9] Meyer, V.R. (1996) Theoretical Principles. In: Practical High-Performance Liquid Chromatography, John Wiley & Sons, Hoboken, 17-45.
[10] International Conference on Harmonisation (ICH) (1995) Guidance for Industry Q2A: Text on Validation of Analytical Procedures.
[11] International Conference on Harmonisation (ICH) (1996) Guidance for Industry Q2B: Text on Validation of Analytical Procedures.
[12] Danzer, K. and Currie, L.A. (1998) Guidelines for Calibration in Analytical Chemistry. Pure and Applied Chemistry, 70, 993.
[13] Podder, S., Choudhury, J. and Roy, S. (2007) Secondary Benzylation with Benzyl Alcohols Catalyzed by a High-Valent Heterobimetallic Ir-Sn Complex. Journal of Organic Chemistry, 72, 3129-3132.
http://dx.doi.org/10.1021/jo0625094
[14] Muir, J.G., Rose, R.Y., Rosella, O., Liels, K., Barrett, J.S., Shepherd, S.J. and Gibson, P.R. (2009) Measurement of Short-Chain Carbohydrates in Common Australian Vegetables and Fruits by High-Performance Liquid Chromatography (HPLC). Journal Agricultural and Food Chemistry, 57, 554-565. http://dx.doi.org/10.1021/jf802700e
[15] Oura, Y., Yamada, K., Shiratake, K. and Yamaki, S. (2000) Purification and Characterization of a NAD+-Dependent Sorbitol Dehydrogenase from Japanese Pear Fruit. Phytochemistry, 54, 567-572.
http://dx.doi.org/10.1016/S0031-9422(00)00158-8
[16] Wrolstad, R.E. and Shallenberger, R.S. (1981) Free Sugars and Sorbitol in Fruits a Complilation from the Literature. Journal of the Association of Official Analytical Chemists, 61, 91-103.

  
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