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On the Question of Defining the Association Constants by the Method of Fluorescence Quenching

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DOI: 10.4236/ajac.2014.516113    3,096 Downloads   3,569 Views   Citations

ABSTRACT

A study is made on the previously ignored problem of the dependence of a static fluorescence quenching Stern-Volmer constant Ksv on the initial concentration of [F]0 fluorophore F. This correlation is shown to exist. It is concluded that the Stern-Volmer quenching constant may be used as association constant K only with .

Cite this paper

Lavrik, N. and Bazhin, N. (2014) On the Question of Defining the Association Constants by the Method of Fluorescence Quenching. American Journal of Analytical Chemistry, 5, 1065-1068. doi: 10.4236/ajac.2014.516113.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] De Weert, M. and Stella, L. (2011) Fluorescence Quenching and Ligand Binding: A Critical Discussion of a Popular Methodology. Journal of Molecular Structure, 998, 144-150.
http://dx.doi.org/10.1016/j.molstruc.2011.05.023
[2] Lakovicz, J. (2010) Principles of Fluorescence Spectroscopy. 3rd Edition, Springer, Berlin.

  
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