Effects of Zinc and Ascorbic Acid Application on the Growth and Photosynthetic Pigments of Millet Plants Grown under Different Salinity


Salinity stress impacts crop growth as well as production. The need for increased food production to feed the increasing population and the limited resources, i.e. optimal quality land and water, require developing strategies to mitigate marginal stresses, including salinity stress, for reasonable expectation of crop production. A pot experiment was conducted in a greenhouse at the National Research Centre, Dokki, Cairo, Egypt in the summer season of 2005 to evaluate the effects of foliar application of ascorbic acid alone or in combination with zinc sulfate on the growth and photosynthetic pigments of millet plants irrigated by tap water (250 ppm, 0.39 dS·m-1) or moderate to high salinity irrigation water [2500 ppm (3.9 dS·m-1) and 5000 ppm (7.8 dS·m-1)]. Increased salinity in the irrigation water decreased the plant growth, biomass, and carotenoid content. Foliar application of ascorbic acid alone increased number of leaves and leaf area, while in combination with zinc sulfate increased the plant height and total plant biomass. However, these treatments had no significant effects on the photosynthetic pigments. This study demonstrates that exogenous application of ascorbic acid can enhance foliar growth which may contribute to increased plant biomass and yield.

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Hussein, M. and Alva, A. (2014) Effects of Zinc and Ascorbic Acid Application on the Growth and Photosynthetic Pigments of Millet Plants Grown under Different Salinity. Agricultural Sciences, 5, 1253-1260. doi: 10.4236/as.2014.513133.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Khan A.A. and Abdullah, Z. (2003) Salinity-Sodicity Induced Changes in Reproductive Physiology of Rice (Oryza sativa L.) under Dense Soil. Environment and Experimental Botany, 47, 145-157.
[2] Heidari, M. and Jamshidi, P. (2011) Effects of Salinity and Potassium Application on Antioxidant Enzyme Activities and Physiological Parameters in Pearl Millet. Agricultural Sciences in China, 10, 228-237.
[3] Sastry, E.V.D., Sharma, H. and Sharma, H. (2000) Effect of Temperature and Salinity on the Germination and Seedling Growth in Wheat. Indian Journal of Agricultural Science, 70, 117-118.
[4] Hu, Y., Schmidhalter, U. and Hu, Y.C. (2001) Reduced Cellular Cross-Section Area in the Leaf Elongation Zone of Wheat Causes a Decrease in Dry Weight Deposition under Saline Conditions. Australian Journal of Plant Physiology, 28, 165-170.
[5] Akram, N., Hussein, M., Akhtar, S. and Rasul, E. (2002) Impact of NaCl Salinity on Yield Components of Some Wheat Accessions/Varieties. International Journal of Agriculture Biology, 4, 156-158.
[6] Hussein, M.M., El-Geratly, N.H. and Abo El-Khier, M.S. (2002) Endogenous Hormones, Growth and Yield of Barley Plants as Affected by Benzyl Adenine under Different Salinity Levels. Journal of Agricultural Science, Moshtohor, University, 27, 5283-5292.
[7] Hussein, M.M., Balbaa, L.H. and El-Liethy, S. (2007) The Effect of Saline Irrigation, Adenine Spraying and Their Interaction on the Growth and Photosynthetic Pigments in Barley. Egyptian Journal of Applied Science, 22, 173-186.
[8] Marschner, H. (1995) Mineral Nutrition of Higher Plants. 2nd Edition, Academic Press, London, 645.
[9] Younis, M.E., Hasaneen, M.N. and Kazamel, A.M. (2009) Plant Growth, Metabolism and Adaptation in Relation to Stress Conditions. XXVII. Can Ascorbic Acid Modify the Adverse Effects of NaCl and Mannitol on Amino Acids, Nucleic Acids and Protein Patterns in Viciafaba Seedlings? Protoplasma, 235, 37-47.
[10] Hussein, M.M. and El-Masry, M.F. (2007) Irrigation by Mixed Drainage Water and Micronutrients Spray and Its Effects on Micronutrients in Straw and Grains of Wheat. Proceedings of ICID 22nd European Regional Conference, Pavia, 2-7 September 2007, 89.
[11] Beltagi, M.S. (2008) Exogenous Ascorbic Acid (Vitamin C) Induced Anabolic Changes for Salt Tolerance in Chick Pea (Cicer arietinum L.) Plants. African Journal of Plant Science, 2, 118-123.
[12] Hussein, M.M., Shaaban, M.M. and El-Saady, A.M. (2008) Response of PK-Foliar Fertilizations Cowpea Plants Grown under Salinity Stress. American Journal of Plant Physiology, 3, 81-88.
[13] Von Wetstein, D. (1957) Chlorophyll Letale and Der Sub-Mikroskopishe Formweschselder Plastiden. Experimental Cell Research, 12, 427-506. http://dx.doi.org/10.1016/0014-4827(57)90165-9
[14] Snedecor, G.W. and Cochran, W.G. (1990) Statistical Methods. 8th Edition, Iowa State University Press, Ames.
[15] Manikandan, K. and Desingh, R. (2009) Effect of Salt Stress on Growth, Carbohydrate and Proline Content of Two Finger Millet Varieties. Recent Research in Science and Technology, 1, 48-51.
[16] Hussein, M.M., Tawfik, M.M., Ahmed, M.K. and El-Karamany, F. (2013) Effect of Water Stress on Growth and Some Physiological Aspects of Jojoba [Simmondisia chinieses (Link) Schenider] in New Reclaimed Sandy Soil. Elixer Pollution, 55, 12903-12909.
[17] Ashraf, M.Y., Akhtar, K., Sarwar, G. and Ashraf, M. (2003) Evaluation of Arid and Semi-Arid Ecotypes of Guar (Cyamopsis tetragonoloba L.) for Salinity (NaCl) Tolerance. Journal of Arid Environments, 52, 473-482.
[18] Olmos, E. and Hellín, E. (1996) Mechanisms of Salt Tolerance in a Cell Line of Pisum sativum: Biochemical and Physiological Aspects. Plant Science, 120, 37-45. http://dx.doi.org/10.1016/S0168-9452(96)04483-4
[19] Veeranagamallaiah, G., Jyothsnakumari, G., Thippeswamy, M., Reddy, P.C., Surabhi, G.K., Srirangayakulu, G., Mahesh, Y., Rajasekhar, B., Madhurarekha, Ch. and Sudhakar, C. (2008) Proteomic Analysis of Salt Stress Responses in Foxtail Millet (Setaria italica L. cv Prasad) Seedlings. Plant Science, 175, 631-641.
[20] Sheldon, A., Menzies, N.W., So, H.B. and Dalal, R. (2004) The Effect of Salinity on Plant Available Water. Proceedings of Australian New Zealand Soil Conference, University of Sydney, Sydney, 5-9 December 2004.
[21] Ragab, A.A., Hellal, F.A. and Abd El-Hady, M. (2008) Water Salinity Impacts on Some Soil Properties and Nutrients Uptake by Wheat Plants in Sandy and Calcareous Soils. Australian Journal of Basic and Applied Science, 2, 225-233.
[22] Kaya, C., Ashraf, M., Dikilitas, M. and Tuna, A.L. (2013) Alleviation of Salt Stress-Induced Adverse Effects of Maize Plants by Exogenous Application of Indoleacetic Acid (IAA) and Inorganic Nutrients. A Field Trial. Australian Journal of Crop Science, 7, 249-254.
[23] Pinheiro, H.A., Silva, J., Endres, L., Ferreira, V.M., Camara, C.A., Cabral, F.F., Oliveira, V.M., Carvalha, L.W., Santos, J.M. and Filho, B.G. (2008) Leaf Gas Exchange, Chloroplastic Pigments and Dry Matter Accumulation in Castor Bean (Ricinus communis L.) Seedlings Subjected to Salt Stress Conditions. Industrial Crops and Products, 27, 385-392. http://dx.doi.org/10.1016/j.indcrop.2007.10.003
[24] Tuna, A.L., Kaya, C., Dikilitas, M. and Higgs, D. (2008) The Combined Effects of Gibberelic Acid and Salinity on Some Antioxidant Enzymes Activity, Plant Growth Parameters and Nutritional Status in Maize Plants. Environmental and Experimental Botany, 62, 1-9. http://dx.doi.org/10.1016/j.envexpbot.2007.06.007
[25] Maslenkova, L.T., Miteva, T.S. and Popova, L.P. (1992) Changes in Polypeptide Patterns of Barley Seedlings Exposed to Jasmonic Acid and Salinity. Plant Physiology, 98, 700-707. http://dx.doi.org/10.1104/pp.98.2.700
[26] El-Tayeb, M.A. (2005) Response of Barley Grains to the Interactive Effect of Salinity and Salicylic Acid. Plant Growth Regulators, 45, 215-224. http://dx.doi.org/10.1007/s10725-005-4928-1
[27] Ahmadi, A. and Baker, D.A. (2001) The Effect of Water Stress on the Activities of Key Regulatory Enzymes of the Sucrose of Starch Pathway in Wheat. Plant Growth Regulation, 35, 81-91.
[28] Raza, S., Athar, H., Ashraf, M. and Hameed, A. (2007) Glycinebetaine-Induced Modulation of Antioxidant Enzymes Activities and Ion Accumulation in Two Wheat Cultivars Differing in Salt Tolerance. Environmental and Experimental Botany, 69, 368-376. http://dx.doi.org/10.1016/j.envexpbot.2006.12.009
[29] Ghanem, M.E., Albacete, A., Andújar, C.M., Acosta, M., Aranda, R.R., Dodd, I., Lutts, C. and Alfocea, F.P. (2008) Hormonal Changes during Salinity-Induced Leaf Senescence in Tomato (Solanum lycopersicum L.). Journal of Experimental Botany, 59, 3039-3050. http://dx.doi.org/10.1093/jxb/ern153
[30] Athar, H., Khan, A. and Ashraf, M. (2008) Exogenously Applied Ascorbic Acid Alleviates Salt-Induced Oxidative Stress in Wheat. Environmental and Experimental Botany, 63, 224-231.
[31] Abd El-Baky, H.H., Hussein, M.M. and Baroty, G.S. (2008) Algal Extraction Improve Antioxidants Defense Abilities and Salt Tolerance of Wheat Plant Irrigated with Sea Water. Electronic Journal of Environmental Agriculture and Food Chemistry, 7, 2812-832.
[32] Sairam, R.K. and Srivastava, G.C. (2002) Changes in Antioxidant Activity in Sub-Cellular Fractions of Tolerant and Susceptible Wheat Genotypes in Response to Long Term Salt Stress. Plant Science, 162, 897-904.
[33] Darwish, D.S., El-Gharreib, E.G., El-Hawary, M.A. and Rafft, O.A. (2002) Effect of Some Macro and Micronutrients Application on Peanut Production in a Saline Soil in El-Faiyum Governorate. Egyptian Journal of Applied Science, 17, 17-32.
[34] Gobarah, M.E., Mohamed, M.H. and Tawfik, M.M. (2006) Effect of Phosphorus Fertilizer and Foliar Spraying with Zinc on Growth, Yield and Quality of Groundnut under Reclaimed Sandy Soils. Journal of Applied Science Research, 2, 491-496.
[35] Thalooth, A.T., Badr, N.M. and Mohamed, M.H. (2005) Effect of Foliar Spraying with Zn and Different Levels of Phosphatic Fertilizer on Growth and Yield of Sunflower Plants Grown under Saline Condition. Egyptian Journal of Agronomy, 27, 11-22.
[36] Abd El-Moniem, A., Emman, A., Abd El-Mageed, M.M. and Omayma, M.M. (2007) GA3 and Zinc Sprays for Improving Yield and Fruit Quality of Washington Novel Orange Trees Grown under Sandy Conditions. Research Journal of Agriculture and Biological Science, 3, 498-395.
[37] Abd El-Aziz, N. Mazhar, A.M. and Habaa, E. (2006) Effect of Foliar Spray with Ascorbic Acid on Growth and Chemical Constituents of Kaya sengalensis Grown under Salt Condition. American-Eurasian Journal of Agriculture & Environmental Science, 1, 207-214.
[38] Ahmed-Hamed, A.M. and Monsaly, H.M. (1998) Seed Soak in Pre-Sowing in Vitamins versus the Adverse Effects of NaCl Salinity on Photosynthesis and Some Related Activities of Maize and Sunflower Plants. Proceedings of the XIth International Photosynthesis Conference, Budapest, August 1998, 17-22.
[39] Shalata, A. and Neumann, P.M. (2007) Exogenous Ascorbic Acid (Vitamin C) Increases Resistance to Salt Stress and Reduces Lipid Peroxidation. Journal of Experimental Botany, 52, 2207-2211.
[40] Verma, S. and Misra, N. (2005) Putrescine Alleviation of Growth in Salt Stressed Brassica juncia by Inducing Antioxidative Defense System. Journal of Plant Physiology, 162, 669-677. http://dx.doi.org/10.1016/j.jplph.2004.08.008
[41] Khan, T., Mazid, M. and Mohammad, F. (2011) A Review of Ascorbic Acid Potentialities against Oxidative Stress in Plants. Journal of Agrobiology, 28, 97-111. http://dx.doi.org/10.2478/v10146-011-0011-x

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