Synthesis and Characterization of Struvite-k Crystals by Agar Gel

Vilas Bhagawat Suryawanshi^*, Ravindranath Teniram Chaudhari

Physics Research Lab, Shri. V.S. Naik Arts, Commerce and Science College, Raver, India.

DOI: 10.4236/jcpt.2014.44026                

Abstract

The phosphate mineral struvite is basically formed in urinary tracks and kidney. One of the analogous compounds of struvite is potassium magnesium phosphate hexahydrate (KMgPO₄·6H₂O), known as struvite-k crystal and found in animal urinary calculi. In the present investigation, struvite-k crystals were grown by single diffusion and double diffusion techniques in agar gelmedium. The grown crystals were analyzed by optical microscopy, scanning electron microscopy (SEM), fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray analysis (EDXA) and thermogravimetric analysis (TGA). Optical microscopy and SEM exhibited the different morphologies. The FTIR spectra revealed the presence of water molecules, stretching and bending vibrations of phosphate (PO₄) ions. However the powder XRD results from the crystalline nature. Elemental composition in the crystal was obtained by EDXA, while 36.89% weight loss of water molecules is observed in TGA study.

Keywords

Gel Method, Agar Gel, Struvite-k, Animal Calculi

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Chauhan, C.K. and Joshi, M.J. (2013) In Vitro Crystallization, Characterization and Growth-Inhibition Study of Urinary Type Struvite Crystals. Journal of Crystal Growth, 362, 330-337. http://dx.doi.org/10.1016/j.jcrysgro.2011.11.008
[2]	Lingeman, J., Kahnoski, R., Mardis, H., Goldfarb, D.S., Grasso, M., Lacy, S., Scheinman, S.J., Asplin, J.R., Parks, J.H. and Coe, F.L. (1999) Divergence between Stone Composition and Urine Saturation: Clinical and Biological Implication. Journal of Urology, 161, 1077-1081. http://dx.doi.org/10.1016/s0022-5347(01)61594-5
[3]	Zarasvandi, A., Heidari, M., Sadeghi, M. and Mousapoor (2013) Major and Trace Element Composition of Urinary Stones, Khuzestan Province, Southwest, Iran. Journal of Geochemistry Exploration, 131, 52-58. http://dx.doi.org/10.1016/j.gexplo.2012.08.014
[4]	Kumar, N., Singh, P. and Kumar, S. (2006) Physical, X-Ray Diffraction and Scanning Electron Microscopic Studies of Uroliths. Indian Journal of Biochemistry& Biophysics, 43, 226-232.
[5]	Diana, K.J. and George, K.V. (2013) Urinary Stone Formation: Efficacy of Seed Extract of Ensetesuperbum (Roxb.) Cheesman on Growth Inhibition of Calcium Hydrogen Phosphatedihydrate Crystals. Journal of Crystal Growth, 363, 164-170. http://dx.doi.org/10.1016/j.jcrysgro.2012.10.036.
[6]	Parekh, B., Joshi, M. and Vaidya, A. (2008) Characterization and Inhibitive Study of Gel-Grown Hydroxyapatite Crystals at Physiological Temperature. Journal of Crystal Growth, 310, 1749-1753. http://dx.doi.org/10.1016/j.jcrysgro.2007.11.219
[7]	Menon, M., Parulkar, B.G. and Drach, G.W. (1998) Urinary Lithiasis: Etiology, Diagnosis and Medical Management, Campbell’s Urology, 3, 2661-2733.
[8]	Joshi, V.S. and Joshi, M.J. (2003) FTIR Spectroscopic, Thermal and Growth Morphological Studies of Calcium Hydrogen Phosphate Dihydrate Crystals. Crystal Research Technology, 38, 817-821. http://dx.doi.org/ 10.1002/crat.200310100
[9]	Abbona, F. and Boistelle, R. (1979) Growth Morphology and Crystal Habit of Struvite Crystals (MgNH4PO4·6H2O). Journal of Crystal Growth, 46, 339-354. http://dx.doi.org/10.1016/0022-0248(79)90082-4
[10]	Daeshner, C.W., Singleton, E.B. and Curtis, J.C. (1960) Urinary Tract Calculi and Nephrocalcinosis in Infants and Children. Journal of Pediatrics, 57-5, 721-732. http://dx.doi.org/ 10.1016/s0022-3476(60)80166-7
[11]	Sun, X., Shen, L., Cong, X., Zhu, H., Lv, J.L. and He, L. (2011) Infrared Spectroscopic Analysis of Urinary Stones (Including Stones Induced by Melamine-Contaminated Milk Powder) in 189 Chinese Children. Journal of Pediatric Surgery, 46, 723-728. http://dx.doi.org/10.1016/j.jpedsurg.2010.09.013
[12]	Alpay, H., Ozen, A., Gokee, I. and Biyikli, N. (2009) Clinical and Metabolic Features of Urolithiasis and Microlithiasis in Children. Pediatric Nephrology, 24, 2203-2209. http://dx.doi.org/ 10.1007/s00467-009-1231-9
[13]	Shah, A.M., Kalmunkar, S., Punekar, S.V., Billimoria, F.R., Bapat, S.D. and Deshmukh, S.S. (1991) Spectrum of Pediatric Urolithiasis in Western India. Indian Journal of Pediatrics, 58, 543-549. http://dx.doi.org/10.1007/BF02750939
[14]	Lonsdale, K. and Sutor, D.J. (1966) Newberyite in Ancient and Modern Urinary Calculi: Identification and Space Group. Science, 154, 1353-1354. http://dx.doi.org/10.1126/science.154.3754.1353
[15]	Chauhan, C.K., Joshi, M.J. and Vaidya, A.D.B.J. (2009) Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Commiphora wightii. Journal of Materials Science: Materials in Medicine, 20, 85-92. http://dx.doi.org/10.1007/s10856-008-3489-z
[16]	Jacobs, D., Heimbach, D. and Hesse, A. (2001) Chemolysis of Struvite Stones by Acidification of Artificial Urine—An in Vitro Study. Scandinavian Journal of Urology & Nephrology, 35, 345-349. http://dx.doi.org/10.1080/003655901753224387
[17]	Hesse, A. and Heimbach, D. (1999) Causes of Phosphate Stone Formation and the Importance of Metaphylaxis by Urinary Acidification: A Review. World Journal of Urology, 17, 308-315. http://dx.doi.org/10.1007/s003450050152
[18]	Babic-Ivancic, V., Kontrec, J. and Brecevic, L. (2004) Formation and Transformation of Struvite and Newberyite in Aqueous Solutions under Conditions Similar to Physiological. Urological Research, 32, 350-356. http://dx.doi.org/10.1007/s00240-004-0427-5
[19]	Banks, E., Chianelli, R. and Korenstein, R. (1975) Crystal Chemistry of Struvite Analogs of the Type MgMPO4·6H2O (M+ = K+, Rb+, Cs+, T1+, NH4+)1. Inorganic Chemistry, 14, 1634-1639. http://dx.doi.org/10.1021/ic50149a041
[20]	Ravikumar, R.V.S.S.N., Chandrasekhar, A.V., Rao, S.N., Madhu, N., Reddy, B.J. and Reddy, Y.P. (1999) Orthorhombic Site Symmetry of Cr3+ in ZnNH4PO4·6H2O Crystals. Crystal Research and Technology, 34, 911-914. http://dx.doi.org/10.1002/(SICI)1521-4079(199908)34:7<911::AID-CRAT911>3.0.CO;2-B
[21]	Chauhan, C.K., Vyas, P.M. and Joshi, M.J. (2011) Growth and Characterization of Struvite-K Crystals. Crystal Research and Technology, 46, 187-194. http://dx.doi.org/10.1002/crat.201000587
[22]	Zhang, S., Shi, H.S., Huang, S.W. and Zhang, P. (2013) Dehydration Characteristics of Struvite-K Pertaining to Magnesium Potassium Phosphate Cement System in Non-Isothermal Condition. Journal of Thermal Analysis and Calorimetry, 111, 35-40. http://dx.doi.org/10.1007/s10973-011-2170-9
[23]	Sun, W.D., Wang, J.Y., Zhang, K.C. and Wang, X.L. (2010) Study on Precipitation of Struvite and Struvite-K Crystal in Goats during Onset of Urolithiasis. Research in Veterinary Science, 88, 461-466. http://dx.doi.org/10.1016/j.rvsc.2009.11.010
[24]	Graeser, S., Postl, W., Bojar, H.P., Berlepsch, P., Armbruster, T., Raber, T., Ettinger, K. and Walter, F. (2008) Struvite-(K), KMgPO4·6H2O, the Potassium Equivalent of Struvite—A New Mineral. European Journal of Mineral, 20, 629-633.
[25]	Wang, X.L., Huang, K.H., Gao, J.B., Shen, X.Z., Lin, C.Y. and Zhang, G.D. (1997) Chemical Composition and Microstructure of Uroliths and Urinary Sediment Crystals Associated with the Feeding of High-Level Cottonseed Meal Diet to Water Buffalo Calves. Research in Veterinary Science, 62, 275-280. http://dx.doi.org/10.1016/S0034-5288(97)90204-6
[26]	Dunlevey, J. and Laing, M. (2008) Struvite Infection Calculi in Dogs: Problems with Urinary Calculus Identification, and the Value of the Results. South African Journal of Science, 104, 471-472. http://dx.doi.org/10.1590/S0038-23532008000600021
[27]	Patel, A.R. and Rao, A.V. (1982) Crystal Growth in Gel Media. Bulletin of Materials Science, 4, 527-548. http://dx.doi.org/10.1007/BF02824961
[28]	Shivkumar, G.R., Girija, E.K., Kalkura, S.N. and Subramanian, C. (1998) Crystallization and Characterization of Calcium Phosphates: Brushite and Monetite. Crystal Research and Technology, 33, 197-205. http://dx.doi.org/10.1002/(SICI)1521-4079(1998)33:2<197::AID-CRAT197>3.0.CO;2-K
[29]	Ouyang, J., Deng, S., Li, X., Tan, Y. and Bernd, T. (2004) Effects of Temperature and Sodium Carboxylate Additives on Mineralization of Calcium Oxalate in Silica Gel Systems. Science in China Series B, Chemistry, 47, 311-319. http://dx.doi.org/10.1360/03yb0027
[30]	Bretherton, T. and Rodgers, A. (1998) Crystallization of Calcium Oxalate in Minimally Diluted Urine. Journal of Crystal Growth, 192, 448-455. http://dx.doi.org/10.1016/s0022-0248(98)00461-8
[31]	Heferburg, G., Kloess, G., Schmitz, W. and Kothe, E. (2008) ‘‘Ni-Struvite”—A New Biomineral Formed by a Nickel Resistant Streptomyces Acidiscabies. Chemosphere, 72, 517-523. http://libgen.org/scimag4/10.1016/j.chemosphere.2008.02.050 http://dx.doi.org/10.1016/j.chemosphere.2008.02.050
[32]	Ravikumar, R.V.S.S.N., Chandrasekhar, A.V., Ramakrishna, C.H. and Reddy, Y.P. (2001) Structural and Spectral Studies of ZnKPO4·6H2O Crystals. Crystal Research and Technology, 36, 1429-1433. http://dx.doi.org/10.1002/1521-4079(200112)36:12<1429::AID-CRAT1429>3.0.CO;2-W
[33]	Henisch, H.K. (1988) Crystals in Gels and Liesegang Rings. Cambridge University Press, Cambridge. http://dx.doi.org/10.1017/CBO9780511525223
[34]	Bhavsar, D.S. (2012) Effect of Various Parameters to Grow Lead Iodide Crystals Grown by Gel Method. Archives of Physics Research, 3, 146-148.
[35]	Patil, S.N. and Venkateswara Rao, A. (1991) Effect of Gel Parameters on Nucleation and Growth of CaSO3·0.5H2O Crystals in Silica Gel. Crystal Research and Technology, 26, 847-853. http://dx.doi.org/10.1002/crat.2170260707
[36]	Dalal, P.V. and Saraf, K.B. (2011) Growth of Strontium Oxalate Crystals in Agar-Agar Gel. Bulletin of Materials Science, 34, 377-381. http://libgen.org/scimag2/10.1007/s12034-011-0080-x.pdf http://dx.doi.org/10.1007/s12034-011-0080-x
[37]	Bisaillon, S. and Tawashi, R. (1975) Growth of Calcium Oxalate in Gel Systems. Journal of Pharmaceutical Sciences, 64, 458-460. http://dx.doi.org/10.1002/jps.2600640325
[38]	Yellin, Z.B., Mil, J.V., Addadi, L., Idelson, M., Lahav, M. and Leiserowitz, L. (1985) Crystal Morphology Engineering by “Tailor-Made” Inhibitors: A New Probe to Fine Intermolecular Interactions. Journal of the American Chemical Society, 107, 3111-3122. http://dx.doi.org/10.1021/ja00297a017
[39]	Millan, A. (1997) Crystal Morphology and Texture in Calcium Oxalate Monohydrate Renal Calculi. Journal of Materials Science: Materials in Medicine, 8, 247-250. http://libgen.org/scimag3/10.1023/a%253A1018547909119.pdf
[40]	Yaghi, O.M., Li, G.M. and Li, H.L. (1997) Crystal Growth of Extended Solids by Non-Aqueous Gel Diffusion. Chemistry of Materials, 9, 1074-1076. http://dx.doi.org/ 10.1021/cm970069e
[41]	Dalal, P.V., Saraf, K.B. and Shah, S. (2009) Growth of Barium Oxalate Crystals in Agar-Agar Gel and Their Characterization. Crystal Research and Technology, 44, 36-42. http://dx.doi.org/10.1002/crat.200800221
[42]	Fost, R.L., Xi, Y., Scholz, R., Belotti, F.M. and Filho, M.C. (2013) The Phosphate Mineral Sigloite Fe3+Al2 (PO4)2(OH)3·7(H2O), an Exception to the Paragenesis Rule—A Vibrational Spectroscopic Study. Journal of Molecular Structure, 1033, 258-264. http://dx.doi.org/10.1016/j.molstruc.2012.10.020
[43]	Fost, R.L., Palmer, S., Xi, Y., Cejka, J., Sejkora, J. and Plasil, J. (2013) Raman Spectroscopic Study of the Hydroxy-Phosphate Mineral Plumbogummite PbAl3(PO4)2(OH,H2O)6. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 103, 431-434. http://dx.doi.org/10.1016/j.saa.2012.07.057
[44]	Fost, R.L., Cejka, J., Weier, M. and Martens, W.N. (2006) A Raman Spectroscopic Study of the Uranyl Phosphate Mineral Parsonsite. Journal of Raman Spectroscopy, 37, 879-891. http://dx.doi.org/10.1002/jrs.1517
[45]	Fost, R.L. and Palmer, S.J. (2007) A Raman Spectroscopic Study of the Phosphate Mineral Pyromorphite Pb5(PO4)3Cl. Polyhedron, 26, 4533-4541. http://dx.doi.org/10.1016/j.poly.2007.06.004
[46]	Reddy, B.J., Fost, R.L. and Palmer, S.J. (2007) A Near-Infrared Spectroscopic Study of the Phosphate Mineral Pyromorphite Pb5(PO4)3Cl. Spectrochimica Acta Part A, 71, 430-435. http://dx.doi.org/10.1016/j.saa.2007.12.030
[47]	Joint Committee for Powder Diffraction Standards Reference Card Number 35-0812.
[48]	Joint Committee for Powder Diffraction Standards Reference Card Number 01-075-1076.
[49]	Joint Committee for Powder Diffraction Standards Reference Card Number 20-0685.
[50]	Maleki, M., Ghamsari, M.S., Mirdamadi, Sh. and Ghasemzadeh, R. (2007) A Facile Route for Preparation of CdS Nanoparticles. Semiconductor Physics, Quantum Electronics & Optoelectronics, 10, 30-32.