Analysis of Tracheal Secretion in Healthy Horses Undergoing a Vaquejada Simulation Test

Natália Matos Souza Azevedo^1*, Márlon de Vasconcelos Azevedo¹, Paulo Fernandes de Lima¹, Hélio Cordeiro Manso Filho²

¹Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, Brazil.
²Departamento de Zootecnia, Universidade Federal Rural de Pernambuco, Recife, Brazil.

DOI: 10.4236/ojvm.2014.410027                

Abstract

This study aimed to evaluate the occurrence of EIPH in vaquejada horses via endoscopy and tracheal lavage and citology. We used eight Quarter-mile horses that usually perform at vaquejadas. Clinical examination was carried out before the race. After the race, endoscopy and tracheal lavage with 20 ml of saline via the service channel of the endoscope were performed. The samples were sent to the laboratory for processing and confection of the slides. Of the eight horses (four pull and four helper horses), six had hemosiderophages in their tracheal aspirates, confirming the existence of pulmonary hemorrhage after exercise. No difference was observed between the analyzed parameters between the two groups of vaquejada horses (P > 0.05). Tracheal lavage and endoscopy can be used for evaluating this important disease in animal athletes.

Keywords

EIPH, Horses, Sport, Endoscopy

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Wilsher, S., Allen, W.R. and Wood, J.L. (2006) Factors Associated with Failure of Thoroughbred Horses to Train and Race. Equine Veterinary Journal, 38, 113-118. http://dx.doi.org/10.2746/042516406776563305
[2]	Richard, E.A., Fortier, G.D., Lekeux, P.M. and Erck, E.V. (2010) Laboratory Findings in Respiratory Fluids of the Poorly-Performing Horse. The Veterinary Journal, 185, 115-122. http://dx.doi.org/10.1016/j.tvjl.2009.05.003
[3]	Hughes, K.J., Nicolson, L., Costa, N., Franklin, S.H., Allen, K.J. and Dunham, S.P. (2011) Evaluation of Cytokine mRNA Expression in Bronchoalveolar Lavage Cells from Horses with Inflammatory Airway Disease. Veterinary Immunology and Immunopathology, 140, 82-89. http://dx.doi.org/10.1016/j.vetimm.2010.11.018
[4]	McNamara, B., Bauer, S. and Lafe, J. (1990) Endoscopic Evaluation of Exercise Induced Pulmonary Haemorrhage and Chronic Obstructive Pulmonary Disease in Association with Poor Performance in Racing Standardbreds. Journal of American Veterinary Medical Association, 196, 443-445.
[5]	Burrell, M.H., Wood, J.L., Whitwell, K.E., Chanter, N., Mackintosh, M.E. and Mumford, J.A. (1996) Respiratory Disease in Thoroughbred Horses in training: The Relationships between Disease and Viruses, Bacteria and Environment. Veterinary Record, 139, 308-313. http://dx.doi.org/10.1136/vr.139.13.308
[6]	Wood, J.L.N., Newton, J.R., Chanter, N. and Mumford, J.A. (2005) Inflammatory Airway Disease, Nasal Discharge and Respiratory Infections in Young British Racehorses. Equine Veterinary Journal, 37, 236-242. http://dx.doi.org/10.2746/0425164054530579
[7]	Couetil, L.L., Rosenthal, F.S., De Nicola, D.B. and Chilcoat, C.D. (2001) Clinical Signs, Evaluation of Bronchoalveolar Lavage Fluid, and Assessment of Pulmonary Function in Horses with Inflammatory Respiratory Disease. American Journal of Veterinary Research, 62, 538-546. http://dx.doi.org/10.2460/ajvr.2001.62.538
[8]	Sanchez, A., Couetil, L.L., Ward, M.P. and Clark, S.P. (2005) Effect of Airway Disease on Blood Gas Exchange in Racehorses. Journal of Veterinary Internal Medicine, 19, 87-92. http://dx.doi.org/10.1111/j.1939-1676.2005.tb02663.x
[9]	Bailey, C.J., Reid, S.W., Hodgson, D.R. and Rose, R.J. (1999) Impact of Injuries and Disease on a Cohort of Two- and Three-Year-Old Thoroughbreds In training. Veterinary Record, 145, 487-493.
[10]	Moore, B.R., Krakowka, S., Robertson, J.T. and Cummins, J.M. (1995) Cytologic Evaluation of Bronchoalveolar Lavage Fluid Obtained from Standard Bred Racehorses with Inflammatory Airway Disease. American Journal of Veterinary Research, 56, 562-567.
[11]	Couetil, L.L., Hoffman, A.M., Hodgson, J., Buechner-Maxwell, V., Viel, L., Wood, J.L.N. and Lavoie, J.P. (2007) Inflammatory Airway Disease of Horses. Journal of Veterinary Internal Medicine, 21, 356-361. http://dx.doi.org/10.1111/j.1939-1676.2007.tb02975.x
[12]	Roberts, C., Hillidge, C. and Marlin, D. (1993) Exercise-Induced Pulmonary Haemorrhage in Racing Thoroughbreds in Great Britain. International EIPH Conference, Ontario, 21-22.
[13]	Pascoe, J.R., Ferraro, G.L., Cannon, J.H., Arthur, R.M. and Wheat, J.D. (1981) Exercise-Induced Pulmonary Hemorrhage in Racing Thoroughbreds: A Preliminary Study. American Journal of Veterinary Research, 42, 703-707.
[14]	Raphael, C.F. and Soma, L.R. (1982) Exercise-Induced Pulmonary Hemorrhage in Thoroughbreds after Racing and Breeding. American Journal of Veterinary Research, 43, 1123-1127.
[15]	Newton, J.R. and Wood, J.L. (2002) Evidence of an Association between Inflammatory Airway Disease and EIPH in Young Thoroughbreds during Training. Equine Veterinary Journal, 34, 417-424. http://dx.doi.org/10.1111/j.2042-3306.2002.tb05459.x.
[16]	Doucet, M.Y. and Viel, L. (2002) Clinical, Radiographic, Endoscopic, Bronchoalveolar Lavage and Lung Biopsy Findings in Horses with Exercise-Induced Pulmonary Hemorrhage. Canadian Veterinary Journal, 43, 195-202.
[17]	Dixon, P.M., Railton, D.I. and McGorum, B.C. (1995) Equine Pulmonary Disease: A Case Control Study of 300 Referred Cases. Part 1: Examination Techniques, Diagnostic Criteria and Diagnoses. Equine Veterinary Journal, 27, 416-421. http://dx.doi.org/10.1111/j.2042-3306.1995.tb04421.x
[18]	Hodgson, J.L. and Hodgson, D.R. (2003) Tracheal Aspirates: Indications, Technique and Interpretation. In: Robinson, N.E., Ed., Current Therapy in Equine Medicine, Saunders, St. Louis, 401-406. http://dx.doi.org/10.1016/B978-0-7216-9540-2.50130-4
[19]	Biava, J.S., Goncalves, R.C., Biondo, A.W. and Telles, J.E.Q. (2008) Escore total de hemossiderófagos (THS) na hemorragia pulmonar induzida por exercício (EIPH) em cavalos quarto de milha. Revista Acadêmica Ciências Agrárias Ambientais, 6, 335-340.
[20]	Holcombe, S.J. (2005) Epidemiology of Airway Inflammation and Mucus in Horses. American Association of Equine Practitioners, 51, 337-340.
[21]	Whitwell, K.E. and Greet, T.R.C. (1984) Collection and Evaluation of Tracheobronchial Washes in the Horse. Equine Veterinary Journal, 16, 499-508. http://dx.doi.org/10.1111/j.2042-3306.1984.tb02000.x
[22]	Cowell, R.L. and Tyler, R.D. (1992) Diagnostic Cytology and Hematology of the Horse. Mosby, St. Louis.
[23]	Hewson, J. and Viel, L. (2002) Sampling, Microbiology and Cytology of the Respiratory Tract. In: Lekeux, P., Ed., Equine Respiratory Diseases, Saunders, St. Louis.
[24]	Szenci, O., Orsolya K., Balogh N., Lajos Z. and Nagy K. (2011) Diagnostic Approaches for the Assessment of Equine Chronic Pulmonary Disorders. Journal of Equine Veterinary Science, 31, 400-410. http://dx.doi.org/10.1016/j.jevs.2011.03.018
[25]	Hodgson, J.L. and Hodgson, D.R. (2007) Collection and Analysis of Respiratory Tract Samples. In: McGorum, B.C., Dixon, P.M., Robinson, N.E. and Schumacher, J., Eds., Equine Respiratory Medicine and Surgery, Saunders, Philadelphia, 119-150. http://dx.doi.org/10.1016/B978-0-7020-2759-8.50014-3
[26]	Koblinger, K., Nicol, J., McDonald, K., Wasko, A., Logie, N., Weiss, M. and Léguillette, R. (2011) Endoscopic Assessment of Airway Inflammation in Horses. Journal of Veterinary Internal Medicine, 25, 1118-1126. http://dx.doi.org/10.1111/j.1939-1676.2011.00788.x
[27]	Viel, L. and Hewson, J. (2001) BAL Cytology in Horses with Exercise Intolerance: What Does It Tell Us? Proceedings of the 2nd World Equine Airways Symposium, Edinburgh, CD-ROM.
[28]	Sánchez, A., Couetil, L.L., Ward, M.P. and Clark, S.P. (2005) Effect of Airway Disease on Blood Gas Exchange in Racehorses. Journal of Veterinary Internal Medicine, 19, 87-92. http://dx.doi.org/10.1111/j.1939-1676.2005.tb02663.x
[29]	McKane, S.A., Canfield, P.J. and Rose, R.J. (2008) Equine Bronchoalveolar Lavage Cytology: Survey of Thoroughbred Racehorses in Training. Australian Veterinary Journal, 70, 401-404. http://dx.doi.org/10.1111/j.1751-0813.1993.tb06072.x
[30]	Araya, O., Palma, P., Salvi, M., Bustamante, H. and Vits, L. (2005) Endoscopic Determination of Exercise-Induced Pulmonary Haemorrhage in Chilean Criollo Horses. The Veterinary Journal, 169, 311-313. http://dx.doi.org/10.1016/j.tvjl.2004.02.002
[31]	Sweeney, C.R. and Humber, K.A. (1992) Cytologic Findings of Trachea-Bronchial Aspirates from 66 Thoroughbred Racehorses. American Journal of Veterinary Research, 53, 1172-1175.
[32]	Derksen, F.J., Brown, C.M., Sonea, I., Darien, B.J. and Robinson, N.E. (1989) Comparison of Transtracheal Aspirate and Bronchoalveolar Lavage Cytology in 50 Horses with Chronic Lung Disease. Equine Veterinary Journal, 21, 23-26. http://dx.doi.org/10.1111/j.2042-3306.1989.tb02084.x
[33]	Hoffman, A.M. (2008) Bronchoalveolar Lavage: Sampling Technique and Guidelines for Cytologic Preparation and Interpretation. Veterinary Clinics of North America: Equine Practice, 24, 423-435. http://dx.doi.org/10.1016/j.cveq.2008.04.003
[34]	Anderson, S., Shires, V.L., Wilson, R.A. and Mountford, A.P. (1999) Formation of Multinucleated Giant Cells in the Mouse Lung Is Promoted in the Absence of Interleukin-12. American Journal of Respiratory Cell and Molecular Biology, 20, 371-378. 10.1165/ajrcmb.20.3.3317
[35]	Helming, L. and Gordon, S. (2007) Macrophage Fusion Induced by IL-4 Alternative activation Is a Multistage Process Involving Multiple Target Molecules. European Journal of Immunology, 37, 33-42. http://dx.doi.org/10.1002/eji.200636788
[36]	Franklin, S.H., Naylor, J.R. and Lane, J.G. (2006) Videoendoscopic Evaluation of the Upper Respiratory Tract in 93 Sport Horses during Exercise Testing on a High-Speed Treadmill. Equine Veterinary Journal, 36, 540-545. http://dx.doi.org/10.1111/j.2042-3306.2006.tb05601.x
[37]	Davidson, E.J. and Martin, B.B. (2003) Diagnosis of Upper Respiratory Tract Diseases in the Performance Horse. Veterinary Clinics North American: Equine Practice, 19, 51-62. http://dx.doi.org/10.1016/S0749-0739(02)00066-4
[38]	Semeco, E., Rodrígues, M., Básalo, A., Aranguren, J. and Fernández, M. (2011) Prevalencia de las enfermidades obstructivas del tracto respiratorio superior em equinos Purosangres de Carreras. Revista Científica, 21, 215-223.
[39]	Erickson, H.H. and Poole, D.C. (2002) Exercise-Induced Pulmonary Hemorrhage. In: Lekeux, P., Ed., Equine Respiratory Diseases, Ithaca, New York.
[40]	Baccarin, R.Y.A. (2005) Diagnóstico e tratamento das pneumopatias de esforco. II Simpósio Internacional do Cavalo Atleta e IV Semana do Cavalo—SIMCAV, Belo Horizonte, 12-28.
[41]	Neto, J.B.A. (2009) Avaliacao endoscópica do trato respiratório superior e inferior proximal em cavalos de vaquejada. Ph.D. Thesis, Universidade Federal Rural de Pernambuco, Recife.