Pseudomonas aeruginosa BUP2—A Novel Strain Isolated from Malabari Goat Produces Type 2 Pyoverdine

Kizhakkepowathial Nair Unni¹, Prakasan Priji¹, Valerie A. Geoffroy², Mukesh Doble³, Sailas Benjamin^1*

¹Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India.
²Laboratoire de Microbiologie et de Génétique, CNRS UPRES-A 7010, Université Louis-Pasteur, Strasbourg, France.
³Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India.

DOI: 10.4236/abb.2014.511102                

Abstract

This study focuses on the isolation and characterization of a novel strain of siderophore producing bacterium, i.e., Pseudomonas aeruginosa BUP2 (Pa BUP2) from the rumen of Malabari goat, coupled with qualitative and quantitative analyses of the siderophore produced by it. Pa BUP2—a facultative anaerobe was tuned to be an aerobe by repeatedly growing in Benjamin flask. The new isolate was grown in a specially designed semi-synthetic medium, designated as BUP medium, and the yellowish-green pigment produced was identified as a typical siderophore by spectrophotometry, Chromazurol-S assay, thin layer chromatography and isolectric focusing (IEF). The characteristic orange fluorescence upon UV irradiation on chromatogram and absorption maximum at λ₄₀₄ confirmed that the characteristic siderophore produced by Pa BUP2 was a typical pyoverdine (PVD). This PVD was further categorized under type 2 by comparing its profile on the IEF gel with that of the representative strains of each PVD types, viz., Pa O1, Pa ATCC 27853 and Pa6. Moreover, the type 2 PVD was purified by XAD-4 Amberlite column chromatography and quantified; maximum yield (11.17 mg/ml) was observed on day 4 of incubation (37^°C). Thus, it was confirmed that the bacterium isolated from the rumen content of Malabari goat is a novel strain of Pa capable of producing large quantity of PVD type 2 in specially designed BUP medium under aerobic condition, and that its clinical and industrial implications remain elusive.

Keywords

Pseudomonas aeruginosa BUP2, Siderophore, Pyoverdine Type 2, Isoelectric Focusing

Conflicts of Interest

The authors declare no conflicts of interest.

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