Hemoglobin S Polymerization Effect on Water Self-Diffusion Coefficient


The transversal relaxation time, the effective transversal relaxation time and the water self-diffusion coefficient are evaluated during hemoglobin S polymerization. One homogeneous permanent magnet and one inhomogeneous and portable unilateral magnet with a very strong and constant static magnetic field gradient were utilized. The Carr-Purcell-Meiboom-Gill method was used before and after placing the studied samples 24 hours at 36°C to guarantee the polymerization. The transversal relaxation shows two exponents after polymerization supporting the concept of partially polymerized hemoglobin. The effective transversal relaxation time decreases around 40%, which can be explained by the increase of water self-diffusion coefficient 1.8 times as a main value. This result can be explained considering the effects of the agglutination process on the obstruction and hydration effects in a partially polymerized solution.

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Guevara, M. , García-Naranjo, J. , Mengana, Y. and Pereira, J. (2014) Hemoglobin S Polymerization Effect on Water Self-Diffusion Coefficient. Advances in Biological Chemistry, 4, 388-394. doi: 10.4236/abc.2014.46044.

Conflicts of Interest

The authors declare no conflicts of interest.


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