A Polymerase Chain Reaction Based DNA Fingerprinting Technique: Amplified Fragment Length Polymorphism for Molecular Typing of Perchlorate Reducing Bacteria

DOI: 10.4236/jep.2014.512109   PDF   HTML     2,402 Downloads   3,058 Views  

Abstract

Genetic profiling of environmentally important organisms is very essential for easy identification of biodegrading bacteria. In the previous study, we have reported the perchlorate biodegrading bacteria and characterized them by biochemical analysis and 16 S sequencing. We have observed a very similar isolates of Arthrobacter (Actinobacteria) degrading 4.1 mM and 4.7 mM of ammonium perchlorate [1]. In this study, we report PCR based DNA fingerprinting technique to generate the genomic signature of these closely related group of Arthrobacter species. This study also effectively generates unique genomic signature for each of these isolates that has potential for use in molecular monitoring as well as for tracking genomic variation and rearrangements.

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Shete, A. , Ghole, V. , Raina, M. and Aich, B. (2014) A Polymerase Chain Reaction Based DNA Fingerprinting Technique: Amplified Fragment Length Polymorphism for Molecular Typing of Perchlorate Reducing Bacteria. Journal of Environmental Protection, 5, 1111-1115. doi: 10.4236/jep.2014.512109.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Shete, A.M., Mukhopadhyaya, P.N., Acharya, A., Aich, B.A., Joshi, S. and Ghole, V.S. (2008) Aerobic Reduction of Perchlorate by Bacteria Isolated in Kerala, South India. Journal of Applied Genetics, 49, 425-431.
http://dx.doi.org/10.1007/BF03195643
[2] Skidmore, R., Leach, C., Hoffman, J., Amos, W. and Aldridge, D. (2010) Conservation Genetics of the Endangered Depressed River Mussel, Pseudanodonta complanata, Using Amplified Fragment Length Polymorphism (AFLP) Markers. Aquatic conservation: Marine and freshwater ecosystems. Aquatic Conservation: Marine and Freshwater Ecosystems, 20, 560-567.
http://dx.doi.org/10.1002/aqc.1131
[3] Lind, E.E. and Grahn, M. (2011) Directional Genetic Selection by Pulp Mill Effluent on Multiple Natural Populations of Three-Spined Stickleback (Gasterosteus aculeatus). Ecotoxicology, 20, 503-512.
http://dx.doi.org/10.1007/s10646-011-0639-8
[4] Geornaras, I., Kenene, N.F., von Holy, A. and Hastings, J.W. (1999) Amplified Fragment Length Polymorphism Fingerprinting of Pseudomonas Strains from a Poultry Processing Plant. Applied and Environmental Microbiology, 5, 3828-3833.
[5] Jackson, P.J., Hill, K.K., Laker, M.T., Ticknor, L.O. and Keim, P. (1999)Genetic Comparison of Bacillus anthracis and Its Close Relatives Using Amplified Fragment Length Polymorphism and Polymerase Chain Reaction Analysis. Journal of Applied Microbiology, 87, 263-269.
http://dx.doi.org/10.1046/j.1365-2672.1999.00884.x
[6] Janssen, P., Coopman, R., Huys, G., et al. (1996) Evaluation of the DNA Fingerprinting Method AFLP as a New Tool in Bacterial Taxonomy. Microbiology, 142, 1881-1893.
http://dx.doi.org/10.1099/13500872-142-7-1881
[7] Lin, J., Kuo, J. and Ma, J. (1996) A PCR-Based DNA Fingerprinting Technique: AFLP for Molecular Typing of Bacteria. Nucleic Acids Research, 4, 3649-3650.
http://dx.doi.org/10.1093/nar/24.18.3649
[8] Vos, P., Hogers, R., Bleeker, M., et al. (1995) AFLP: A New Technique for DNA Fingerprinting. Nucleic Acids Research, 23, 4407-4414.
http://dx.doi.org/10.1093/nar/23.21.4407
[9] Ajmone-Marsen, P., Valentini, A., Cassandro, M., Vecchiotti-Antaldi G., Bertoni, G. and Kuiper, M (1997) AFLP Markers for DNA Fingerprinting in Cattle. Animal Genetics, 28, 418-426.
http://dx.doi.org/10.1111/j.1365-2052.1997.00204.x
[10] Felsenstein, J. (2001) PHYLIP Phylogeny Inference Package. Version 3.6 (Alpha2), Department of Genetics, University of Wasington, Seattle.
[11] Zabeau, M. and Vos, P. (1993) Selective Restriction Fragment Amplification: Ageneral Method for DNA Fingerprinting. Publication 0 534 858 Al. European Patent Office, Munich.
[12] Vos, P. and Kuiper, M. (1996) AFLP. In: Caetano-Anolles, G. and Gresshoff, P.M., Eds., DNA Markers: Protocols, Applications and Overviews, J. Willey and Sons Inc., Bognor Regis.

  
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