A new risk assessment method for evaluation of oxidative chemicals using catalase mutant mouse primary hepatocytes

Da-Hong Wang, Yasuo Ishikawa, Masahiro Miyazaki, Hirofumi Fujita, Ken Tsutsui, Kuniaki Sano, Noriyoshi Masuoka, Keiki Ogino

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DOI: 10.4236/health.2011.35050                

Abstract

We examined the possibility of developing a new risk assessment method for potentially hazardous chemicals by using mouse primary hepatocytes from acatalasemic mice (Csb) and the wild-type (Csa) as predictive model. Chemical-induced cytotoxicities, such as hydrogen peroxide and lawsone, a main hair dye ingredient of henna, were examined. We observed the differences in cell survival between the Csa and Csb in a dose-dependant manner after treatment with either hydrogen peroxide or lawsone, supporting the usefulness of this newly established method for hazard identification of oxidative chemicals in a risk assessment process. More chemicals will be tested to confirm the usefulness of this method for the preliminary screening of oxidative chemicals before animal experimentation.

Keywords

Catalase-Mutant Mouse Hepatocytes; Reactive Oxygen Species; Hydrogen Peroxide; Hazard Assessment; Lawsone Cytotoxicity

Conflicts of Interest

The authors declare no conflicts of interest.

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