In Vitro Ovicidal and Larvicidal Activities of Stem Bark of Terminalia glaucescens (Combretaceae) against Haemonchus contortus


Objectives: To evaluate the in vitro activity of aqueous and methanolic extracts of stem bark of Terminalia glaucescens on the eggs and larval stages of Haemonchus contortus of sheep. Methods: The eggs were exposed for 24 hours in five different concentrations (625, 1250, 2500, 3750 and 5000 μg/ml) of methanol, hot water (decoction) and cold water extracts at room temperature (25°C). Distilled water, 1.25% DMSO and Albendazole were used as negatives and positive control respectively in the bioassay. Results: A significant effect was obtained with all of the three extracts and differences were observed depending on the parasitic stage. Hot water extract (HWE), Methanol (MET) and Cold water extract (CWE) inhibited embryonic development by 98.1%, 96% and 86.5% respectively at 5000 μg/ml, meanwhile Albendazole had a 77.9% inhibition. For the mean inhibition rate of egg hatching, there was a general increase in the inhibition rate with increase in concentration of the extract from 625 to 5000 μg/ml. Methanol extract had the highest inhibition rate in all concentration from 625 to 5000 μg/ml. Concerning larval mortality, HWE had the highest effect in L1 larval mortality in all concentration above 1250 μg/ml. Distilled water had very little larval mortality on L1 and L2 larvae by 3.3 and 3.3% respectively. Conclusion: This in vitro study brought out the ovicidal and larvicidal properties of stem bark of Terminalia glaucescens.

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Lem, M. , Vincent, K. , Josue, W. , Jeannette, Y. , Gertrude, M. and Joseph, T. (2014) In Vitro Ovicidal and Larvicidal Activities of Stem Bark of Terminalia glaucescens (Combretaceae) against Haemonchus contortus. American Journal of Plant Sciences, 5, 2859-2868. doi: 10.4236/ajps.2014.519301.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Aliasghar, T., Javad, J., Meysam, J., Farhang, S., Amirali, S., Mojtaba, R., Farshid, K., Hamid, A. and Mohammadreza, M. (2012) Histopathological Study of Haemonchus contortus in Herrik Sheep Abomasum. Bacteriology and Parasitology, 3.
[2] Miller, J.E. and Horohov, D.W. (2006) Immunological Aspects of Nematode Parasite Control in Sheep. Journal of Animal Science, 84, E124-E132.
[3] Githiori, J.B. (2004) Evaluation of Anthelmintic Properties of Ethnoveterinery Plants Preparations Used as Livestock Dewormers by Pastoralists and Small Holder Farmers in Kenya. Doctoral Dissertation, Department of Biomedical Sciences and Veterinary Public Health, SLU. Acta Universitatis Agriculture Sulciae, 76 p.
[4] Wabo Poné, J., Mpoame, M. and Bilong Bilong, C.F. (2009) In Vivo Evaluation of Potential Nematicidal Properties of Ethanol Extract of Canthium mannii (Rubiaceac) on Heligmosomoides polygyrus Parasite of Rodents. Veterinary Parasitology, 166, 103-107.
[5] Ngeh, J.T., Jacob, W., Mopoi, N. and Sali, D. (2007) Ethnoveterinary Medicine. A Practical Approach to the Treatment of Cattle Diseases in Sub-Saharan Africa. 2nd Edition, Agromisa Foundation and CTA, Wageningen, 88p.
[6] Wabo Poné. J., Billong Bilong, C.F. and Mpoame, M. (2010) In-Vitro Nematicidal Activity of Extracts of Canthium mannii (Rubiaceae), on Different Life-Cycle Stages of Heligmosomoides polygyrus (Nematoda, Heligmosomatidae). Journal of Helminthology, 84, 156-165.
[7] Wabo Poné, J., Kenne, T.F., Mpoame, M., Pamo, T.E. and Bilong Bilong, F. (2011) In-Vitro Activities of Acetonic Extract from Leaves of Three Forage Legumes (Calliandia calotysus, Gliricidia sepium and Leucaena diversifolia) on Haemonchus contortus. Asian Pacific Journal of Tropical Medicine, 112-420.
[8] Ciulei, I. (1982) Methodology for Analysis of Vegetable Drugs. Practical Manual on the Industrial Utilisation of Medicinal and Aromatic Plants. Bucharest, Romania, 1-62.
[9] Hansen, J. and Perry, B. (1990) The Epidemiology Diagnosis and Control of Gastrointestinal Parasite of Ruminants in Africa. Nairobi, 121p.
[10] Thienpont, D., Rochette, F. and Vanparljs, O.F.J. (1986) Diagnostic des Helminthoses par Examen Coprologique. Janssen Reseach Foundation, Beerse Belgium, 205p.
[11] ITDG and IIRR (1996) Ethnoveterinary Medicine in Kenya. A Field Manual of Traditional Animal Health Care Practices. Nairobi, Kenya. 226p.
[12] Symth, J.D. (1996) Animal Parasitology. 3rd Edition, Cambridge University Press, Great Britain, 549 p.
[13] D’Angelo, F., Poné, J.W., Yondo, J., Claire, K.M., Vittori, S., and Mbida, M. (2014) Evaluation of Ovicidal and Larvicidal Activities of Methylene Chloride Extract of Annona senegalensis (Annonaceae) Stem Bark on Heligmosomoides bakeri (Nematoda, Heligmosomatidae). Global Journal of Science Frontier Research, 14, 21-39.
[14] Dobson, R.J., Donal, A.D., Waller, P.J. and Snowdon, K.L. (1986) An Egg-Hatch Assay for Resistance to Levamisole in Trichostrongyloid Nematode Parasites. Veterinary Parasitology, 19, 77-84.
[15] Pessoa, L.M., Movais, S.M., Bevilaqua, C.M.L. and Luciano, J.H.S. (2002) Anthelmintic Activity of Essential Oil of Ocimum gratissimum L. and Euguenol against Haemonchus. Veterinary Parasitology, 24, 1-5.
[16] Wabo Poné, J., Bilong, C.F.B., Mpoame, M., Ngwa, C.F. and Coles, C.G. (2006) In Vitro Activity of Ethanol, Cold and Hot Water Extracts of the Stem Bark of Canthium mannii (Rubiaceae) on Ancylostoma caninum Eggs. East and Central African Journal of Pharmaceutical Sciences, 9, 14-18.
[17] Sinha, H.K., Sivastava, P.S., Singh, S.P., Sinha, K.V. and Sinha, S.R. (1987) Efficacy of Various Anthelmintics on the Mortality of the Infective Larva of Toxocara vitulorum and Treatment of Calf Ascariasis. Indian Journal of Animal Science, 57, 185-188.
[18] Hubert, J. and Kerboeuf, D. (1992) A Microlarval Development Assay for the Detection of Anthelmintic Resistance in Sheep Nematode. Veterinary Record, 130, 442-446.
[19] Kamtchouing, P., Kahpui, S.M., Djomeni, Dzeufiet, P.D., Tédong, L., Asongalem, E.A. and Dimo, T. (2006) Anti-Diabetic Activity of Methanol/Methylene Chloride Stem Bark Extracts of Terminalia superba and Canarium schweinfurthii on Streptozotocin-Induced Diabetic Rats. Journal of Ethnopharmacology, 104, 306-309.
[20] Adebayo, E.A. and Ishola, O.R. (2009) Phytochemical and Antimicrobial Screening of Crude Extracts from the Root, Stem Bark, and Leaves of Terminalia glaucescens. African Journal of Pharmacy and Pharmacology, 3, 217-221.
[21] Dongmo, A.B., Beppe, J.G., Tsabang, N. and Kamanyi, A. (2006) Analgesic Activities of the Stem Bark Extract of Terminalia superba (Engl. et Diels Combretaceae). Pharmacologyonline, 2, 171-177.
[22] Eguale, T., Tadesse, D. and Giday, M. (2011) In Vitro Anthelmintic Activity of Crude Extracts of Five Medicinal Plants against Egg-Hatching and Larval Development of Haemonchus contortus. Journal of Ethnopharmacology, 137, 108-113.
[23] Ademola, O.I. and Eloff, J.N. (2010) In Vitro Anthelmintic Activity of Combretum molle (R. Br. Ex G. Don) (Combretaceae) against Heamonchus contortus Ova and Larvae. Veterinary Parasitology, 169, 198-203.
[24] Argentieri, P.M., D’Addabbe, T., Tava, A., Agostinelli, A., Jurzysta, M. and Anato, P. (2008) Evaluation of Nematicidal Properties of Saponins from Medicago sativa spp. European Journal of Plant Pathology, 120, 189-197.
[25] Hoste, H., Jackson, F., Athanasiadou, S., Thamsbong, S.M. and Hoskin, S.O. (2006) The Effects of Tannin-Rich Plants on Parasitic Nematodes in Ruminants. Trends in Parasitology, 22, 253-261.

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