Total Reflection X-Ray Fluorescence Analysis of Toxic Metals in Fish Tissues

Graciela Zarazúa, Karina Girón-Romero, Samuel Tejeda, Carmen Carreño-De León, Pedro Ávila-Pérez

Faculty of Science, University of Mexico State, Tlachaloya, Mexico.
Nuclear Technology Division, National Institute for Nuclear Research, Ocoyoacac, Mexico.
Technology Institute of Toluca, Metepec, Mexico.

DOI: 10.4236/ajac.2014.512089                

Abstract

The concentration of Cr, Ni, Cu, Zn, Hg and Pb, considered like toxic, was evaluated in fish tissues by means the total reflection X-Ray Fluorescence technique (TXRF). Grass carp (Ctenopharygodon idella) samples were collected from six regions of the Tepuxtepec Dam which was used as a water reservoir and fish production for the local population. Fish tissues: gills, liver, kidney and muscle were analyzed in triplicate using a TXRF spectrometer “TX-2000 Ital Structures” with a Si(Li) detector and a resolution of 140 eV (FWHM) at Mn Kα and Mo tube (40 kV, 30 mA) with 17.4 KeV excitation energy. The mean concentration of metals in tissues decrease in the sequence: Kidney ≈ Gills > Liver > Muscle; the range of concentration for Zn was 26.68 - 816.67 mg/Kg followed by Cu: 1.18 - 35.81 mg/Kg, Cr: 1.10 - 9.30 mg/Kg, Ni: 0.40 - 3.80 mg/Kg, Pb: <0.02 - 1.92 mg/Kg and Hg: <0.03 - 1.97 mg/Kg. Chromium and mercury concentrations slightly exceed the limits for fish proposed by FAO/WHO and Mexican Legislation for human consumption in the edible parts of fish from each one of the regions of the Tepuxtepec Dam. The results show that metal accumulation in herbivorous carp tissues was higher in regions 1, 2 and 3 than the others, which could be due to the influence of Lerma River, one of the most polluted rivers of Mexico.

Keywords

Metals, Fish Tissues, TXRF

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Tejeda, S., ávila-Pérez, P. and Zarazúa, G. (2011) Evaluación del índice de la calidad de agua en el curso alto del río Lerma. In: Bedregal, P., Ed., Propuesta de un índice de calidad de agua para la región de Latinoamérica y el Caribe, Peru, 147-164.
[2]	Fatima, M. and Usmani, N. (2013) Histopathology and Bioaccumulation of Heavy Metals (Cr, Ni and Pb) in Fish (Channa striatus and Heteropneustes fossilis) Tissue: A Study for Toxicity and Ecological Impacts. Pakistan Journal of Biological Sciences, 16, 412-420. http://dx.doi.org/10.3923/pjbs.2013.412.420
[3]	Mansour, S. and Sidky, M. (2002) Ecotoxicological Studies. 3. Heavy Metals Contaminating Water and Fish from Fayoum Governorate Egypt. Food Chemistry, 78, 15-22. http://dx.doi.org/10.1016/S0308-8146(01)00197-2
[4]	Papagiannis, I., Kagalou, I., Leonardos, J., Petridis, D. and Kalfakaou, V. (2004) Copper and Zinc in Four Freshwater Fish Species from Lake Pamvotis (Greece). Environment International, 30, 357-362. http://dx.doi.org/10.1016/j.envint.2003.08.002
[5]	Rao, L. and Padmaja, G. (2000) Bioaccumulation of Heavy Metals in M. cyprinoids from the Harbor Waters of Visakhapatnamu. Bulletin Applied Science, 19, 77-85.
[6]	Lloyd, R. (2003) Pollution and Freshwater Fish. Fishing New Books Ed., Oxford.
[7]	ávila-Pérez, P., Zarazúa, G., Carapia-Morales, L., Tejeda, S., Barceló-Quintal, I. and Martínez, T. (2011) Concentration of Elements in Suspended Matter Discharges to Lerma River, Mexico. Journal of Radioanalytical and NuclearChemistry, 287, 383-391. http://dx.doi.org/10.1007/s10967-010-0887-8
[8]	FAO/WHO, Comission of Codex Alimentarius (2004) Programa Conjunto FAO/OMS Sobre Normas Alimentarias ALINORM 04/27/18. Pescado y Productos Pesqueros, Roma.
[9]	SS: Secretaría de Salud, Bienes y Servicios (1995) Productos de la pesca. Pescados frescos, refrigerados y congelados. Norma Oficial Mexicana NOM-O27-SSA1-1993.
[10]	SEMARNAT: Secretaría del Medio Ambiente y Recursos Naturales (2006) Acuerdo por el que se da a conocer el estudio técnico de los recursos hídricos del área geográfica Lerma-Chapala. Diario Oficial de la Federación, 24 Julio, México.
[11]	Dybem, B. (1993) Field Sampling and Preparation Subsamples of Aquatic Organism for Analysis Metals and Organochlorides, FAO. Fishery Technology, 212, 1-13.
[12]	Deutsches Institut für Normung E.V. (2001) Total Reflection X-Ray Fluorescence Analysis (TXRF). German Norm: E DIN 51003:2001-01.
[13]	Ital-Structures (2000) TX-2000 Spectrometer: Guide to the User, Italy.
[14]	Klockenkamper, R. (1997) Total-Reflection X-Ray Fluorescence Analysis. Wiley, New York.
[15]	Vinodhini, R. and Narayanan, M. (2008) Bioaccumulation of Heavy Metals in Organs of Fresh Water Fish Cyprinus carpio (Common Carp). International Journal of Environmental Science and Technology, 5, 179-182. http://dx.doi.org/10.1007/BF03326011
[16]	Hogstrand, C. and Haux, C. (1991) Binding and Detoxification of Heavy Metals in Lower Vertebrates with Reference to Metallothionein. Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 100, 137-141. http://dx.doi.org/10.1016/0742-8413(91)90140-O
[17]	Jaffar, J. and Pervaiz, S. (1989) Investigation of Multiorgan Heavy Trace Metal Content of Meat of Selected Dairy, Poultry, Fowl and Fish Species Pakistan. Journal of Scientific and Industrial Research, 32, 175-177.
[18]	Shriver, D.F., Atkins, P.W. and Longford, C.H. (1989) Inorganic Chemistry. Oxford University Press, Oxford.
[19]	Kendrick, M.H., Moy, M.T., Plishka, M.J. and Robinson, K.D. (1992) Metals and Biological Systems. Ellis Horwood Ltd., Chichester.
[20]	Heath, A.G. (1987) Water Pollution and Fish Physiology. CRC Press, Boca Raton.
[21]	Sen, I., Shandil, A. and Shrivastava, V.S. (2012) Study for Determination of Heavy Metals in Fish Species of the River Yamuna (Delhi) by Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). Advances in Applied Science Research, 2, 161-166.
[22]	Agatha, N. (2011) Levels of Some Heavy Metals in Tissues of Bonga Fish, Ethmallosa fimbriata (Bowdich, 1825) from Forcados River. Journal of Applied Environmental and Biological Sciences, 1, 44-47.
[23]	Yacoub, A.M. and Gad, N.S. (2012) Accumulation of Some Heavy Metals and Biochemical Alterations in Muscles of Oreochromis niloticus from the River Nile in Upper Egypt. International Journal of Environmental Science and Technology, 3, 1-10. http://www.pvamu.edu/texged
[24]	Pagenkopf, G.K. (1983) Gill Surface Interaction Model for Trace Metal Toxicity of Fish. Role of Complexation, pH, Water Hardness. Environmental Science and Technology, 17, 342-347.