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Effect of Dissolved Oxygen and Inoculum Concentration on Xylose Reductase Production from Candida guilliermondii Using Sugarcane Bagasse Hemicellulosic Hydrolysate

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DOI: 10.4236/fns.2011.23033    5,360 Downloads   9,248 Views   Citations

ABSTRACT

This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate. Both the parameters were studied under an experimental design 22 with triplicate at central point. The statistical analysis of the results indicated a significant negative effect on XR production from the variable inoculum. The variable dissolved oxygen also showed a negative effect on XR production. We found the maximum enzyme activity (2.5 U?mg?1) when both the factors were applied at their lowest levels. The yeast showed to be potentially capable for xylose reductase production when sugarcane bagasse hemicellulosic hydrolysate was used as carbon source. Also, the results presented important information for further optimization of xylose reductase attainment.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

T. Santos Milessi, A. Chandel, R. Freitas Branco and S. da Silva, "Effect of Dissolved Oxygen and Inoculum Concentration on Xylose Reductase Production from Candida guilliermondii Using Sugarcane Bagasse Hemicellulosic Hydrolysate," Food and Nutrition Sciences, Vol. 2 No. 3, 2011, pp. 235-240. doi: 10.4236/fns.2011.23033.

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