Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

Hanen Chammem, Imen Hafaid, Olivier Meilhac, Farid Menaa, Laurence Mora, Adnane Abdelghani

Carthage University, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Charguia, Tunisia.
Carthage University, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Charguia, Tunisia.
Department of Pharmaceutical Sciences and Nanomedicine, Fluorotronics USA, Inc., San Diego, USA.
INSERM UMR698, Paris, France.
Université Paris 13, Sorbonne Paris Cité, Villetaneuse, France.

DOI: 10.4236/jbnb.2014.53018                

Abstract

In this work, we describe an approach of detecting biomarkers by Surface Plasmon Resonance imaging (SPRi) technique in real samples. Two C-Reactive Protein (CRP)-antibody immobilization methods were used: The first method was based on direct physisorption of CRP-antibody onto gold surface; the second one was based on oriented CRP-antibody with protein G intermediate layer. The two developed immunosensors were tested against CRP antigen in phosphate buffer saline solution with the SPRi technique. The response of the developed immunosensors was reproducible and stable. The detection limit of 10 pg·mL^-1 and 50 pg·mL^-1 CRP-antigen was observed with and without protein G respectively with this technique. Moreover, the developed SPRi immunosensor was used for CRP-antigen detection in human plasma. A detection limit of 5 ng·mL^-1 and 10 ng·mL^-1 was obtained with and without protein G respectively. These obtained results were compared to those obtained with QCM (Quartz Crystal Microbalance) and Enzyme-Linked Immunosorbent Assay (ELISA) techniques.

Keywords

C-Reactive Protein, Protein G, Surface Plasmon Resonance Imaging, Human Plasma

Conflicts of Interest

The authors declare no conflicts of interest.

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