Bioethanol Production from Rice Straw Enzymatically Saccharified by Fungal Isolates, Trichoderma viride F94 and Aspergillus terreus F98

Mervate A. Abo-State, Ahmed M. E. Ragab, Nour Sh. EL-Gendy, Laila A. Farahat, Hekmat R. Madian

Egyptian Petroleum Research Institute (EPRI), Cairo, Egypt.
Faculty of Science (Girls Branch), Al-Azhar University, Cairo, Egypt.
National Center for Radiation Research and Technology (NCRRT), Cairo, Egypt.

DOI: 10.4236/soft.2014.32003                

Abstract

Egypt faces a high population growth rate nowadays, which demands for an increase in agricultural production efficiency. Consequently, agricultural field residues will increase. Rice straw is one of the main agriculture residues in Egypt. So this study was performed on rice straw as a resource for production of bioethanol. Eight microbial isolates, five yeasts and three fungi were isolated from rice straw. Yeast isolates were selected for their ability to utilize different sugars and cellulose. Chipped and grinded rice straw was subjected to different pretreatment methods physically through steam treatment by autoclaving and different doses of gamma γ irradiation (50 and 70 Mrad). Autoclaved pretreated rice straw was further enzymatically treated throughout solid state fermentation process by different fungal isolates; F68, F94 and F98 producing maximum total reducing sugars of 12.62, 13.58, 17.00 g/L, respectively. Bioethanol production by separate microbial hydrolysis and fermentation (SHF) process of rice straw hydrolysate was performed by the two selected fungal isolates; Trichoderma viride F94 and Aspergillus terreus F98 and two yeast isolates (Y26 and Y39). The two yeast isolates have been identified by 18S, RNA as Candida tropicalis Y26 and Saccharomyces cerevisiae Y39. SHF processes by F94 and Y26 produced 45 gallon/ton rice straw while that of F98 and Y39 produced 50 gallon/ton rice straw.

Keywords

Bioethanol, Rice Straw, Different Pretreatments, Saccharomyces cerevisiae, Candida tropicalis, Separate Hydrolysis and Fermentation (SHF)

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Moiser, N., Wyman, C., Dale, B., Elander, R., Lee, Y.Y. and Holtzapple, M. (2005) Features of Promising Technologies for Pretreatment of Lignocellulosic Biomass. Bioresource Technology, 96, 673-686. http://dx.doi.org/10.1016/j.biortech.2004.06.025
[2]	Masud, H.Sk., Anantharaman, N. and Manas, D. (2012) Bioethanol Fermentation from Untreated and Pretreated Lig- nocellulosic Wheat Straw Using Fungi Fusarium oxysporum. Indian Journal of Chemical Technology, 19, 63-70.
[3]	Park, I., Kim, I., Kang, K., Sohn, H., Rhee, I., Jin, I. and Jang, H. (2010) Cellulose Ethanol Production from Waste Newsprint by Simultaneous Saccharification and Fermentation Using Saccharomyces cerevisiae KNU5377. Process Biochemistry, 45, 487-492. http://dx.doi.org/10.1016/j.procbio.2009.11.006
[4]	Claassen, P.A.M., Van Lier, J.B., Lopez Contreras, A.M., Van Niel, E.W., Sijtsma, L., Stams, A.J.M., de Vries, S.S. and Weusthuis, R.A. (1999) Utilization of Biomass for the Supply of Energy Carriers. Applied Microbiology and Bio- technology, 52, 741-755. http://dx.doi.org/10.1007/s002530051586
[5]	Manjunath, S.G. and Geeta, G.S. (2007) Effectiveness of Fungal Pretreatment of Agro Residues on Ethanol Production by Yeasts and Zymomonas mobilis. Karnataka Journal of Agricultural Sciences, 20, 301-304.
[6]	Rabelo, S.C., Carrere, H., Maciel Filho, R. and Costa, A.C. (2011) Production of Bioethanol, Methane and Heat from Sugarcane Bagasse in a Biorefinery Concept. Bioresource Technology, 102, 7887-7895. http://dx.doi.org/10.1016/j.biortech.2011.05.081
[7]	Chum, H.L. and Overend, R.P. (2001) Biomass and Renewable Fuels. Fuel Processing Technology, 71, 187-195. http://dx.doi.org/10.1016/S0378-3820(01)00146-1
[8]	Karimi, K., Emtiazi, G. and Taherzadeh, M.J. (2006) Ethanol Production from Dilute Acid Pretreated Rice Straw by Simultaneous Saccharification and Fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae. Enzyme and Microbial Technology, 40, 138-144. http://dx.doi.org/10.1016/j.enzmictec.2005.10.046
[9]	Sabaa, M.F. and Sharaf, M.F. (2000) Egyptian Policies for Rice Development. Cahiers Options Mediterraneennes, 40, 25-36.
[10]	Sherief, A.A., El-Tanash, A.B. and Temraz, A.M. (2010) Lignocellulolytic Enzymes and Substrate Utilization during Growth and Fruiting of Pleurotus ostreatus on Some Solid Wastes. Journal of Environmental Science and Technology, 3, 18-34. http://dx.doi.org/10.3923/jest.2010.18.34
[11]	McAloon, A., Taylor, F., Yee, W., Ibsen, K. and Wooley, R. (2000) Determining the Cost of Producing Ethanol from Corn Starch and Lignocellulosic Feedstocks. Technical Report NREL/TP-580-28893.
[12]	Quintero, J.A., Montoya, M.I., Sánchez, O.J., Giraldo, O.H. and Cardona, C.A. (2007) Fuel Ethanol Production from Sugarcane and Corn: Comparative Analysis for a Colombian Case. Energy.
[13]	Aden, A., Ruth, M., Ibsen, K., Jechura, J., Neeves, K., Sheehan, J., Wallace, B., Montague, L., Slayton, A. and Lukas, J. (2002) Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Concurrent Dilute Acid Pre- Hydrolysis and Enzymatic Hydrolysis for Corn Stover. Technical Report NREL/TP-510-32438, National Renewable Energy Laboratory, Golden, 143 p. http://dx.doi.org/10.2172/15001119
[14]	Sánchez, O.J., Fraga, E.S. and Cardona, C.A. (2006) Process Synthesis for Fuel Ethanol Production from Lignocellu- losic Biomass Using an Optimization-Based Strategy. World Renewable Energy Congress IX and Exhibition, Florence.
[15]	Mutalik, S., Vinodkumar, C.S., Swamy, S. and Manjappa, S. (2011) Depolymerization of Bagasse by Ruminococcus albus in the Production of Eco-Friendly Fuel. Research in Biotechnolgy, 2, 1-6.
[16]	Patel, J., Seema, D., Ronkarappa, R. and Shobha, K.S. (2007) Fungal Pretreatment Studies on Rice Husk and Bagasse for Ethanol Production. Electronic Journal of Environmental, Agricultural and Food Chemistry, 6, 1921-1926.
[17]	Goshadrou, A., Karimi, K. and Taherzadeh, M.J. (2001) Bioethanol Production from Sweet Sorghum Bagasse by Mu- cor hiemalis. Industrial Crops and Products, 34, 1219-1225.
[18]	Martín, C., Galbe, M., Wahlbom, C.F., Hahn-H?gerdal, B. and J?nsson, L.J. (2002) Ethanol Production from Enzyma- tic Hydrolysates of Sugarcane Bagasse Using Recombinant Xylose-Utilizing Saccharomyces cerevisiae. Enzyme and Microbial Technology, 31, 274-282. http://dx.doi.org/10.1016/S0141-0229(02)00112-6
[19]	Abo-State, M.A.M., Hammad, A.I., Swelim, M. and Gannam, R.B. (2010) Enhanced Production of Cellulase (s) by Aspergillus spp. Isolated from Agriculture Wastes by Solid State Fermentation. American-Eurasian Journal of Agri- cultural & Environmental Sciences, 8, 402-410.
[20]	Wickerhams, L.J. (1951) Taxonomy of Yeasts. US Department of Agriculture, Washington DC, Technical Bulletin No.1029, 1-56.
[21]	Oxoid (1982) Manual of Culture Media, Ingredients and Other Laboratory Services. Oxoid Limited, Basingstoke.
[22]	Bahkali, A.H. (1995) Production of Cellulase, Xylanase and Polygalacturonase by Verticillium tricorpusaon on Dif- ferent Substrates. Bioresource Technology, 51, 171-174. http://dx.doi.org/10.1016/0960-8524(94)00110-M
[23]	Abo-State, M.A.M. (2003) Production of Carboxymethyl Cellulase by Fusarium oxysporium and Fusarium neoceros from Gamma-Pretreated Lignocellulosic Wastes. Egyptian Journal of Biotechnology, 15, 151-168.
[24]	Miller, G.L. (1959) Use of Dinitrosalicylic Acid Reagent for the Determination of Reducing Sugars. Analytical Chemi- stry, 31, 426-428. http://dx.doi.org/10.1021/ac60147a030
[25]	Uma, C., Muthulakshmi, C., Gomathi, D. and Gopalakrishnan, V.K. (2010) Fungal Invertase as Aid for Production of Ethanol from Sugarcane Bagasse. Research Journal of Microbiology, 5, 980-985. http://dx.doi.org/10.3923/jm.2010.980.985
[26]	Yu, Z.S. and Zhang, H.X. (2004) Ethanol Fermentation of Acid-Hydrolyzed Cellulosic Pyrolysate with Saccharomyces cerevisiae. Bioresource Technology, 93, 199-204. http://dx.doi.org/10.1016/j.biortech.2003.09.016
[27]	Gunasekaran, P. and Kamini, N.R. (1991) High Ethanol Productivity from Lactose by Immobilized Cells of Kluyvero- myces fragilis and Zymomonas mobilis. World Journal of Microbiology and Biotechnology, 7, 551-556. http://dx.doi.org/10.1007/BF00368359
[28]	Pitt, J.I. and Hocking, A.D. (1997) Fungi and Food Spoilage. 2nd Edition, Blacki Academic Press, London. http://dx.doi.org/10.1007/978-1-4615-6391-4
[29]	Lehmann, P.F., Lin, D. and Lasker, B.A. (1992) Genotypic Identification and Characterization of Species and Strains within the Genus Candida by Using Random Amplified Polymorphic DNA. Journal of Clinical Microbiology, 30, 3249-3254.
[30]	Anonymous (1999) Molecular Analyst Software, Fingerprinting Plus and Fingerprinting DST Software, Version 1.6, Instruction Manual. Bio-Rad Laboratories, Life Science Group, Hercules, Calif.
[31]	Askar, M.M.S., Youssri, M., Ahmed, F. and Ramadan, M. (2009) Chemical Characteristics and Antioxidant Activity of Exopolysaccharide Fractions from Microbacterium terregens. Carbohydrate Polymers, 77, 563-567. http://dx.doi.org/10.1016/j.carbpol.2009.01.037
[32]	Helal, A.G. (2006) Bioconversion of Straw into Improved Fodder: Preliminary Treatment of Rice Straw Using Me- chanical, Chemical and/or Gamma Irradiation. Mycobiology, 34, 14-21. http://dx.doi.org/10.4489/MYCO.2006.34.1.014
[33]	Irfan, M., Gulsher, M., Abbas, S., Syed, Q., Nadeem, M. and Baig, S. (2011) Effect of Various Pretreatment Condi- tions on Enzymatic Saccharification. Songklanakarin Journal of Science & Technology, 33, 397-404.
[34]	Abo-State, M.A.M., Hammad, A.I., Swelim, M. and Gannam, R.B. (2010) Some Critical Factors Affecting Cellulase(s) Production by Aspergillus terreus MAM-F23 and Aspergillus flavus MAM-F35 under Solid-State Fermentation of Wheat Straw. World Applied Sciences Journal, 9, 1171-1179.
[35]	Srilekha Yadav, K., Naseeruddin, S., Prashanthi, G., Sateesh, L. and Venkateswar, R. (2011) Bioethanol Fermentation of Concentrated Rice Straw Hydrolysate Using Co-Culture of Saccharomyces cerevisiae and Pichia stipitis. Biore- source Technology, 102, 6473-6478. http://dx.doi.org/10.1016/j.biortech.2011.03.019
[36]	Kumar, S.A. and Pushpa, A. (2012) Saccharification by Fungi and Ethanol Production by Bacteria Using Lignocellulosic Materials. International Research Journal of Pharmacy, 3, 411-414.
[37]	Patel, S.J., Onkarappa, R. and Gurumurthy, S.B. (2012) Ethanol Production from Lignocelluloses Hydrolyzates by Different Yeasts. Asian Journal of Experimental Biological Sciences, 3, 350-354.
[38]	Zayed, G. and Meyer, O. (1996) The Single-Batch Bioconversion of Wheat Straw to Ethanol Employing the Fungus Trichoderma viride and the Yeast Pachysolen tannophylu. Applied Microbiology and Biotechnology, 45, 551-555.
[39]	Alfenore, C., Molina, C., Guillouet, S.E., Uribelarrea, J.L., Goma, G. and Benbadis, L. (2002) Improving Ethanol Pro- duction and Viability of Saccharomyces cerevisiae by a Vitamin Feeding Strategy during Fed Batch Process. Applied Microbiology and Biotechnology, 60, 67-72. http://dx.doi.org/10.1007/s00253-002-1092-7
[40]	Mutalik, S., Vinodkumar, C.S., Swamy, S. and Manjappa, S. (2011) Depolymerization of Bagasse by Ruminococcus albus in the Production of Eco-Friendly Fuel. Research in Biotechnology, 2, 1-6.
[41]	Cheng, K.K., Cai, Y.B., Zhang, A.J., Ling, Z.H., Zhou, J.Y., Ge, P.J. and Xu, M.J. (2008) Sugarcane Bagasse Hemi- cellulose Hydrolysate for Ethanol Production by Acid Recovery Process. Biochemical Engineering Journal, 38, 105- 109. http://dx.doi.org/10.1016/j.bej.2007.07.012
[42]	Nigam, N.J. (2001) Ethanol Production from Wheat Straw Hemicelluloses Hydrolysate by Pichia stipitis. Journal of Biotechnology, 87, 17-27. http://dx.doi.org/10.1016/S0168-1656(00)00385-0
[43]	Jeffries, T.W. (1981) Conversion of Xylose to Ethanol under Aerobic Conditions by Candida tropicalis. Biotechnology Letters, 3, 213-218. http://dx.doi.org/10.1007/BF00154647
[44]	Girio, F.M., Fonseca, C., Carvalheiro, F., Duarte, L.C., Marques, S. and Bogel-Lukasik, R. (2010) Hemicelluloses for Fuel Ethanol: A Review. Bioresource Technology, 101, 4775-4800. http://dx.doi.org/10.1016/j.biortech.2010.01.088
[45]	Salvachua, D., Prieto, A., Lopez-Abelairas, M., Lu-Chau, T., Martinaz, A.T. and Martinez, M.J. (2011) Fungal Pre- treatment: An Alternative in Second-Generation Ethanol from Wheat Straw. Bioresource Technology, 102, 7500-7506. http://dx.doi.org/10.1016/j.biortech.2011.05.027
[46]	Senthilguru, K., George, T., Vasanthi, N. and Kannan, K. (2011) Ethanol Production from Lignocellulosic Waste. World Journal of Science and Technology, 1, 12-16.