Forward-Light-Scattering Characterization of Pre-Crystalline Aggregates in Crystallizing Lysozyme Solutions

DOI: 10.4236/ajac.2014.59065   PDF   HTML   XML   4,297 Downloads   5,113 Views   Citations


We present a method to characterize lysozyme pre-crystalline aggregates using a forward-light-scattering technique, which is highly sensitive to protein aggregates. The static light scattering properties at small angles of crystallizing lysozyme aggregates are discussed, and related to the crystallization conditions based on the concentration of added precipitant NaCl. Lysozyme solutions that started to crystallize because of precipitant exhibited profiles of forward light scattering that could be fitted by non-integer power laws, which indicated fractal aggregations of lysozyme had formed. Pre-crystalline solutions, in which lysozyme crystals later grew, had dense structural fractal clusters with fractal dimensions of D > 1.5. In contrast, solutions with aggregates in which crystals did not grow, had forward-light-scattering profiles that deviated from a power law or had lower power values.

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Wakamatsu, T. (2014) Forward-Light-Scattering Characterization of Pre-Crystalline Aggregates in Crystallizing Lysozyme Solutions. American Journal of Analytical Chemistry, 5, 581-588. doi: 10.4236/ajac.2014.59065.

Conflicts of Interest

The authors declare no conflicts of interest.


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